Abstract:
The bioactivity-guided fractionation of an active chloroform extract of Conyza albida led to the isolation of three alkenynes, deca-4,6-diyn-2-(Z)-enoic methyl ester (1), deca-4,6-diyn-2-(Z)-enoic ethyl ester (2) and deca-2,4-diene-4-hydroxy-6-yn-l,4-olide (3), and the terpenoid spathulenol (4), as the active toxic metabolites in the Artemia sp. lethality test. When tested in the KB cell cytotoxicity assay, compounds 1-4 demonstrated 1C 50 values of 52.2, 38.4, 117.9, and 83.8 μM, respectively. All compounds studied were inactive in the DNA methyl green and DNA strand scission assays, while compounds 3 and 4 showed moderate activity as inhibitors of human topoisomerase I. Compound 2 is reported here for the first time.
Registro:
Documento: |
Artículo
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Título: | Bioactive constituents of Conyza albida |
Autor: | Del Pacciaroni, A.V.; Mongelli, E.; Espinar, L.A.; Romano, A.; Ciccia, G.; Silva, G.L. |
Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Quím. U.N.C., IMBIV-CONICET, Pabellón de Ciencias II, Ciudad Universitaria, 5000 Córdoba, Argentina Cátedra de Microbiología Industrial y Biotecnología, IQUIMEFA-CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina Cátedra de Farmacognosia, IQUIMEFA-CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
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Idioma: |
Inglés
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Palabras clave: | Alkenynes; Compositae; Conyza albida; Cytotoxic activity; DNA interaction; DNA strand scission; Topoisomerase 1; chloroform; deca 2,4 diene 4 hydroxy 6 yn 1,4 olide; deca 4,6 diyn 2 enoic ethyl ester; deca 4,6 diyn 2 enoic methyl ester; DNA; DNA topoisomerase; methyl green; plant extract; spathulenol; unclassified drug; animal experiment; article; controlled study; cytotoxicity; drug activity; fractionation; human; human cell; medicinal plant; nonhuman; plant leaf; shrimp; Asteraceae; Humans; Molecular Structure; Plant Extracts; Spectrum Analysis |
Año: | 2000
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Volumen: | 66
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Número: | 8
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Página de inicio: | 720
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Página de fin: | 723
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DOI: |
http://dx.doi.org/10.1055/s-2000-9600 |
Título revista: | Planta Medica
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Título revista abreviado: | Planta Med.
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ISSN: | 00320943
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CODEN: | PLMEA
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CAS: | DNA, 9007-49-2; DNA topoisomerase, 80449-01-0; chloroform, 67-66-3; methyl green, 54327-10-5; spathulenol, 6750-60-3; Plant Extracts
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v66_n8_p720_DelPacciaroni |
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Citas:
---------- APA ----------
Del Pacciaroni, A.V., Mongelli, E., Espinar, L.A., Romano, A., Ciccia, G. & Silva, G.L.
(2000)
. Bioactive constituents of Conyza albida. Planta Medica, 66(8), 720-723.
http://dx.doi.org/10.1055/s-2000-9600---------- CHICAGO ----------
Del Pacciaroni, A.V., Mongelli, E., Espinar, L.A., Romano, A., Ciccia, G., Silva, G.L.
"Bioactive constituents of Conyza albida"
. Planta Medica 66, no. 8
(2000) : 720-723.
http://dx.doi.org/10.1055/s-2000-9600---------- MLA ----------
Del Pacciaroni, A.V., Mongelli, E., Espinar, L.A., Romano, A., Ciccia, G., Silva, G.L.
"Bioactive constituents of Conyza albida"
. Planta Medica, vol. 66, no. 8, 2000, pp. 720-723.
http://dx.doi.org/10.1055/s-2000-9600---------- VANCOUVER ----------
Del Pacciaroni, A.V., Mongelli, E., Espinar, L.A., Romano, A., Ciccia, G., Silva, G.L. Bioactive constituents of Conyza albida. Planta Med. 2000;66(8):720-723.
http://dx.doi.org/10.1055/s-2000-9600