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Abstract:

ABF/AREB bZIP transcription factors mediate plant abiotic stress responses by regulating the expression of stress-related genes. These proteins bind to the abscisic acid (ABA)-responsive element (ABRE), which is the major cis-acting regulatory sequence in ABA-dependent gene expression. In an effort to understand the molecular mechanisms of abiotic stress resistance in cultivated potato (Solanum tuberosum L.), we have cloned and characterized an ABF/AREB-like transcription factor from potato, named StABF1. The predicted protein shares 45-57% identity with A. thaliana ABFs proteins and 96% identity with the S. lycopersicum SlAREB1 and presents all of the distinctive features of ABF/AREB transcription factors. Furthermore, StABF1 is able to bind to the ABRE in vitro. StABF1 gene is induced in response to ABA, drought, salt stress and cold, suggesting that it might be a key regulator of ABA-dependent stress signaling pathways in cultivated potato. StABF1 is phosphorylated in response to ABA and salt stress in a calcium-dependent manner, and we have identified a potato CDPK isoform (StCDPK2) that phosphorylates StABF1 in vitro. Interestingly, StABF1 expression is increased during tuber development and by tuber-inducing conditions (high sucrose/nitrogen ratio) in leaves. We also found that StABF1 calcium-dependent phosphorylation is stimulated by tuber-inducing conditions and inhibited by gibberellic acid, which inhibits tuberization. © 2011 Springer-Verlag.

Registro:

Documento: Artículo
Título:Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro
Autor:García, M.N.M.; Giammaria, V.; Grandellis, C.; Téllez-Iñón, M.T.; Ulloa, R.M.; Capiati, D.A.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 2 piso, C1428ADN Buenos Aires, Argentina
Palabras clave:Abiotic stress; Abscisic acid; Solanum tuberosum L.; StABF1; StCDPK2; Tuberization; abscisic acid; basic leucine zipper transcription factor; vegetable protein; Arabidopsis; article; comparative study; gene expression regulation; genetics; growth, development and aging; metabolism; phosphorylation; physiological stress; physiology; plant tuber; Solanum; Abscisic Acid; Arabidopsis; Basic-Leucine Zipper Transcription Factors; Gene Expression Regulation, Plant; Phosphorylation; Plant Proteins; Plant Tubers; Solanum; Stress, Physiological; Arabidopsis thaliana; Lycopersicon; Solanum tuberosum; Tuber (truffle)
Año:2012
Volumen:235
Número:4
Página de inicio:761
Página de fin:778
DOI: http://dx.doi.org/10.1007/s00425-011-1540-7
Título revista:Planta
Título revista abreviado:Planta
ISSN:00320935
CODEN:PLANA
CAS:abscisic acid, 21293-29-8; Abscisic Acid, 21293-29-8; Basic-Leucine Zipper Transcription Factors; Plant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v235_n4_p761_Garcia

Referencias:

