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Steiner, N.; Farias-Soares, F.L.; Schmidt, É.C.; Pereira, M.L.T.; Scheid, B.; Rogge-Renner, G.D.; Bouzon, Z.L.; Schmidt, D.; Maldonado, S.; Guerra, M.P. "Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze" (2016) Protoplasma. 253(2):487-501
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Abstract:

Somatic embryogenesis is a morphogenetic route useful for the study of embryonic development, as well as the large-scale propagation of endangered species, such as the Brazilian pine (Araucaria angustifolia). In the present study, we investigated the morphological and ultrastructural organization of A. angustifolia somatic embryo development by means of optical and electron microscopy. The proembryogenic stage was characterized by the proliferation of proembryogenic masses (PEMs), which are cellular aggregates composed of embryogenic cells (ECs) attached to suspensor-like cells (SCs). PEMs proliferate through three developmental stages, PEM I, II, and III, by changes in the number of ECs and SCs. PEM III-to-early somatic embryo (SE) transition was characterized by compact clusters of ECs growing out of PEM III, albeit still connected to it by SCs. Early SEs showed a dense globular embryonic mass (EM) and suspensor region (SR) connected by embryonic tube cells (TCs). By comparison, early somatic and zygotic embryos showed similar morphology. ECs are round with a large nucleus, nucleoli, and many cytoplasmic organelles. In contrast, TCs and SCs are elongated and vacuolated with cellular dismantling which is associated with programmed cell death of SCs. Abundant starch grains were observed in the TCs and SCs, while proteins were more abundant in the ECs. Based on the results of this study, a fate map of SE development in A. angustifolia is, for the first time, proposed. Additionally, this study shows the cell biology of SE development of this primitive gymnosperm which may be useful in evolutionary studies in this area. © 2015, Springer-Verlag Wien.

Registro:

Documento: Artículo
Título:Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze
Autor:Steiner, N.; Farias-Soares, F.L.; Schmidt, É.C.; Pereira, M.L.T.; Scheid, B.; Rogge-Renner, G.D.; Bouzon, Z.L.; Schmidt, D.; Maldonado, S.; Guerra, M.P.
Filiación:Plant Physiology Laboratory, Department of Botany, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil
Graduate Program in Plant Genetic Resources, Department of Plant Science, Federal University of Santa Catarina, C.P. 476, Florianópolis, SC 88040-900, Brazil
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, C.P. 476, Florianópolis, SC 88049-900, Brazil
Laboratory of Plant Developmental Physiology and Genetics, Department of Plant Science, Federal University of Santa Catarina, C.P. 476, Florianópolis, SC 88040-900, Brazil
Central Laboratory of Electron Microscopy, Federal University of Santa Catarina, Florianópolis, SC, Brazil
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, Pab. 2, Ciudad Universitaria, Buenos Aires, C1428EGA, Argentina
Department of Biological Sciences, University of Joinville Region, Joinville, SC, Brazil
Palabras clave:Conifers; Histochemistry; Pluripotency; Somatic embryogenesis; Suspensor cell; cell culture technique; growth, development and aging; plant seed; tree; ultrastructure; Culture Techniques; Seeds; Trees
Año:2016
Volumen:253
Número:2
Página de inicio:487
Página de fin:501
DOI: http://dx.doi.org/10.1007/s00709-015-0827-0
Título revista:Protoplasma
Título revista abreviado:Protoplasma
ISSN:0033183X
CODEN:PROTA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v253_n2_p487_Steiner

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Citas:

---------- APA ----------
Steiner, N., Farias-Soares, F.L., Schmidt, É.C., Pereira, M.L.T., Scheid, B., Rogge-Renner, G.D., Bouzon, Z.L.,..., Guerra, M.P. (2016) . Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze. Protoplasma, 253(2), 487-501.
http://dx.doi.org/10.1007/s00709-015-0827-0
---------- CHICAGO ----------
Steiner, N., Farias-Soares, F.L., Schmidt, É.C., Pereira, M.L.T., Scheid, B., Rogge-Renner, G.D., et al. "Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze" . Protoplasma 253, no. 2 (2016) : 487-501.
http://dx.doi.org/10.1007/s00709-015-0827-0
---------- MLA ----------
Steiner, N., Farias-Soares, F.L., Schmidt, É.C., Pereira, M.L.T., Scheid, B., Rogge-Renner, G.D., et al. "Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze" . Protoplasma, vol. 253, no. 2, 2016, pp. 487-501.
http://dx.doi.org/10.1007/s00709-015-0827-0
---------- VANCOUVER ----------
Steiner, N., Farias-Soares, F.L., Schmidt, É.C., Pereira, M.L.T., Scheid, B., Rogge-Renner, G.D., et al. Toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of Araucaria angustifolia (Bert.) O. Kuntze. Protoplasma. 2016;253(2):487-501.
http://dx.doi.org/10.1007/s00709-015-0827-0