Artículo
Velasquez, S.M.; Ricardi, M.M.; Dorosz, J.G.; Fernandez, P.V.; Nadra, A.D.; Pol-Fachin, L.; Egelund, J.; Gille, S.; Harholt, J.; Ciancia, M.; Verli, H.; Pauly, M.; Bacic, A.; Olsen, C.E.; Ulvskov, P.; Petersen, B.L.; Somerville, C.; Iusem, N.D.; Estevez, J.M. "O-glycosylated cell wall proteins are essential in root hair growth" (2011) Science. 332(6036):1401-1403
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Abstract:
Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.
Registro:
| Documento: | Artículo |
| Título: | O-glycosylated cell wall proteins are essential in root hair growth |
| Autor: | Velasquez, S.M.; Ricardi, M.M.; Dorosz, J.G.; Fernandez, P.V.; Nadra, A.D.; Pol-Fachin, L.; Egelund, J.; Gille, S.; Harholt, J.; Ciancia, M.; Verli, H.; Pauly, M.; Bacic, A.; Olsen, C.E.; Ulvskov, P.; Petersen, B.L.; Somerville, C.; Iusem, N.D.; Estevez, J.M. |
| Filiación: | Inst. de Fisiol., Biologia Molec. Y Neurociencias-Consejo Nac. de Invest. Cientificas Y Tec., Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina Depto. de Biologia Aplicada Y Alimentos (Centro de Investigaciones en Hidratos de Carbono-CONICET), Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires CD1417DSE, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina Programa de Pos-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, 91500-979, United Kingdom Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Brazil Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Copenhagen 1871, Denmark Energy Biosciences Institute, University of California, Berkeley, Berkeley, CA 94720, United States Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Melbourne, VIC 3010, Australia Department of Natural Sciences, Faculty of Life Sciences, University of Copenhagen, 1871, Denmark Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina |
| Palabras clave: | arabinogalactan; arabinosyltransferase; cell membrane protein; extensin; glycan; glycoprotein; green fluorescent protein; hydroxyproline rich glycoprotein; procollagen proline 2 oxoglutarate 4 dioxygenase; proline rich protein; transferase; unclassified drug; adsorption; dicotyledon; enzyme activity; hair; inhibition; polysaccharide; protein; Arabidopsis; arabinosylation; article; cell growth; cell wall; chemical analysis; chemical reaction; glycosylation; hydroxylation; nonhuman; priority journal; protein expression; protein function; root hair; Arabidopsis; Arabidopsis Proteins; Arabinose; Carbohydrate Conformation; Cell Wall; Gene Expression Regulation, Plant; Genes, Plant; Glycoproteins; Glycosylation; Glycosyltransferases; Hydroxylation; Hydroxyproline; Models, Biological; Mutation; Pentosyltransferases; Phenotype; Plant Proteins; Plant Roots; Polysaccharides; Procollagen-Proline Dioxygenase; Proline; Protein Conformation; Protein Processing, Post-Translational; Protein Structure, Secondary; Arabidopsis thaliana |
| Año: | 2011 |
| Volumen: | 332 |
| Número: | 6036 |
| Página de inicio: | 1401 |
| Página de fin: | 1403 |
| DOI: | http://dx.doi.org/10.1126/science.1206657 |
| Título revista: | Science |
| Título revista abreviado: | Science |
| ISSN: | 00368075 |
| CODEN: | SCIEA |
| CAS: | arabinogalactan, 9036-66-2; procollagen proline 2 oxoglutarate 4 dioxygenase, 9028-06-2; transferase, 9047-61-4; Arabidopsis Proteins; Arabinose, 147-81-9; Glycoproteins; Glycosyltransferases, 2.4.-; Hydroxyproline, 51-35-4; Pentosyltransferases, 2.4.2.-; Plant Proteins; Polysaccharides; Procollagen-Proline Dioxygenase, 1.14.11.2; Proline, 147-85-3; arabinosyltransferase, 2.4.2.-; extensin protein, plant |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00368075_v332_n6036_p1401_Velasquez |