  • Abelenda, J.A., Navarro, C., Prat, S., From the model to the crop: genes controlling tuber formation in potato (2011) Curr Opin Biotechnol, 22, pp. 287-292
  • Banerjee, A.K., Lin, T., Hannapel, D.J., Untranslated regions of a mobile transcript mediate RNA metabolism (2009) Plant Physiol, 151, pp. 1831-1843
  • Bhatnagar-Mathur, P., Vadez, V., Sharma, K.K., Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects (2008) Plant Cell Rep, 27, pp. 411-424
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Casaretto, J.A., Ho, T.-H., Transcriptional regulation by abscisic acid in barley (Hordeum vulgare L.) seeds involves autoregulation of the transcription factor HvABI5 (2005) Plant Mol Biol, 57, pp. 21-34
  • Century, K., Reuber, T.L., Ratcliffe, O.J., Regulating the regulators: the future prospects for transcription-factor-based agricultural biotechnology products (2008) Plant Physiol, 147, pp. 20-29
  • Chico, J.M., Raíces, M., Téllez-Iñón, M.T., Ulloa, R.M., A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants (2002) Plant Physiol, 128, pp. 256-270
  • Choi, H., Hong, J., Ha, J., Kang, J., Kim, S.Y., ABFs, a family of ABA-responsive element binding factors (2000) J Biol Chem, 275, pp. 1723-1730
  • Choi, H.I., Park, H.J., Park, J.H., Kim, S., Im, M.Y., Seo, H.H., Kim, Y.W., Kim, S.Y., Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity (2005) Plant Physiol, 139, pp. 1750-1761
  • Fan, L.M., Zhao, Z.X., Assmann, S.M., Guard cells: a dynamic signaling model (2004) Curr Opin Plant Biol, 7, pp. 537-546
  • Fischer, L., Lipavska, H., Hausman, J.F., Opatrny, Z., Morphological and molecular characterization of a spontaneously tuberizing potato mutant: an insight into the regulatory mechanisms of tuber induction (2008) BMC Plant Biol, 8, p. 117
  • Fujii, H., Zhu, J.K., Arabidopsis mutant deficient in 3 abscisic acid activated protein kinases reveals critical roles in growth, reproduction, and stress (2009) Proc Natl Acad Sci USA, 106, pp. 8380-8385
  • Fujita, Y., Fujita, M., Satoh, R., Maruyama, K., Parvez, M.M., Seki, M., Hiratsu, K., Yamaguchi-Shinosaki, K., AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis (2005) Plant Cell, 17, pp. 3470-3488
  • Fujita, Y., Fujita, M., Shinozaki, K., Yamaguchi-Shinozaki, K., ABA-mediated transcriptional regulation in response to osmotic stress in plants (2011) J Plant Res, 124, pp. 509-525
  • Furihata, T., Maruyama, K., Fujita, Y., Umezawa, T., Yoshida, R., Shinozaki, K., Yamaguchi-Shinozaki, K., Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1 (2006) Proc Natl Acad Sci USA, 103, pp. 1988-1993
  • Giammaria, V., Grandellis, C., Bachmann, S., Gargantini, P.R., Feingold, S.E., Bryan, G., Ulloa, R.M., StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling (2011) Planta, 233, pp. 593-609
  • Hannapel, D.J., Differential expression of potato tuber protein genes (1990) Plant Physiol, 94, pp. 919-925
  • Harmon, A.C., Calcium-regulated protein kinases of plants (2003) Gravit Space Biol Bull, 16, pp. 83-90
  • Hebsgaard, S.M., Korning, P.G., Tolstrup, N., Engelbrecht, J., Rouze, P., Brunak, S., Splice site prediction in Arabidopsis thaliana DNA by combining local and global sequence information (1996) Nucleic Acids Res, 24, pp. 3439-3452
  • Himmelbach, A., Yang, Y., Grill, E., Relay and control of abscisic acid signaling (2003) Curr Opin Plant Biol, 6, pp. 470-479
  • Hirayama, T., Shinozaki, K., Research on plant abiotic stress responses in the post-genome era: past, present and future (2010) Plant J, 61, pp. 1041-1052
  • Hossain, M.A., Cho, J.I., Han, M., Ahn, C.H., Jeon, J.S., An, G., Park, P.B., The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice (2010) J Plant Physiol, 167, pp. 1512-1520
  • Hossain, M.A., Lee, Y., Cho, J.I., Ahn, C.H., Lee, S.K., Jeon, J.S., Kang, H., Park, P.B., The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice (2010) Plant Mol Biol, 72, pp. 557-566
  • Hsieh, T.H., Li, C.W., Su, R.C., Cheng, C.P., Sanjaya, Tsai, Y.C., Chan, M.T., A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response (2010) Planta, 231, pp. 1459-1473
  • Hu, X.Y., Neill, S.J., Cai, W.M., Tang, Z.C., Induction of defence gene expression by oligogalacturonic acid requires increases in both cytosolic calcium and hydrogen peroxide in Arabidopsis thaliana (2004) Cell Res, 14, pp. 234-240
