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A comparative analysis of the effect of two compounds, dibutyryl-cyclic-AMP (dbcAMP) and latrunculin B, on the morphology and ultrastructure of the dimorphic fungus Mucor rouxii under aerobic growth conditions is presented. dbcAMP acts through the sustained activation of protein kinase A, and latrunculin B through the disruption of the actin cytoskeleton. Upon addition of these compounds to the growth medium at any stage of the germination process, cells lost polarised growth and switched to isodiametric growth. The effect was reversible. The morphologies, visualised by light microscopy or scanning electron microscopy (SEM), were alike. A switch from a rough to a smooth surface was observed by SEM when cells were repolarised by removal of the added compound. Ultrastructural changes under both conditions, as observed by transmission electron microscopy, were similar, the main feature being the enlargement of the cell wall, with irregular depositions, and detachment from the cell membrane. dbcAMP-treated cells showed a decrease in the number of glycogen granules compared with control and latrunculin B-treated cells. F-actin staining with fluorescein isothiocyanate-phalloidin showed that both dbcAMP- and latrunculin B-treated cells displayed a much lower fluorescence than control cells, with only a few pale plaques. The results suggest that the sustained activation of protein kinase A, which impairs polarised growth, might exert its effect through a modification of actin cytoskeleton organisation, very probably also involving an integrinlike pathway, as judged by the cell wall detachment and loss of cell adhesiveness of the dbcAMP-treated isodiametric cells. © Springer-Verlag 2006.


Documento: Artículo
Título:Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton
Autor:Pereyra, E.; Ingerfeld, M.; Anderson, N.; Jackson, S.L.; Moreno, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand
Departamento de Química Biológica, Pabellón 2, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Cell wall; Cyclic-AMP signal transduction; Cytoskeleton; Latrunculin; Mucor rouxii; Scanning electron microscopy; Transmission electron microscopy; actin; bucladesine; cyclic AMP; cyclic AMP dependent protein kinase; drug derivative; fused heterocyclic rings; latrunculin B; thiazolidine derivative; article; cytoskeleton; drug effect; fluorescence microscopy; metabolism; methodology; Mucor; scanning electron microscopy; transmission electron microscopy; ultrastructure; Actins; Bicyclo Compounds, Heterocyclic; Bucladesine; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Cytoskeleton; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Mucor; Thiazolidines; Amylomyces rouxii; Fungi; Mucor
Página de inicio:189
Página de fin:199
Título revista:Protoplasma
Título revista abreviado:Protoplasma
CAS:bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; latrunculin B, 76343-94-7; Actins; Bicyclo Compounds, Heterocyclic; Bucladesine, 362-74-3; Cyclic AMP, 60-92-4; Cyclic AMP-Dependent Protein Kinases,,; Thiazolidines; latrunculin B, 76343-94-7


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---------- APA ----------
Pereyra, E., Ingerfeld, M., Anderson, N., Jackson, S.L. & Moreno, S. (2006) . Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton. Protoplasma, 228(4), 189-199.
---------- CHICAGO ----------
Pereyra, E., Ingerfeld, M., Anderson, N., Jackson, S.L., Moreno, S. "Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton" . Protoplasma 228, no. 4 (2006) : 189-199.
---------- MLA ----------
Pereyra, E., Ingerfeld, M., Anderson, N., Jackson, S.L., Moreno, S. "Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton" . Protoplasma, vol. 228, no. 4, 2006, pp. 189-199.
---------- VANCOUVER ----------
Pereyra, E., Ingerfeld, M., Anderson, N., Jackson, S.L., Moreno, S. Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton. Protoplasma. 2006;228(4):189-199.