Abstract:
This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ERα in corticotrophs. Females had a greater proportion of corticotrophs per field (p < 0.01), with a greater cellular area and optical density (p < 0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p < 0.001). In zona fasciculata, ERα and MC2R expression was greater in females (p < 0.001) and was greater in winter (p < 0.001). ERα was identified in corticotrophs. This study is the first to demonstrate ERα expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations. © 2015 Elsevier Ltd.
Registro:
Documento: |
Artículo
|
Título: | Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
Autor: | Gallelli, M.F.; Lombardo, D.; Vissio, P.; Quiroga, A.; Caggiano, N.; Soler, E.; Meikle, A.; Castillo, V.A. |
Filiación: | UBACyT scholarship holder, Argentina Hospital Escuela-Unidad de Endocrinología, A. Clínica Médica de Pequeños Animales, Fac. de Ciencias Veterinarias-UBA, Av. Chorroarin 280, Buenos Aires, CP 1427, Argentina Cátedra de Histología, Fac. de Ciencias Veterinarias-UBA, Av. Chorroarin 280, Buenos Aires CP, 1427, Argentina Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA/IBBEA-CONICET-UBA, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina Área de Patología Especial, Fac. De Ciencias Veterinarias, UNLP, Calle 60 y 118, CP Buenos Aires, 1900, Argentina Laboratorio de Técnicas Nucleares, Fac. De Ciencias Veterinarias, UDELAR, Lasplacesr 1550-1620, CP Montevideo, 11600, Uruguay
|
Palabras clave: | Adrenal cortex; Corticotroph; Melanocortin 2 receptor (MC2R); Oestrogen receptor; Sexual dimorphism; corticotropin; estrogen receptor alpha; melanocortin 2 receptor; estrogen receptor alpha; ACTH secreting cell; animal tissue; Article; controlled study; dog; female; immunohistochemistry; male; nonhuman; optical density; protein analysis; protein expression; seasonal variation; sex difference; summer; winter; zona fasciculata; anatomy and histology; animal; chemistry; genetics; hypophysis adrenal system; hypothalamus hypophysis system; metabolism; physiology; season; sexual development; veterinary; Animals; Dogs; Estrogen Receptor alpha; Female; Hypothalamo-Hypophyseal System; Immunohistochemistry; Male; Pituitary-Adrenal System; Seasons; Sex Characteristics |
Año: | 2016
|
Volumen: | 104
|
Página de inicio: | 10
|
Página de fin: | 16
|
DOI: |
http://dx.doi.org/10.1016/j.rvsc.2015.11.003 |
Título revista: | Research in Veterinary Science
|
Título revista abreviado: | Res. Vet. Sci.
|
ISSN: | 00345288
|
CODEN: | RVTSA
|
CAS: | corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; Estrogen Receptor alpha
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v104_n_p10_Gallelli |
Referencias:
- Amirat, Z., Brudieux, R., Seasonal changes in in vivo cortisol response to ACTH and in plasma and pituitary concentrations of ACTH in a desert rodent, the sand rat (Psammomys obesus) (1993) Comp. Biochem. Physiol., 104, pp. 29-34
- Burgess, L.H., Handa, R.J., Estrogen-induced alterations in the regulation of mineralocorticoid and glucocorticoid receptor messenger RNA expression in the female rat anterior pituitary gland and brain (1993) Mol. Cell. Neurosci., 4, pp. 191-198
- Cordero, M., Brorsen, B.W., McFarlane, D., Circadian and circannual rhythms of cortisol, ACTH, and α-melanocyte-stimulating hormone in healthy horses (2012) Domest. Anim. Endocrinol., 43, pp. 317-324
- Cutler, G.B., Barnes, K.M., Sauer, M.A., Loriaux, D.L., Estrogen receptor in rat adrenal gland (1978) Endocrinology, 102, pp. 252-257
- Denef, C., Paracrinicity: the story of 30 years of cellular pituitary crosstalk (2008) J. Neuroendocrinol., 20, pp. 1-70
