Artículo

Farias-Soares, F.L.; Burrieza, H.P.; Steiner, N.; Maldonado, S.; Guerra, M.P. "Immunoanalysis of dehydrins in Araucaria angustifolia embryos" (2013) Protoplasma. 250(4):911-918
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Abstract:

The aim of this study was to describe the dehydrin content of mature Araucaria angustifolia embryos, a species of endangered and economically important conifers, native to southern Brazil, northeastern Argentina, and eastern Paraguay. The A. angustifolia seeds have been categorized as recalcitrant. Dehydrins were studied by western blot analysis and in situ immunolocalization microscopy using antibodies raised against the K segment, a highly conserved lysine-rich 15-amino acid sequence extensively used to recognize proteins immunologically related to the dehydrin family. Western blot analysis of the heat-stable protein fraction, as estimated by 15 % SDS-PAGE, revealed three main bands of approximately 20-, 26-, and 29-kDa; when 17.5 % SDS-PAGE was used, each band resolved into two other bands. Two thermosensitive dehydrin bands of around 16 and 35 kDa were common to the axis and cotyledons, and another thermosensitive band, with molecular mass of approximately 10 kDa, was present in the cotyledons only. Following alkaline phosphatase (AP) treatment, a gel mobility shift was detected for each one of the four main bands that can be due to phosphorylation. Dehydrins were detected in all axis and cotyledon tissues using in situ immunolocalization microscopy. At the subcellular level, dehydrins were immunolocalized in the nuclei, protein bodies, and microbodies. In the nucleus, dehydrins were found to be associated with chromatin. We concluded that the gel mobility shift for the four main bands (probably due to phosphorylation), the presence of thermosensitive bands, and the specific localizations in nuclei and protein bodies provide key starting points to understand the function of dehydrins in the embryo cells of this species. © 2012 Springer-Verlag Wien.

Registro:

Documento: Artículo
Título:Immunoanalysis of dehydrins in Araucaria angustifolia embryos
Autor:Farias-Soares, F.L.; Burrieza, H.P.; Steiner, N.; Maldonado, S.; Guerra, M.P.
Filiación:Plant Developmental Physiology and Genetics Laboratory Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina, 88034-000, Florianópolis, SC, Brazil
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina
Curitibanos Campus, Federal University of Santa Catarina, 89550-000, PO Box 101, Curitibanos, SC, Brazil
Palabras clave:Araucaria angustifolia seed; Dehydrin in situ immunolocalization; Dehydrin subcellular localization; Dehydrin western blot analysis; Phosphorylated dehydrins; Thermosensitive dehydrins; Araucaria angustifolia; Coniferophyta; dehydrin proteins, plant; vegetable protein; article; chemistry; conifer; metabolism; plant seed; Western blotting; Blotting, Western; Coniferophyta; Plant Proteins; Seeds
Año:2013
Volumen:250
Número:4
Página de inicio:911
Página de fin:918
DOI: http://dx.doi.org/10.1007/s00709-012-0474-7
Título revista:Protoplasma
Título revista abreviado:Protoplasma
ISSN:0033183X
CODEN:PROTA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v250_n4_p911_FariasSoares

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Citas:

---------- APA ----------
Farias-Soares, F.L., Burrieza, H.P., Steiner, N., Maldonado, S. & Guerra, M.P. (2013) . Immunoanalysis of dehydrins in Araucaria angustifolia embryos. Protoplasma, 250(4), 911-918.
http://dx.doi.org/10.1007/s00709-012-0474-7
---------- CHICAGO ----------
Farias-Soares, F.L., Burrieza, H.P., Steiner, N., Maldonado, S., Guerra, M.P. "Immunoanalysis of dehydrins in Araucaria angustifolia embryos" . Protoplasma 250, no. 4 (2013) : 911-918.
http://dx.doi.org/10.1007/s00709-012-0474-7
---------- MLA ----------
Farias-Soares, F.L., Burrieza, H.P., Steiner, N., Maldonado, S., Guerra, M.P. "Immunoanalysis of dehydrins in Araucaria angustifolia embryos" . Protoplasma, vol. 250, no. 4, 2013, pp. 911-918.
http://dx.doi.org/10.1007/s00709-012-0474-7
---------- VANCOUVER ----------
Farias-Soares, F.L., Burrieza, H.P., Steiner, N., Maldonado, S., Guerra, M.P. Immunoanalysis of dehydrins in Araucaria angustifolia embryos. Protoplasma. 2013;250(4):911-918.
http://dx.doi.org/10.1007/s00709-012-0474-7