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Abstract:

On-line tests with Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence assays. A mathematical model showed that 0.2 g/l is the best cell concentration to be used in the detection of low naphthalene concentrations, with the highest acceleration rate of bioluminescence sensitivity increase (0.46 nA l/(g min 2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3 μM) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16 μM), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA. © 2003 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:On-line detection of low naphthalene concentrations with a bioluminescent sensor
Autor:Valdman, E.; Valdman, B.; Battaglini, F.; Leite, S.G.F.
Filiación:INQUIMAE, Depto. de Quím. Inorg., Universidad de Buenos Aires, Buenos Aires, Argentina
Escola de Química, Univ. Federal Do Rio de Janeiro, Centro de Tecnologia, Bloco E, 21941-900, Rio de Janeiro, Brazil
Palabras clave:Bioluminescence; Naphthalene; On-line detection; Ps. fluorescens HK44; Pseudomonas; Pseudomonas fluorescens
Año:2004
Volumen:39
Número:10
Página de inicio:1217
Página de fin:1222
DOI: http://dx.doi.org/10.1016/S0032-9592(03)00248-6
Título revista:Process Biochemistry
Título revista abreviado:Process Biochem.
ISSN:00329592
CODEN:PBCHE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00329592_v39_n10_p1217_Valdman

Referencias:

  • Lee, H.J., Villaume, J., Cullen, D.C., Kim, B.C., Gu, M.B., Monitoring and classification of PAH toxicity using an immobilized bioluminescent bacteria (2003) Biosens. Bioelectron., 18, pp. 571-577
  • Contaminant Candidate List Preliminary Regulatory Determination Support Document for Napthalene, p. 2002. , Washington, DC
  • Langwaldt, J.H., Puhakka, J.A., On-site biological remediation of contanimated groundwater: A review (2000) Environ. Pollut., 107, pp. 187-197
  • Reshetilov, A.N., Iliasov, P.V., Filonov, A.E., Gayazov, R.R., Kosheleva, I.A., Boronin, A.M., Pseudomonas putida as a receptor element of microbial sensor for naphthalene detection (1997) Process Biochem., 32, pp. 487-493
  • Kelly, C.J., Lajoie, C.A., Layton, A.C., Sayler, G.S., Bioluminescent reporter bacterium for toxicity monitoring in biological wastewater treatment systems (1999) Water Environ. Res., 71, pp. 31-35
  • Bechor, O., Smulski, D.R., Van Dyk, T.K., Larossa, R.A., Belkin, S., Recombinant microorganisms as environmental biosensor: Pollutants detection by Escherichia coli bearing fabA′lux fusions (2002) J. Biotechnol., 94, pp. 125-132
  • Choi, S.H., Gu, M.B., A portable toxicity biosensor using freeze-dried recombinant bioluminescent bacteria (2002) Biosens. Bioelectron., 17, pp. 433-440
  • Simpson, M.L., Sayler, G.S., Applegate, B.M., Ripp, S., Nivens, D.E., Paulus, M.J., Bioluminescent-bioreporter integrated circuits from novel whole-cell biosensors (1998) Trends Biotechnol., 16, pp. 332-338
  • Lewis, J.C., Feltus, A., Ensor, C.M., Ramanathan, S., Daunert, S., Applications of reporter genes (1998) Anal. Chem., 1, pp. 579A-585A
  • Applegate, B.M., Kebrmeyer, S.R., Sayler, G.S., A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene ethybenzene and xylene (BTEX) sensing (1998) Appl. Environ. Microbiol., 64, pp. 2730-2735
  • Neilson, J.W., Pierce, S.A., Maier, R.M., Factors influencing expression of luxCDABE and nah genes in Pseudomonas putida RB1353(NAH7, pUTk9) in dynamic systems (1999) Appl. Environ. Microbiol., 65, pp. 3473-3482
  • Purohit, H.J., Biosensors as molecular tools for use in bioremediation (2003) J. Clean. Prod., 11, pp. 293-301
  • Filonov, A.E., Karpov, A.V., Kosheleva, I.A., Puntus, I.F., Balashova, N.V., Boronin, A.M., The efficiency of salicylate utilization by Pseudomonas putida strains catabolizing naphthalene via different biochemical pathways (2000) Process Biochem., 35, pp. 983-987
  • Garbiso, C., Alkorta, I., Utilization of genetically engineered microorganisms (GEMs) for bioremediation (1999) J. Chem. Technol. Biotechnol., 74, pp. 599-606
  • Ripp, S., Nivens, D.E., Ahn, Y., Werner, C., Jarrel, I.V.J., Easter, J.P., Controlled filed release of bioluminescence genetically engineered microorganism for bioremediation process monitoring and control (2000) Environ. Sci. Technol., 34, pp. 846-853
  • Uesugi, S.L., Yarwood, R.R., Selker, J.S., Bottomley, P.J., A model that uses the induction phase of lux gene-dependent bioluminescence in Pseudomonas fluorescens HK44 to quantify cell density in translucent porous media (2001) J. Microbiol. Methods, 47, pp. 315-322
  • King, J.M.H., Digrazia, P.M., Applegate, B., Burlage, R., Sanseverino, J., Dunbar, P., Rapid, sensitive bioluminescence reporter technology for naphthalene exposure and biodegradation (1990) Science, 249, pp. 778-781
  • Valdman, E., Valdman, B., Leite, S.G.F., Battaglini, F., Determinación de naftaleno por bioluminiscencia (2001) Proceedings of the I Jornadas Nacionales AAQA, , Rosário, Argentina, 6-7 December
  • Heitzer, A., Malachowsky, K., Thonnard, J.E., Bienkowski, P.R., White, D.C., Sayler, G.S., Optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium (1994) Appl. Environ. Microbiol., 60, pp. 1487-1494
  • Willardson, B.M., Wilkins, J.F., Rand, T.A., Schupp, J.M., Hill, K.K., Keim, P., Development and testing of a bacterial biosensor for toluene-based environmental contaminants (1998) Appl. Environ. Microbiol., 64, pp. 1006-1012
  • Helm, S., Schieder, I., Binz, D., Vogel, A., Bilitewski, U., Development of an automated microbial sensor system (1999) Biosens. Bioelectron., 14, pp. 187-193

Citas:

---------- APA ----------
Valdman, E., Valdman, B., Battaglini, F. & Leite, S.G.F. (2004) . On-line detection of low naphthalene concentrations with a bioluminescent sensor. Process Biochemistry, 39(10), 1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6
---------- CHICAGO ----------
Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F. "On-line detection of low naphthalene concentrations with a bioluminescent sensor" . Process Biochemistry 39, no. 10 (2004) : 1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6
---------- MLA ----------
Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F. "On-line detection of low naphthalene concentrations with a bioluminescent sensor" . Process Biochemistry, vol. 39, no. 10, 2004, pp. 1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6
---------- VANCOUVER ----------
Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F. On-line detection of low naphthalene concentrations with a bioluminescent sensor. Process Biochem. 2004;39(10):1217-1222.
http://dx.doi.org/10.1016/S0032-9592(03)00248-6