- Donaldson, M.T., McDonnell, S.M., Schanbacher, B.J., Lamb, S.V., McFarlane, D., Beech, J., Variation in plasma adrenocorticotropic hormone concentration and dexamethasone suppression test results with season, age, and sex in healthy ponies and horses (2005) J. Vet. Intern. Med., 19, pp. 217-222
- Figueiredo, H.F., Ulrich-Lai, Y.M., Choi, D.C., Herman, J.P., Estrogen potentiates adrenocortical responses to stress in female rats (2007) Am. J. Physiol. Endocrinol. Metab., 292, pp. 1173-1182
- Filippa, V., Mohamed, F., ACTH cells of pituitary pars distalis of viscacha (Lagostomus maximus maximus): immunohistochemical study in relation to season, sex, and growth (2006) Gen. Comp. Endocrinol., 146, pp. 217-225
- Gallelli, M.F., Monachesi, N., Miceli, D., Cabrera Blatter, M.F., Gómez, N., Meikle, A., Castillo, V., Plasma ACTH, α-MSH and cortisol variations in the dog during the oestrous cycle in different photoperiods (2015) Vet. Med., 60. , (article in press)
- Goel, N., Workman, J.L., Lee, T.T., Innala, L., Viau, V., Sex differences in the HPA axis (2014) Commun. Phys., 4, pp. 1121-1155
- Handa, L.J., Burgess, L.H., Kerr, J.E., O'Keefe, J.A., Gonadal steroid hormone receptors and sex differences in the hypothalamo-pituitary-adrenal axis (1994) Horm. Behav., 28, pp. 464-476
- Handa, R.J., Weiser, M.J., Gonadal steroid hormones and the hypothalamo-pituitary-adrenal axis (2014) Front. Neuroendocrinol., 35, pp. 97-220
- Hira, Y., Sakai, Y., Matsushima, S., Effects of photoperiod and melatonin on the development of growth hormone cells and the pituitary-adrenal axis in the Djungarian hamster, Phodopus sungorus (2001) Arch. Histol. Cytol., 64, pp. 211-222
- Ingram, J.R., Crockford, J.N., Matthews, L.R., Ultradian, circadian and seasonal rhythms in cortisol secretion and adrenal responsiveness to ACTH and yarding in unrestrained red deer (Cervus elaphus) stags (1999) J. Endocrinol., 162, pp. 289-300
- Ithurralde, J., Costas, A.L., Pessina, P., Cueto, E., Fila, D., Meikle, A., Immunohistochemical determination of estrogen receptor-α in canine vaginal biopsies throughout proestrus, estrus, and early diestrus (2013) Theriogenology, 80, pp. 805-811
- Iwasaki-Sekino, A., Mano-Otagiri, A., Ohata, H., Yamauchi, N., Shibasaki, T., Gender differences in corticotropin and corticosterone secretion and corticotropin-releasing factor mRNA expression in the paraventricular nucleus of the hypothalamus and central nucleus of the amygdala in response to footshock stress or psychological stress in rats (2009) Psychoneuroendocrinology, 34, pp. 226-237
- Larkin, J.W., Binks, S.L., Li, Y., Selvage, D., The role of oestradiol in sexually dimorphic hypothalamic-pituitary-adrenal axis responses to intracerebroventricular ethanol administration in the rat (2010) J. Neuroendocrinol., 22, pp. 24-32
- Levin, E.R., Integration of the extranuclear and nuclear actions of estrogen (2005) Mol. Endocrinol., 19, pp. 1951-1959
- Lo, M.J., Chang, L.L., Wang, P.S., Effects of estradiol on corticosterone secretion in ovariectomized rats (2000) J. Cell. Biochem., 77, pp. 560-568
- Mc Cormick, C.M., Furey, B.F., Child, M., Sawyer, M.J., Donohue, S.M., Neonatal sex hormones have "organizational" effects on the hypothalamic-pituitary-adrenal axis of male rats (1998) Dev. Brain Res., 105, pp. 295-307
- Mitchner, N.A., Garlick, C., Ben-Jonathan, N., Cellular distribution and gene regulation of estrogen receptors a and b in the rat pituitary gland (1998) Endocrinology, 139, pp. 3976-3983
- Mitsushima, D., Masuda, J., Kimura, F., Sex differences in the stress induced release of acetylcholine in the hippocampus and corticosterona from the adrenal cortex in rats (2003) Neuroendocrinology, 78, pp. 234-240
- Mol, J.A., Meij, B.P., Pituitary function (2008) Clinical Biochemistry of Domestic Animals, pp. 567-568. , Academic Press, USA, J.J. Kaneko, J.W. Harvey, M.L. Bruss (Eds.)
