Artículo

Gallelli, M.F.; Lombardo, D.; Vissio, P.; Quiroga, A.; Caggiano, N.; Soler, E.; Meikle, A.; Castillo, V.A. "Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation" (2016) Research in Veterinary Science. 104:10-16
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Abstract:

This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ERα in corticotrophs. Females had a greater proportion of corticotrophs per field (p < 0.01), with a greater cellular area and optical density (p < 0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p < 0.001). In zona fasciculata, ERα and MC2R expression was greater in females (p < 0.001) and was greater in winter (p < 0.001). ERα was identified in corticotrophs. This study is the first to demonstrate ERα expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations. © 2015 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation
Autor:Gallelli, M.F.; Lombardo, D.; Vissio, P.; Quiroga, A.; Caggiano, N.; Soler, E.; Meikle, A.; Castillo, V.A.
Filiación:UBACyT scholarship holder, Argentina
Hospital Escuela-Unidad de Endocrinología, A. Clínica Médica de Pequeños Animales, Fac. de Ciencias Veterinarias-UBA, Av. Chorroarin 280, Buenos Aires, CP 1427, Argentina
Cátedra de Histología, Fac. de Ciencias Veterinarias-UBA, Av. Chorroarin 280, Buenos Aires CP, 1427, Argentina
Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA/IBBEA-CONICET-UBA, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Área de Patología Especial, Fac. De Ciencias Veterinarias, UNLP, Calle 60 y 118, CP Buenos Aires, 1900, Argentina
Laboratorio de Técnicas Nucleares, Fac. De Ciencias Veterinarias, UDELAR, Lasplacesr 1550-1620, CP Montevideo, 11600, Uruguay
Palabras clave:Adrenal cortex; Corticotroph; Melanocortin 2 receptor (MC2R); Oestrogen receptor; Sexual dimorphism; corticotropin; estrogen receptor alpha; melanocortin 2 receptor; estrogen receptor alpha; ACTH secreting cell; animal tissue; Article; controlled study; dog; female; immunohistochemistry; male; nonhuman; optical density; protein analysis; protein expression; seasonal variation; sex difference; summer; winter; zona fasciculata; anatomy and histology; animal; chemistry; genetics; hypophysis adrenal system; hypothalamus hypophysis system; metabolism; physiology; season; sexual development; veterinary; Animals; Dogs; Estrogen Receptor alpha; Female; Hypothalamo-Hypophyseal System; Immunohistochemistry; Male; Pituitary-Adrenal System; Seasons; Sex Characteristics
Año:2016
Volumen:104
Página de inicio:10
Página de fin:16
DOI: http://dx.doi.org/10.1016/j.rvsc.2015.11.003
Título revista:Research in Veterinary Science
Título revista abreviado:Res. Vet. Sci.
ISSN:00345288
CODEN:RVTSA
CAS:corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; Estrogen Receptor alpha
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v104_n_p10_Gallelli

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Citas:

---------- APA ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., Meikle, A.,..., Castillo, V.A. (2016) . Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation. Research in Veterinary Science, 104, 10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003
---------- CHICAGO ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., et al. "Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation" . Research in Veterinary Science 104 (2016) : 10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003
---------- MLA ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., et al. "Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation" . Research in Veterinary Science, vol. 104, 2016, pp. 10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003
---------- VANCOUVER ----------
Gallelli, M.F., Lombardo, D., Vissio, P., Quiroga, A., Caggiano, N., Soler, E., et al. Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation. Res. Vet. Sci. 2016;104:10-16.
http://dx.doi.org/10.1016/j.rvsc.2015.11.003