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Abstract:

Plastid genome transformation offers an attractive methodology for transgene expression in plants, but for potato, only expression of gfp transgene (besides the selective gene aadA) has been published. We report here successful expression of β-glucuronidase in transplastomic Solanum tuberosum (var. Desiree) plants, with accumulation levels for the recombinant protein of up to 41% of total soluble protein in mature leaves. To our knowledge, this is the highest expression level reported for a heterologous protein in S. tuberosum. Accumulation of the recombinant protein in soil-grown minitubers was very low, as described in previous reports. Interestingly, microtubers developed in vitro showed higher accumulation of β-glucuronidase. As light exposure during their development could be the trigger for this high accumulation, we analyzed the effect of light on β-glucuronidase accumulation in transplastomic tubers. Exposure to light for 8 days increased β-glucuronidase accumulation in soil-grown tubers, acting as a light-inducible expression system for recombinant protein accumulation in tuber plastids. In this paper we show that plastid transformation in potato allows the highest recombinant protein accumulation in foliar tissue described so far for this food crop. We also demonstrate that in tubers high accumulation is possible and depends on light exposure. Because tubers have many advantages as protein storage organs, these results could lead to new recombinant protein production schemes based on potato. © 2011 Springer-Verlag.

Registro:

Documento: Artículo
Título:Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro
Autor:Segretin, M.E.; Lentz, E.M.; Wirth, S.A.; Morgenfeld, M.M.; Bravo-Almonacid, F.F.
Filiación:Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingenieria Genetica y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do. Piso, C1428ADN Ciudad Autónoma de Buenos Aires, Argentina
Laboratorio de Agrobiotecnología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA Ciudad Autónoma de Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, Intendente Guiraldes 2160 Pab. II, Ciudad Universitaria, C1428EGA Ciudad Autónoma de Buenos Aires, Argentina
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina
Palabras clave:β-Glucuronidase; Chloroplast; Plastid transformation; Potato; Tobacco; Tuber; beta glucuronidase; recombinant protein; vegetable protein; article; biosynthesis; comparative study; enzymology; gene expression regulation; genetics; plant leaf; plant tuber; plastid; potato; tobacco; transgene; transgenic plant; Gene Expression Regulation, Plant; Glucuronidase; Plant Leaves; Plant Proteins; Plant Tubers; Plants, Genetically Modified; Plastids; Recombinant Proteins; Solanum tuberosum; Tobacco; Transgenes; Nicotiana tabacum; Solanum tuberosum; Tuber (truffle)
Año:2012
Volumen:235
Número:4
Página de inicio:807
Página de fin:818
DOI: http://dx.doi.org/10.1007/s00425-011-1541-6
Título revista:Planta
Título revista abreviado:Planta
ISSN:00320935
CODEN:PLANA
CAS:beta glucuronidase, 9001-45-0; Glucuronidase, 3.2.1.31; Plant Proteins; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v235_n4_p807_Segretin

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Citas:

---------- APA ----------
Segretin, M.E., Lentz, E.M., Wirth, S.A., Morgenfeld, M.M. & Bravo-Almonacid, F.F. (2012) . Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro. Planta, 235(4), 807-818.
http://dx.doi.org/10.1007/s00425-011-1541-6
---------- CHICAGO ----------
Segretin, M.E., Lentz, E.M., Wirth, S.A., Morgenfeld, M.M., Bravo-Almonacid, F.F. "Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro" . Planta 235, no. 4 (2012) : 807-818.
http://dx.doi.org/10.1007/s00425-011-1541-6
---------- MLA ----------
Segretin, M.E., Lentz, E.M., Wirth, S.A., Morgenfeld, M.M., Bravo-Almonacid, F.F. "Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro" . Planta, vol. 235, no. 4, 2012, pp. 807-818.
http://dx.doi.org/10.1007/s00425-011-1541-6
---------- VANCOUVER ----------
Segretin, M.E., Lentz, E.M., Wirth, S.A., Morgenfeld, M.M., Bravo-Almonacid, F.F. Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro. Planta. 2012;235(4):807-818.
http://dx.doi.org/10.1007/s00425-011-1541-6