Artículo
Vezzani, D.; Fontanarrosa, M.F.; Eiras, D.F. "Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results" (2008) Research in Veterinary Science. 85(1):113-115
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Abstract:
The aim of this study was to evaluate the performance of commercial heartworm antigen tests in dogs harbouring Dirofilaria immitis microfilariae near its distribution limit in South America. A total of 4934 blood samples of adult dogs from Southern Greater Buenos Aires were examined to detect circulating microfilariae in the buffy coat interface between December 2005 and April 2006. Microfilariae were detected in 88 (1.8%) blood samples and all the microfilariae observed were identified as D. immitis by acid phosphatase stain technique. In a first trial, 69 (78.4%) out of the 88 serum were positive by Speed® Diro. Then, a new test was performed over 25 microfilariae-positive serum samples randomly selected among the 88 previously tested samples and using simultaneously Speed® Diro, Witness® Dirofilaria and Snap® 3dx. This second trial showed identical results for the three different tests, in which 19 (76%) samples were positive. Therefore, more than 20% of microfilaremic dogs were antigen negative. The main hypothesis that could explain our finding is a low worm burden in the study area. According to our preliminary results, it is highly recommendable the complementary use of antigen tests and other procedures to obtain an accurate diagnostic near the distribution limit of the parasite. © 2007 Elsevier Ltd. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results |
| Autor: | Vezzani, D.; Fontanarrosa, M.F.; Eiras, D.F. |
| Filiación: | Ecología de Reservorios y Vectores de Parásitos, Facultad de Cs. Exactas y Naturales, Universidad de Buenos Aires, Argentina Laboratorio DIAP (Diagnóstico en Animales Pequeños), Inca 109, (B1836BBC), Llavallol, Buenos Aires, Argentina Cátedra de Parasitología y Enfermedades Parasitarias, Departamento de Microbiología, Facultad de Ciencias Veterinarias, CC 296, B1900AVW La Plata, Argentina |
| Palabras clave: | Argentina; Commercial heartworm antigen test kits; Dirofilaria immitis; Efficiency; acid phosphatase; parasite antigen; animal parasitosis; antigen detection; Argentina; article; controlled study; diagnostic accuracy; diagnostic value; Dirofilaria immitis; dog; hypothesis; immunoassay; intermethod comparison; microfilariasis; nonhuman; parasite identification; parasite prevalence; Animals; Dirofilariasis; Dog Diseases; Dogs; Predictive Value of Tests; Reagent Kits, Diagnostic; South America; Canis familiaris; Dirofilaria; Dirofilaria immitis |
| Año: | 2008 |
| Volumen: | 85 |
| Número: | 1 |
| Página de inicio: | 113 |
| Página de fin: | 115 |
| DOI: | http://dx.doi.org/10.1016/j.rvsc.2007.08.001 |
| Título revista: | Research in Veterinary Science |
| Título revista abreviado: | Res. Vet. Sci. |
| ISSN: | 00345288 |
| CODEN: | RVTSA |
| CAS: | acid phosphatase, 9001-77-8, 9025-88-1; Reagent Kits, Diagnostic |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v85_n1_p113_Vezzani |
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Citas:
---------- APA ----------
Vezzani, D., Fontanarrosa, M.F. & Eiras, D.F. (2008) . Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results. Research in Veterinary Science, 85(1), 113-115.http://dx.doi.org/10.1016/j.rvsc.2007.08.001
---------- CHICAGO ----------
Vezzani, D., Fontanarrosa, M.F., Eiras, D.F. "Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results" . Research in Veterinary Science 85, no. 1 (2008) : 113-115.http://dx.doi.org/10.1016/j.rvsc.2007.08.001
---------- MLA ----------
Vezzani, D., Fontanarrosa, M.F., Eiras, D.F. "Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results" . Research in Veterinary Science, vol. 85, no. 1, 2008, pp. 113-115.http://dx.doi.org/10.1016/j.rvsc.2007.08.001
---------- VANCOUVER ----------
Vezzani, D., Fontanarrosa, M.F., Eiras, D.F. Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results. Res. Vet. Sci. 2008;85(1):113-115.http://dx.doi.org/10.1016/j.rvsc.2007.08.001
