Abstract:
On-line tests with Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence assays. A mathematical model showed that 0.2 g/l is the best cell concentration to be used in the detection of low naphthalene concentrations, with the highest acceleration rate of bioluminescence sensitivity increase (0.46 nA l/(g min 2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3 μM) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16 μM), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA. © 2003 Elsevier Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | On-line detection of low naphthalene concentrations with a bioluminescent sensor |
Autor: | Valdman, E.; Valdman, B.; Battaglini, F.; Leite, S.G.F. |
Filiación: | INQUIMAE, Depto. de Quím. Inorg., Universidad de Buenos Aires, Buenos Aires, Argentina Escola de Química, Univ. Federal Do Rio de Janeiro, Centro de Tecnologia, Bloco E, 21941-900, Rio de Janeiro, Brazil
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Palabras clave: | Bioluminescence; Naphthalene; On-line detection; Ps. fluorescens HK44; Pseudomonas; Pseudomonas fluorescens |
Año: | 2004
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Volumen: | 39
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Número: | 10
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Página de inicio: | 1217
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Página de fin: | 1222
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DOI: |
http://dx.doi.org/10.1016/S0032-9592(03)00248-6 |
Título revista: | Process Biochemistry
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Título revista abreviado: | Process Biochem.
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ISSN: | 00329592
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CODEN: | PBCHE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00329592_v39_n10_p1217_Valdman |
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Citas:
---------- APA ----------
Valdman, E., Valdman, B., Battaglini, F. & Leite, S.G.F.
(2004)
. On-line detection of low naphthalene concentrations with a bioluminescent sensor. Process Biochemistry, 39(10), 1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6---------- CHICAGO ----------
Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F.
"On-line detection of low naphthalene concentrations with a bioluminescent sensor"
. Process Biochemistry 39, no. 10
(2004) : 1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6---------- MLA ----------
Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F.
"On-line detection of low naphthalene concentrations with a bioluminescent sensor"
. Process Biochemistry, vol. 39, no. 10, 2004, pp. 1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6---------- VANCOUVER ----------
Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F. On-line detection of low naphthalene concentrations with a bioluminescent sensor. Process Biochem. 2004;39(10):1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6