  • Jakoby, M., Weisshaar, B., Dröge-Laser, W., Vicente-Carbajosa, J., Tiedemann, J., Kroj, T., Parcy, F., bZIP transcription factors in Arabidopsis (2002) Trends Plant Sci, 7, pp. 106-111. , bZIP Research Group
  • Kang, J., Choi, H., Im, M., Kim, S.Y., Arabidopsis basic leucine zipper proteins mediate stress-responsive abscisic acid signaling (2002) Plant Cell, 14, pp. 343-357
  • Kaplan, B., Davydov, O., Knight, H., Galon, Y., Knight, M.R., Fluhr, R., Fromm, H., Rapid transcriptome changes induced by cytosolic Ca 2+ transients reveal ABRE-related sequences as Ca 2+-responsive cis elements in Arabidopsis (2006) Plant Cell, 18, pp. 2733-2748
  • Kim, S., Kang, J.Y., Cho, D.I., Park, J.H., Kim, S.Y., ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance (2004) Plant J, 40, pp. 75-87
  • Kirchler, T., Briesemeister, S., Singer, M., Schütze, K., Keinath, M., Kohlbacher, O., Vicente-Carbajosa, J., Chaban, C., The role of phosphorylatable serine residues in the DNA-binding domain of Arabidopsis bZIP transcription factors (2010) Eur J Cell Biol, 89, pp. 175-183
  • Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N., Yoshioka, H., Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase (2007) Plant Cell, 19, pp. 1065-1080
  • Kobayashi, F., Maeta, E., Terashima, A., Takumi, S., Positive role of a wheat HvABI5 ortholog in abiotic stress response of seedlings (2008) Physiol Plant, 134, pp. 74-86
  • Lecourieux, D., Ranjeva, R., Pugin, A., Calcium in plant defence-signalling pathways (2006) New Phytol, 171, pp. 249-269
  • Li, Z., Komatsu, S., Molecular cloning and characterization of calreticulin, a calcium-binding protein involved in the regeneration of rice cultured suspension cells (2000) Eur J Biochem, 267, pp. 737-745
  • Lorenzen, J.H., Ewing, E.E., Starch accumulation in leaves of potato (Solanum tuberosum L.) during the 1st 18 days of photoperiod treatment (1992) Ann Bot, 69, pp. 481-485
  • McAinsh, M.R., Pittman, J.K., Shaping the calcium signature (2009) New Phytol, 181, pp. 275-294
  • Menzel, B.M., Tuberization in potato (Solanum tuberosum) cultivar Sebago at high temperatures: responses to gibberellins and growth inhibitors (1980) Ann Bot, 46, pp. 259-266
  • Mori, I.C., Murata, Y., Yang, Y., Munemasa, S., Wang, Y.F., Andreoli, S., Tiriac, H., Schroeder, J.I., CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca 2+-permeable channels and stomatal closure (2006) PLoS Biol, 4, pp. e327
  • Muñiz, G.M.N., País, S.M., Téllez-Iñón, M.T., Capiati, D.A., Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress (2011) Planta, 233, pp. 661-674
  • Murray, M.G., Thompson, W.F., Rapid isolation of high molecular weight plant DNA (1980) Nucleic Acids Res, 8, pp. 4321-4325
  • Orellana, S., Yañez, M., Espinoza, A., Verdugo, I., González, E., Ruiz-Lara, S., Casaretto, J.A., The transcription factor SlAREB1 confers drought, salt stress tolerance and regulates biotic and abiotic stress-related genes in tomato (2010) Plant Cell Environ, 33, pp. 2191-2208
  • País, S.M., González, M.A., Téllez-Iñón, M.T., Capiati, D.A., Characterization of potato (Solanum tuberosum) and tomato (Solanum lycopersicum) protein phosphatases type 2A catalytic subunits and their involvement in stress responses (2009) Planta, 230, pp. 13-25
  • País, S.M., Muñíz, G.M.N., Téllez-Iñón, M.T., Capiati, D.A., Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves (2010) Planta, 232, pp. 37-49
  • Poovaiah, B.W., Reddy, A.S.N., Calcium messenger system in plants (1987) CRC Crit Rev Plant Sci, 6, pp. 47-103
  • Raices, M., Gargantini, P.R., Chinchilla, D., Crespi, M., Tellez-Inon, M.T., Ulloa, R.M., Regulation of CDPK isoforms during tuber development (2003) Plant Mol Biol, 52, pp. 1011-1024
  • Reddy, V.S., Reddy, A.S., Proteomics of calcium-signaling components in plants (2004) Phytochemistry, 65, pp. 1745-1776
  • Reddy, A.S.N., Ali, G.S., Celesnik, H., Day, I.S., Coping with stresses: roles of calcium- and calcium/calmodulin-regulated gene expression (2011) Plant Cell, 23, pp. 2010-2032
  • Sarkar, D., The signal transduction pathways controlling in planta tuberization in potato: an emerging synthesis (2008) Plant Cell Rep, 27, pp. 1-8