Referencias:
- Cosgrove, D.J., (2005) Nat. Cell Biol. Rev, 6, p. 850
- Somerville, C., (2004) Science, 306, p. 2206
- Xu, J.F., Tan, L., Lamport, D.T., Showalter, A.M., Kieliszewski, M.J., (2008) Phytochemistry, 69, p. 1631
- Kieliszewski, M.J., Lamport, D.T.A., (1994) Plant J., 5, p. 157
- Showalter, A.M., Keppler, B., Lichtenberg, J., Gu, D., Welch, L.R., (2010) Plant Physiol., 153, p. 485
- Tiainen, P., Myllyharju, J., Koivunen, P., (2005) J. Biol. Chem., 280, p. 1142
- Yuasa, K., Toyooka, K., Fukuda, H., Matsuoka, K., (2005) Plant J., 41, p. 81
- Petersen, B.L., (2011) Annu. Plant. Rev, 41, p. 305
- Egelund, J., (2007) Plant Mol. Biol., 64, p. 439
- Gille, S., Hänsel, U., Ziemann, M., Pauly, M., (2009) Proc. Natl. Acad. Sci. U.S.A., 106, p. 14699
- Shpak, E., Leykam, J.F., Kieliszewski, M.J., (1999) Proc. Natl. Acad. Sci. U.S.A., 96, p. 14736
- Cannon, M.C., (2008) Proc. Natl. Acad. Sci. U.S.A., 105, p. 2226
- Held, M.A., (2004) J. Biol. Chem., 279, p. 55474
- Estévez, J.M., Kieliszewski, M.J., Khitrov, N., Somerville, C., (2006) Plant Physiol., 142, p. 458
- Majamaa, K., Günzler, V., Hanauske-Abel, H.M., Myllylä, R., Kivirikko, K.I., (1986) J. Biol. Chem., 261, p. 7819
- Barnett, N., (1970) Plant Physiol., 45, p. 188
- Science Online, , Materials and methods are available as supporting material on
- Birnbaum, K.D., (2003) Science, 302, p. 1956
- Brady, S.M., (2007) Science, 318, p. 801
- Kieliszewski, M.J., (2001) Phytochemistry, 57, p. 319
- Ritzenthaler, C., (2002) Plant Cell, 14, p. 237
- Stafstrom, J.P., Staehelin, L.A., (1986) Plant Physiol., 81, p. 242
- Owens, N.W., Stetefeld, J., Lattová, E., Schweizer, F., (2010) J. Am. Chem. Soc., 132, p. 5036
- Price, N.J., (2003) J. Biol. Chem., 278, p. 41389
- Baumberger, N., (2003) Plant Physiol., 131, p. 1313
- Baumberger, N., Steiner, M., Ryser, U., Keller, B., Ringli, C., (2003) Plant J., 35, p. 71
- Won, S.-K., (2009) Plant Physiol., 150, p. 1459
- Lamport, D.T.A., Kieliszewski, M.J., Chen, Y., Cannon, M.C., (2011) Plant Physiol., 156, p. 11
- Ringli, C., (2010) Plant J., 63, p. 662
- Yi, K., Menand, B., Bell, E., Dolan, L., (2010) Nat. Genet., 42, p. 264
- Immerzeel, P., Eppink, M.M., De Vries, S.C., Schols, H.A., Voragen, A.G.J., (2006) Physiol. Plant., 128, p. 18
- Valentin, R., (2010) Langmuir, 26, p. 9891
Citas:
---------- APA ----------
Velasquez, S.M., Ricardi, M.M., Dorosz, J.G., Fernandez, P.V., Nadra, A.D., Pol-Fachin, L., Egelund, J.,..., Estevez, J.M. (2011) . O-glycosylated cell wall proteins are essential in root hair growth. Science, 332(6036), 1401-1403.http://dx.doi.org/10.1126/science.1206657
---------- CHICAGO ----------
Velasquez, S.M., Ricardi, M.M., Dorosz, J.G., Fernandez, P.V., Nadra, A.D., Pol-Fachin, L., et al. "O-glycosylated cell wall proteins are essential in root hair growth" . Science 332, no. 6036 (2011) : 1401-1403.http://dx.doi.org/10.1126/science.1206657
---------- MLA ----------
Velasquez, S.M., Ricardi, M.M., Dorosz, J.G., Fernandez, P.V., Nadra, A.D., Pol-Fachin, L., et al. "O-glycosylated cell wall proteins are essential in root hair growth" . Science, vol. 332, no. 6036, 2011, pp. 1401-1403.http://dx.doi.org/10.1126/science.1206657
---------- VANCOUVER ----------
Velasquez, S.M., Ricardi, M.M., Dorosz, J.G., Fernandez, P.V., Nadra, A.D., Pol-Fachin, L., et al. O-glycosylated cell wall proteins are essential in root hair growth. Science. 2011;332(6036):1401-1403.http://dx.doi.org/10.1126/science.1206657