- Oberholzer, M., Ostreicher, M., Christen, H., Bruhlmann, M., Methods in quantitative image analysis (1996) Histochem. Cell Biol., 105, pp. 333-355
- (2015), http://rsb.info.nih.gov/ij/docs/examples/calibration/index.html, (February 16). Retrieved from []; Panagiotakopoulos, L., Neigh, G., Development of the HPA axis: where and when do sex differences manifest? (2014) Front. Neuroendocrinol., 35, pp. 285-302
- Pessina, P., Fernandez-Foren, A., Cueto, E., Delucchi, L., Castillo, V., Meikle, A., Cortisol secretion alter adrenocorticotrophin (ACTH) and dexamethasone tests in healthy female and male dogs (2009) Acta Vet. Scand., 51, pp. 33-38
- Pramoda, S., Saidapur, S.K., Seasonal changes in the cytomorphology of hypophyseal ACTH cells in relation to the reproduction cycle of the female of the frog Rana tigerina (Daud.) (1991) Funct. Dev. Morphol., 1, pp. 47-50
- Rhodes, M.E., Rubin, R.T., Functional sex differences ("sexual diergism") of central nervous system cholinergic systems, vasopressin, and hypothalamic-pituitary-adrenal axis activity in mammals: a selective review (1999) Brain Res. Rev., 30, pp. 135-152
- Ribes, A.C., Mohamed, F., Dominguez, S., Delgado, M., Scardapane, L., Guzman, J., Piezzi, R., Probable effect of photoperiod on seasonal variation in the nuclear volume of the adrenal cortex of viscacha (Lagostomus maximus maximus) (1999) Braz. J. Med. Biol. Res., 32, pp. 1115-1120
- Romero, L.M., Seasonal changes in plasma glucocorticoid concentrations in free-living vertebrates (2002) Gen. Comp. Endocrinol., 128, pp. 1-24
- Turner, B.B., Sex difference in glucocorticoid binding in rat pituitary is oestrogen dependent (1990) Life Sci., 46, pp. 1399-1406
- van Lier, E., Meikle, A., Bielli, A., Akerberg, S., Forsberg, M., Sahlin, L., Sex differences in oestrogen receptor levels in adrenal glands of sheep during the breeding season (2003) Domest. Anim. Endocrinol., 25, pp. 373-387
- van Lier, E., Carriquiry, M., Meikle, A., Sex steroid modulation of cortisol secretion in sheep (2014) Animal, 8, pp. 960-967
- Vidal, S., Roman, A., Moya, L., Inmunohistochemical identification and morphometric study of ACTH cells of mink (Mustela vison) during growth and different stages of sexual activity in the adult (1995) Gen. Comp. Endocrinol., 100, pp. 18-26
- Viau, V., Bingham, B., Davis, J., Lee, P., Wong, M., Gender and puberty interact on the stress-induced activation of parvocellular neurosecretory neurons and corticotropin-releasing hormone messenger ribonucleic acid expression in the rat (2005) Endocrinology, 146, pp. 137-146
- Weiser, M.J., Handa, R.J., Estrogen impairs glucocorticoid dependent negative feedback on the hypothalamic-pituitary-adrenal axis via oestrogen receptor alpha within the hypothalamus (2009) Neuroscience, 159, pp. 883-895
Citas:
---------- APA ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., Meikle, A.,..., Castillo, V.A.
(2016)
. Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation. Research in Veterinary Science, 104, 10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003---------- CHICAGO ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., et al.
"Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation"
. Research in Veterinary Science 104
(2016) : 10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003---------- MLA ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., et al.
"Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation"
. Research in Veterinary Science, vol. 104, 2016, pp. 10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003---------- VANCOUVER ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., et al. Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation. Res. Vet. Sci. 2016;104:10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003