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Abstract:

Four macrocyclic trichothecenes, roridin A, roridin E, verrucarin A and verrucarin J, produced by the hypocrealean epibiont of Baccharis coridifolia, were evaluated for their inhibitory activity against the arenavirus Junin (JUNV), the etiological agent of Argentine hemorrhagic fever. The trichothecenes achieved a dose-dependent inhibition of JUNV multiplication at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) values determined by a virus yield inhibition assay were in the range 1.2-4.9 ng/ml. The most active compound was verrucarin J which reduced JUNV yield more than 2 log units and had a similar effect against the arenavirus Tacaribe. The trichothecenes lacked virucidal effects on JUNV virions. From time of addition and removal experiments, it can be concluded that verrucarin J inhibited a late stage in the replicative cycle of JUNV, after 5 h of adsorption.

Registro:

Documento: Artículo
Título:Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
Autor:García, C.C.; Rosso, M.L.; Bertoni, M.D.; Maier, M.S.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2 Piso 4, 1428 Buenos Aires, Argentina
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Orgänica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Antiviral activity; Arenaviruses; Asteraceae; Baccharis coridifolia; Junin virus; Trichothecenes; antivirus agent; macrocyclic compound; roridin A; roridin e; sesquiterpenoid; unclassified drug; verrucarin A; verrucarin j; animal cell; antiviral activity; Arenavirus; article; baccharis coridifolia; cell viability; concentration response; controlled study; dose response; drug screening; fungus; hemorrhagic fever; IC 50; Junin virus; nonhuman; virion; virus adsorption; virus inhibition; Animals; Antiviral Agents; Asteraceae; Cercopithecus aethiops; Dose-Response Relationship, Drug; Junin virus; Plant Extracts; Trichothecenes; Vero Cells; Animalia; Arenavirus; Argentina (fish); Asteraceae; Baccharis; Fungi; Hypocreales; Junin virus
Año:2002
Volumen:68
Número:3
Página de inicio:209
Página de fin:212
DOI: http://dx.doi.org/10.1055/s-2002-23134
Título revista:Planta Medica
Título revista abreviado:Planta Med.
ISSN:00320943
CODEN:PLMEA
CAS:roridin A, 14729-29-4; verrucarin A, 3148-09-2; verrucarin j, 4643-58-7; Antiviral Agents; Plant Extracts; Trichothecenes; muconomycin A, 3148-09-2; roridin A, 14729-29-4; roridin E, 16891-85-3; verrucarin J, 4643-58-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v68_n3_p209_Garcia

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Citas:

---------- APA ----------
García, C.C., Rosso, M.L., Bertoni, M.D., Maier, M.S. & Damonte, E.B. (2002) . Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia. Planta Medica, 68(3), 209-212.
http://dx.doi.org/10.1055/s-2002-23134
---------- CHICAGO ----------
García, C.C., Rosso, M.L., Bertoni, M.D., Maier, M.S., Damonte, E.B. "Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia" . Planta Medica 68, no. 3 (2002) : 209-212.
http://dx.doi.org/10.1055/s-2002-23134
---------- MLA ----------
García, C.C., Rosso, M.L., Bertoni, M.D., Maier, M.S., Damonte, E.B. "Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia" . Planta Medica, vol. 68, no. 3, 2002, pp. 209-212.
http://dx.doi.org/10.1055/s-2002-23134
---------- VANCOUVER ----------
García, C.C., Rosso, M.L., Bertoni, M.D., Maier, M.S., Damonte, E.B. Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia. Planta Med. 2002;68(3):209-212.
http://dx.doi.org/10.1055/s-2002-23134