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The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with 3.6 times more germ tubes per cell than control germinating sporangiospores. Disruption of the actin network with 2 μg of cytochalasin A per ml blocked the switch to tip growth; the effect was analogous to the one of 150 μM dibutyryl-cyclic AMP (cAMP), which we previously described to promote isodiametric growth via protein kinase A. 150 μM dibutyryl-cAMP antagonises partially the effect of 1 mM RGD; the cells still emit several germ tubes per mother cell but their number is smaller and the volume of the cell at germ tube emission is larger than with RGD alone. At higher concentrations the dibutyryl-cAMP overrides completely the effect of RGD. Our results suggest that M. rouxii has an RGD recognition system and demonstrate that RGD-containing peptides have a profound effect on the isotropic stage of growth and on the establishment of cell polarity and that cAMP analogues can override this effect.


Documento: Artículo
Título:RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
Autor:Pereyra, E.; Argimón, S.; Jackson, S.L.; Moreno, S.
Filiación:Depto. de Quím. Biol., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Dept. of Plant and Microbial Sci., University of Canterbury, Christchurch, New Zealand
Depto. de Quím. Biol., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Arg-Gly-Asp tripeptide; Cell morphology; Cyclic AMP; Mucor rouxii; ProNectin F; Actins; Cell Size; Cyclic AMP; Cytochalasins; Fibronectins; Mucor; Oligopeptides; Recombinant Proteins; Signal Transduction; Spores, Fungal; Amylomyces rouxii; Fungi; Mucor
Página de inicio:23
Página de fin:30
Título revista:Protoplasma
Título revista abreviado:Protoplasma
CAS:Actins; Cyclic AMP, 60-92-4; Cytochalasins; Fibronectins; Oligopeptides; ProNectin F, 137599-18-9; Recombinant Proteins; arginyl-glycyl-aspartic acid, 99896-85-2; cytochalasin A, 14110-64-6


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---------- APA ----------
Pereyra, E., Argimón, S., Jackson, S.L. & Moreno, S. (2003) . RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii. Protoplasma, 222(1-2), 23-30.
---------- CHICAGO ----------
Pereyra, E., Argimón, S., Jackson, S.L., Moreno, S. "RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii" . Protoplasma 222, no. 1-2 (2003) : 23-30.
---------- MLA ----------
Pereyra, E., Argimón, S., Jackson, S.L., Moreno, S. "RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii" . Protoplasma, vol. 222, no. 1-2, 2003, pp. 23-30.
---------- VANCOUVER ----------
Pereyra, E., Argimón, S., Jackson, S.L., Moreno, S. RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii. Protoplasma. 2003;222(1-2):23-30.