Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.
Documento: | Artículo |
Título: | The genome sequence of Trypanosoma cruzi, etiologic agent of chagas disease |
Autor: | El-Sayed, N.M.; Myler, P.J.; Bartholomeu, D.C.; Nilsson, D.; Aggarwal, G.; Tran, A.-N.; Ghedin, E.; Worthey, E.A.; Delcher, A.L.; Blandin, G.; Westenberger, S.J.; Caler, E.; Cerqueira, G.C.; Branche, C.; Haas, B.; Anupama, A.; Arner, E.; Åslund, L.; Attipoe, P.; Bontempi, E.; Bringaud, F.; Burton, P.; Cadag, E.; Campbell, D.A.; Carrington, M.; Crabtree, J.; Darban, H.; Da Silveira, J.F.; De Jong, P.; Edwards, K.; Englund, P.T.; Fazelina, G.; Feldblyum, T.; Ferella, M.; Frasch, A.C.; Gull, K.; Horn, D.; Hou, L.; Huang, Y.; Kindlund, E.; Klingbeil, M.; Kluge, S.; Koo, H.; Lacerda, D.; Levin, M.J.; Lorenzi, H.; Louie, T.; Machado, C.R.; McCulloch, R.; McKenna, A.; Mizuno, Y.; Mottram, J.C.; Nelson, S.; Ochaya, S.; Osoegawa, K.; Pai, G.; Parsons, M.; Pentony, M.; Pettersson, U.; Pop, M.; Ramirez, J.L.; Rinta, J.; Robertson, L.; Salzberg, S.L.; Sanchez, D.O.; Seyler, A.; Sharma, R.; Shetty, J.; Simpson, A.J.; Sisk, E.; Tammi, M.T.; Tarleton, R.; Teixeira, S.; Van Aken, S.; Vogt, C.; Ward, P.N.; Wickstead, B.; Wortman, J.; White, O.; Fraser, C.M.; Stuart, K.D.; Andersson, B. |
Filiación: | Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, United States Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20052, United States Seattle Biomedical Research Institute, Seattle, WA 98109, United States Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, United States Department of Medical Education and Biomedical Informatics, University of Washington, Seattle, WA 98195, United States Center for Genomics and Bioinformatics, Karolinska Institutet, Berzelius väg 35, S-171 77 Stockholm, Sweden Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, United States Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden Instítuto Nacional de Parasitología Dr. M. Fatala Chabén, Administración Nacional de Laboratories e Insitutos de Salud (ANLIS), 1063, Buenos Aires, Argentina Laboratoire de Génomique Fonctionnelle des Trypanosomatides, UMR-CNRS 5162, Université Victor Segalen Bordeaux II, 33076 Bordeaux Cedex, France Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow G11 6NU, United Kingdom Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, CEP 04023-062, São Paulo, SP, Brazil BACPAC Resources, Children's Hospital Oakland Research Institute, Oakland, CA 94609, United States Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico de Chascomus, National University of San Martin, National Research Council, 1650 Buenos Aires, Argentina Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, United Kingdom Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States René Rachou Research Center, CPqRR, Oswaldo Cruz Foundation, Belo Horizonte, MG, Brazil Laboratory of Molecular Biology of Chagas Disease, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Centro de Genomica Aplicada-CeGA-University of Buenos Aires, 1428 Buenos Aires, Argentina Instituto de Biología Experimental, Universidad Central de Venezuela and ADEA-MCT, 1041-A Caracas, Venezuela Department of Biological Sciences, National University of Singapore, Singapore, Singapore Center for Tropical and Emerging Global Diseases, Department of Cellular Biology, University of Georgia, Athens, GA 30602, United States |
Palabras clave: | Diseases; DNA; Genes; Proteins; Chagas diseases; Surface molecules; Trypanosoma cruzi; Protozoa; gene product; membrane protein; mitochondrial DNA; mucin; phosphatase; protein kinase; protozoal protein; sialidase; genome; allele; article; Chagas disease; diploidy; DNA repair; DNA replication; eukaryote; gene sequence; genome analysis; Leishmania major; molecular size; multigene family; nonhuman; priority journal; retroposon; signal transduction; Trypanosoma brucei; Trypanosoma cruzi; Animals; Chagas Disease; DNA Repair; DNA Replication; DNA, Mitochondrial; DNA, Protozoan; Genes, Protozoan; Genome, Protozoan; Humans; Meiosis; Membrane Proteins; Multigene Family; Protozoan Proteins; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Retroelements; Sequence Analysis, DNA; Signal Transduction; Telomere; Trypanocidal Agents; Trypanosoma cruzi; Eukaryota; Leishmania major; Protozoa; Trypanosoma cruzi |
Año: | 2005 |
Volumen: | 309 |
Número: | 5733 |
Página de inicio: | 409 |
Página de fin: | 415+435 |
DOI: | http://dx.doi.org/10.1126/science.1112631 |
Título revista: | Science |
Título revista abreviado: | Science |
ISSN: | 00368075 |
CODEN: | SCIEA |
CAS: | phosphatase, 9013-05-2; protein kinase, 9026-43-1; sialidase, 9001-67-6; DNA, Mitochondrial; DNA, Protozoan; Membrane Proteins; Protozoan Proteins; Retroelements; Trypanocidal Agents |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00368075_v309_n5733_p409_ElSayed |