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Abstract:

Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutamines, which presents myristoylation and palmitoylation consensus sites and a PEST sequence indicative of rapid protein degradation. StCDPK3 gene (circa 11 kb) is localized in chromosome 3, shares the eight exons and seven introns structure with other isoforms from subgroup IIa and contains an additional intron in the 5′UTR region. StCDPK3 expression is ubiquitous being transcripts more abundant in early elongating stolons (ES), leaves and roots, however isoform specific antibodies only detected the protein in leaf particulate extracts. The recombinant 6xHis-StCDPK3 is an active kinase that differs in its kinetic parameters and calcium requirements from StCDPK1 and 2 isoforms. In vitro, StCDPK3 undergoes autophosphorylation regardless of the addition of calcium. The StCDPK3 promoter region (circa 1,800 bp) was subcloned by genome walking and fused to GUS. Light and ABRE responsive elements were identified in the promoter region as well as elements associated to expression in roots. StCDPK3 expression was enhanced by ABA while GA decreased it. Potato transgenic lines harboring StCDPK3 promoter{proportion}GUS construct were generated by Agrobacterium tumefaciens mediated plant transformation. Promoter activity was detected in leaves, root tips and branching points, early ES, tuber eyes and developing sprouts indicating that StCDPK3 is expressed in actively growing organs. © 2012 Springer-Verlag.

Registro:

Documento: Artículo
Título:The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
Autor:Grandellis, C.; Giammaria, V.; Bialer, M.; Santin, F.; Lin, T.; Hannapel, D.J.; Ulloa, R.M.
Filiación:Inst. de Investigaciones en Ingeniería Genetica y Biologia Molecular Dr. Hector N. Torres, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490 2 piso, C1428ADN Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Plant Biology Major, 253 Horticulture Hall, Iowa State University, Ames, IA, 50011-1100, United States
Palabras clave:Calcium dependent protein kinases; Phytohormone responses; Signaling; Solanum tuberosum; calcium protein kinase; calcium-dependent protein kinase; hybrid protein; isoprotein; plant RNA; protein kinase; vegetable protein; amino acid sequence; antibody specificity; article; DNA sequence; enzymology; gene expression regulation; genetics; growth, development and aging; metabolism; molecular genetics; nucleotide sequence; phosphorylation; phylogeny; physiological stress; physiology; plant leaf; plant tuber; potato; promoter region; sequence alignment; signal transduction; transgenic plant; Amino Acid Sequence; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Molecular Sequence Data; Organ Specificity; Phosphorylation; Phylogeny; Plant Leaves; Plant Proteins; Plant Tubers; Plants, Genetically Modified; Promoter Regions, Genetic; Protein Isoforms; Protein Kinases; Recombinant Fusion Proteins; RNA, Plant; Sequence Alignment; Sequence Analysis, DNA; Signal Transduction; Solanum tuberosum; Stress, Physiological; Agrobacterium tumefaciens; Solanum tuberosum
Año:2012
Volumen:236
Número:6
Página de inicio:1831
Página de fin:1848
DOI: http://dx.doi.org/10.1007/s00425-012-1732-9
Título revista:Planta
Título revista abreviado:Planta
ISSN:00320935
CODEN:PLANA
CAS:protein kinase, 9026-43-1; Plant Proteins; Protein Isoforms; Protein Kinases, 2.7.-; RNA, Plant; Recombinant Fusion Proteins; calcium-dependent protein kinase, 2.7.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v236_n6_p1831_Grandellis

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Citas:

---------- APA ----------
Grandellis, C., Giammaria, V., Bialer, M., Santin, F., Lin, T., Hannapel, D.J. & Ulloa, R.M. (2012) . The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs. Planta, 236(6), 1831-1848.
http://dx.doi.org/10.1007/s00425-012-1732-9
---------- CHICAGO ----------
Grandellis, C., Giammaria, V., Bialer, M., Santin, F., Lin, T., Hannapel, D.J., et al. "The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs" . Planta 236, no. 6 (2012) : 1831-1848.
http://dx.doi.org/10.1007/s00425-012-1732-9
---------- MLA ----------
Grandellis, C., Giammaria, V., Bialer, M., Santin, F., Lin, T., Hannapel, D.J., et al. "The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs" . Planta, vol. 236, no. 6, 2012, pp. 1831-1848.
http://dx.doi.org/10.1007/s00425-012-1732-9
---------- VANCOUVER ----------
Grandellis, C., Giammaria, V., Bialer, M., Santin, F., Lin, T., Hannapel, D.J., et al. The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs. Planta. 2012;236(6):1831-1848.
http://dx.doi.org/10.1007/s00425-012-1732-9