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Abstract:

Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase β-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. © 2011 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
Autor:Pavan, M.E.; Robles, C.; Cairó, F.M.; Marcellino, R.; Pettinari, M.J.
Filiación:Biochemiq S.A., Laboratorio de Biología Molecular, Buenos Aires, Argentina
National Institute for Agricultural Technology (INTA), Animal Health Group, Bariloche, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Caseous lymphadenitis; Corynebacterium pseudotuberculosis; RpoB PCR-RFLP; endonuclease; protein MseI; protein StuI; unclassified drug; analytic method; Arcanobacterium pyogenes; article; bacterial gene; bacterium identification; bacterium isolation; computer analysis; controlled study; Corynebacterium pseudotuberculosis; Corynebacterium ulcerans; molecular typing; nonhuman; nucleotide sequence; polymerase chain reaction; reliability; restriction fragment length polymorphism; RNA polymerase beta subunit gene; rpoB gene; sequence analysis; sheep; species differentiation; unindexed sequence; wild type; Animals; Corynebacterium Infections; Corynebacterium pseudotuberculosis; DNA-Directed RNA Polymerases; Polymorphism, Restriction Fragment Length; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis; Sheep; Sheep Diseases; Animalia; Arcanobacterium pyogenes; Capra; Corynebacterium; Corynebacterium pseudotuberculosis; Ovis; Ovis aries
Año:2012
Volumen:92
Número:2
Página de inicio:202
Página de fin:206
DOI: http://dx.doi.org/10.1016/j.rvsc.2011.02.007
Título revista:Research in Veterinary Science
Título revista abreviado:Res. Vet. Sci.
ISSN:00345288
CODEN:RVTSA
CAS:endonuclease, 9055-11-2; DNA-Directed RNA Polymerases, 2.7.7.6; RNA polymerase beta subunit, 2.7.7.6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v92_n2_p202_Pavan

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Citas:

---------- APA ----------
Pavan, M.E., Robles, C., Cairó, F.M., Marcellino, R. & Pettinari, M.J. (2012) . Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB). Research in Veterinary Science, 92(2), 202-206.
http://dx.doi.org/10.1016/j.rvsc.2011.02.007
---------- CHICAGO ----------
Pavan, M.E., Robles, C., Cairó, F.M., Marcellino, R., Pettinari, M.J. "Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)" . Research in Veterinary Science 92, no. 2 (2012) : 202-206.
http://dx.doi.org/10.1016/j.rvsc.2011.02.007
---------- MLA ----------
Pavan, M.E., Robles, C., Cairó, F.M., Marcellino, R., Pettinari, M.J. "Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)" . Research in Veterinary Science, vol. 92, no. 2, 2012, pp. 202-206.
http://dx.doi.org/10.1016/j.rvsc.2011.02.007
---------- VANCOUVER ----------
Pavan, M.E., Robles, C., Cairó, F.M., Marcellino, R., Pettinari, M.J. Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB). Res. Vet. Sci. 2012;92(2):202-206.
http://dx.doi.org/10.1016/j.rvsc.2011.02.007