Abstract:
The partially cyclized μ/v-carrageenan 1C3, isolated from the red seaweed Gigartina skottsbergii, was previously shown to be a potent inhibitor of the in vitro replication of Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Here the protective effect of 1C3 in a murine model of intraperitoneal (i.p.) HSV-1 infection was evaluated. OF1 mice were i.p. infected with 5 × 105 PFU of HSV-1 KOS strain, and the effects of different treatments with 1C3 were studied. When 30 mg/kg of body weight of 1C3 was administered by the i.p. route immediately after HSV-1 infection, 87.5% survival of the animals was achieved (p < 0.005), associated with a delay in the mean day of death in 1C3-treated non-surviving mice. Animal survival was not improved when multiple doses of 1C3 were also given in the period 1-48 h post-infection, and no protection was afforded when treatment was started after 24 h of infection. When virus and compound were injected by different routes, i.p. and intravenous (i.v.), respectively, a still significant protection was achieved (40% survival, p < 0.05). No toxicity of 1C3 for the animals was recorded. The pharmacokinetic properties were analyzed after injection of 1C3 into the tail vein by monitoring of [3H]-1C3 in plasma and organs and by a bioassay of the anti-HSV-1 activity remaining in serum after non-radioactive 1C3 inoculation. A very rapid disappearance of the compound from the blood was observed since only 5.9-0.9% of the radioactivity of the initially administered [3H]-1C3 appeared in the plasma between 5-300 minutes after administration. A transient peak of radioactivity was detected in the kidney 15 minutes after inoculation. The bioassay confirms the presence of the compound circulating in a biologically active form up to 1 hour after injection. © Georg Thieme Verlag KG Stuttgart.
Registro:
Documento: |
Artículo
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Título: | Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
Autor: | Pujol, C.A.; Scolaro, L.A.; Ciancia, M.; Matulewicz, M.C.; Cerezo, A.S.; Damonte, E.B. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428BGA Buenos Aires, Argentina
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Palabras clave: | Antiviral agent; Gigartina skottsbergii; Gigartinaceae; Gigartinales; Herpes simplex virus; Mouse intraperitoneal infection; Natural carrageenan; Pharmacokinetics; carrageenan; Gigartina skottsbergii extract; plant extract; unclassified drug; animal model; antiviral activity; article; body weight; controlled study; dose response; drug bioavailability; drug blood level; drug half life; drug mechanism; herpes simplex; Herpes simplex virus 1; Herpes simplex virus 2; mouse; nonhuman; survival; Algae, Red; Animals; Antiviral Agents; Carrageenan; Disease Models, Animal; Herpes Simplex; Injections, Intraperitoneal; Male; Mice; Phytotherapy; Plant Preparations; Tissue Distribution; algae; Animalia; Gigartina skottsbergii; Gigartinaceae; Gigartinales; Herpes; Human herpesvirus 1; Human herpesvirus 2; Murinae; Simplexvirus |
Año: | 2006
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Volumen: | 72
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Número: | 2
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Página de inicio: | 121
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Página de fin: | 125
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DOI: |
http://dx.doi.org/10.1055/s-2005-373168 |
Título revista: | Planta Medica
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Título revista abreviado: | Planta Med.
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ISSN: | 00320943
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CODEN: | PLMEA
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CAS: | carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; Antiviral Agents; Carrageenan, 9000-07-1; Plant Preparations
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v72_n2_p121_Pujol |
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Citas:
---------- APA ----------
Pujol, C.A., Scolaro, L.A., Ciancia, M., Matulewicz, M.C., Cerezo, A.S. & Damonte, E.B.
(2006)
. Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection. Planta Medica, 72(2), 121-125.
http://dx.doi.org/10.1055/s-2005-373168---------- CHICAGO ----------
Pujol, C.A., Scolaro, L.A., Ciancia, M., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B.
"Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection"
. Planta Medica 72, no. 2
(2006) : 121-125.
http://dx.doi.org/10.1055/s-2005-373168---------- MLA ----------
Pujol, C.A., Scolaro, L.A., Ciancia, M., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B.
"Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection"
. Planta Medica, vol. 72, no. 2, 2006, pp. 121-125.
http://dx.doi.org/10.1055/s-2005-373168---------- VANCOUVER ----------
Pujol, C.A., Scolaro, L.A., Ciancia, M., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B. Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection. Planta Med. 2006;72(2):121-125.
http://dx.doi.org/10.1055/s-2005-373168