  • Schütze, K., Harter, K., Chaban, C., Post-translational regulation of plant bZIP factors (2008) Trends Plant Sci, 13, pp. 247-255
  • Sheen, J., Ca 2+-dependent protein kinases and stress signal transduction in plants (1996) Science, 274, pp. 1900-1902
  • Genome sequence and analysis of the tuber crop potato (2011) Nature, 475, pp. 189-195. , The Potato Genome Sequencing Consortium
  • Uno, Y., Furihata, T., Abe, H., Yoshida, R., Shinozaki, K., Yamaguchi-Shinozaki, K., Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions (2000) Proc Natl Acad Sci USA, 97, pp. 11632-11637
  • Verslues, P.E., Zhu, J.K., Before and beyond ABA: upstream sensing and internal signals that determine ABA accumulation and response under abiotic stress (2005) Biochem Soc Trans, 33, pp. 375-379
  • Vreugdenhil, D., Struik, P.C., An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum) (1989) Physiol Plant, 75, pp. 525-531
  • Vreugdenhil, D., Xu, X., Jung, C.S., van Lammeren, A.A.M., Ewing, E.E., Initial anatomical changes associated with tuber formation on single-node potato (Solanum tuberosum L.) cuttings: a re-evaluation (1999) Ann Bot, 84, pp. 675-680
  • Wasilewska, A., Vlad, F., Sirichandra, C., Redko, Y., Jammes, F., Valon, C., Frei dit Frey, N., Leung, J., An update on abscisic acid signaling in plants and more (2008) Mol Plant, 1, pp. 198-217
  • Wenzler, H.C., Mignery, G.A., Fisher, L.M., Park, W.D., Analysis of a chimeric class-I Patatin-GUS gene in transgenic potato plants: high-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants (1989) Plant Mol Biol, 12, pp. 41-50
  • Xu, X., van Lammeren, A.A.M., Vermeer, E., Vreugdenhil, D., The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro (1998) Plant Physiol, 117, pp. 575-584
  • Yamagishi, K., Mitsumori, C., Takahashi, K., Fujino, K., Koda, Y., Kikuta, Y., Jasmonic acid-inducible gene expression of a Kunitz-type proteinase inhibitor in potato tuber disks (1993) Plant Mol Biol, 21, pp. 539-541
  • Yáñez, M., Cáceres, S., Orellana, S., Bastías, A., Verdugo, I., Ruiz-Lara, S., Casaretto, J.A., An abiotic stress-responsive bZIP transcription factor from wild and cultivated tomatoes regulates stress-related genes (2009) Plant Cell Rep, 28, pp. 1497-1507
  • Yoon, G.M., Cho, H.S., Ha, H.J., Liu, J.R., Lee, H.S., Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein (1999) Plant Mol Biol, 39, pp. 991-1001
  • Yu, X.C., Li, M.J., Gao, G.F., Feng, H.Z., Geng, X.Q., Peng, C.C., Zhu, S.Y., Zhang, D.P., Abscisic acid stimulates a calcium-dependent protein kinase in grape berry (2006) Plant Physiol, 140, pp. 558-579
  • Zhao, R., Sun, H.L., Mei, C., Wang, X.J., Yan, L., Liu, R., Zhang, X.F., Zhang, D.P., The Arabidopsis Ca 2+-dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth (2011) New Phytol, 192, pp. 61-73
  • Zhu, S.Y., Yu, X.C., Wang, X.J., Zhao, R., Li, Y., Fan, R.C., Shang, Y., Zhang, D.P., Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis (2007) Plant Cell, 19, pp. 3019-3036
  • Zou, J.J., Wei, F.J., Wang, C., Wu, J.J., Ratnasekera, D., Liu, W.X., Wu, W.H., Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca 2+-mediated stomatal regulation in response to drought stress (2010) Plant Physiol, 154, pp. 1232-1243

Citas:

---------- APA ----------
García, M.N.M., Giammaria, V., Grandellis, C., Téllez-Iñón, M.T., Ulloa, R.M. & Capiati, D.A. (2012) . Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro. Planta, 235(4), 761-778.
http://dx.doi.org/10.1007/s00425-011-1540-7
---------- CHICAGO ----------
García, M.N.M., Giammaria, V., Grandellis, C., Téllez-Iñón, M.T., Ulloa, R.M., Capiati, D.A. "Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro" . Planta 235, no. 4 (2012) : 761-778.
http://dx.doi.org/10.1007/s00425-011-1540-7
---------- MLA ----------
García, M.N.M., Giammaria, V., Grandellis, C., Téllez-Iñón, M.T., Ulloa, R.M., Capiati, D.A. "Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro" . Planta, vol. 235, no. 4, 2012, pp. 761-778.
http://dx.doi.org/10.1007/s00425-011-1540-7
---------- VANCOUVER ----------
García, M.N.M., Giammaria, V., Grandellis, C., Téllez-Iñón, M.T., Ulloa, R.M., Capiati, D.A. Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro. Planta. 2012;235(4):761-778.
http://dx.doi.org/10.1007/s00425-011-1540-7