Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Plasma membrane proton pumps (PM H+-ATPases) are involved in several physiological processes, such as growth and development, and abiotic stress responses. The major regulators of the PM H+-ATPases are proteins of the 14-3-3 family, which stimulate its activity. In addition, a novel interaction partner of the AHA1 PM H+-ATPase, named PPI1 (proton pump interactor, isoform 1), was identified in Arabidopsis thaliana. This protein stimulates the activity of the proton pump in vitro. In this work, we report the characterization of an A. thaliana PPI1 homolog in potato (Solanum tuberosum L.) named StPPI1. The full-length coding sequence of StPPI1 was obtained. The open reading frame (ORF) encodes a protein of 629 amino acids showing 50% identity with A. thaliana PPI1 protein. The StPPI1 ORF is divided into seven exons split by six introns. Southern blot analysis suggests that StPPI1 belongs to a family of related genes. Recombinant StPPI1 stimulates H+-ATPase activity in vitro. Basal levels of StPPI1 transcripts are observed in all tissues, however, StPPI1 expression is higher in proliferative regions (shoot apex and flower buds), flowers and leaves than in shoots and roots. StPPI1 mRNA levels significantly increase during tuber development. StPPI1 is induced by salt stress and cold. Drought and mechanical wounding slightly increase StPPI1 transcript levels. In addition, the expression of SlPPI1, the tomato homolog of StPPI1, was determined under adverse environmental conditions in tomato plants. SlPPI1 mRNA levels are increased by drought and cold, but are unaffected by salt stress. Mechanical wounding slightly increases SlPPI1 expression. © 2010 Springer-Verlag.

Registro:

Documento: Artículo
Título:Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
Autor:García, M.N.M.; País, S.M.; Téllez-Iñón, M.T.; Capiati, D.A.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 2, Buenos Aires C1428ADN, Argentina
Palabras clave:Plasma membrane H+-ATPase; Proton pump interactor; Solanum tuberosum L.; StPPI1; Stress; Tuber development; proton pump; proton transporting adenosine triphosphatase; recombinant protein; vegetable protein; amino acid sequence; antibody specificity; article; cell membrane; chemistry; enzymology; gene expression profiling; gene expression regulation; genetics; growth, development and aging; metabolism; molecular genetics; nucleotide sequence; pH; physiological stress; plant gene; plant tuber; potato; sequence alignment; Southern blotting; tomato; upregulation; Amino Acid Sequence; Blotting, Southern; Cell Membrane; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant; Hydrogen-Ion Concentration; Lycopersicon esculentum; Molecular Sequence Data; Organ Specificity; Plant Proteins; Plant Tubers; Proton Pumps; Proton-Translocating ATPases; Recombinant Proteins; Sequence Alignment; Solanum tuberosum; Stress, Physiological; Up-Regulation; Arabidopsis; Arabidopsis thaliana; Lycopersicon esculentum; Solanum tuberosum; Tuber (truffle)
Año:2011
Volumen:233
Número:4
Página de inicio:661
Página de fin:674
DOI: http://dx.doi.org/10.1007/s00425-010-1329-0
Título revista:Planta
Título revista abreviado:Planta
ISSN:00320935
CODEN:PLANA
CAS:Plant Proteins; Proton Pumps; Proton-Translocating ATPases, 3.6.3.14; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v233_n4_p661_Garcia

Referencias:

  • Anzi, C., Pelucchi, P., Vazzola, V., Murgia, I., Gomarasca, S., Piccoli, M.B., Morandini, P., The proton pump interactor (Ppi) gene family of Arabidopsis thaliana: expression pattern of Ppi1 and characterisation of knockout mutants for Ppi1 and 2 (2008) Plant Biol (Stuttg), 10, pp. 237-249
  • Arango, M., Gévaudant, F., Oufattole, M., Boutry, M., The plasma membrane proton pump ATPase: the significance of gene subfamilies (2003) Planta, 216, pp. 355-365
  • Baruah, K.K., Alyoshina, N.V., Astakhova, N.B., Trunova, T.I., Characterization of plasma membrane of winter wheat (Triticum aestivum L.) seedlings and changes in ATP hydrolyzing activity under low temperature stress (1990) Biol Plant, 32, pp. 211-217
  • Baxter, I., Tchieu, J., Sussman, M.R., Boutry, M., Palmgren, M.G., Gribskov, M., Harper, J.F., Axelsen, K.B., Genomic comparison of P-type ATPase ion pumps in Arabidopsis and rice (2003) Plant Physiol, 132, pp. 618-628
  • Bonza, M.C., Fusca, T., Homann, U., Thiel, G., de Michelis, M.I., Intracellular localisation of PPI1 (proton pump interactor, isoform 1), a regulatory protein of the plasma membrane H+-ATPase of Arabidopsis thaliana (2009) Plant Biol (Stuttg), 11, pp. 869-877
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Büchter, R., Strömberg, A., Schmelzer, E., Kombrink, E., Primary structure and expression of acidic (class II) chitinase in potato (1997) Plant Mol Biol, 35, pp. 749-761
  • Camoni, L., Iori, V., Marra, M., Aducci, P., Phosphorylation-dependent interaction between plant plasma membrane H+-ATPase and 14-3-3 proteins (2000) J Biol Chem, 275, pp. 9919-9923
  • Capiati, D.A., País, S.M., Téllez-Iñón, M.T., Wounding increases salt tolerance in tomato plants: evidence on the participation of calmodulin-like activities in cross-tolerance signalling (2006) J Exp Bot, 57, pp. 2391-2400
  • Chelysheva, V.V., Smolenskaya, I.N., Trofimova, M.C., Babakov, A.V., Muromtsev, G.S., Role of the 14-3-3 proteins in the regulation of H+-ATPase activity in the plasma membrane of suspension-cultured sugar beet cells under cold stress (1999) FEBS Lett, 456, pp. 22-26
  • Chen, Z., Pottosin, I.I., Cuin, T.A., Fuglsang, A.T., Tester, M., Jha, D., Zepeda-Jazo, I., Shabala, S., Root plasma membrane transporters controlling K+/Na+ homeostasis in salt-stressed barley (2007) Plant Physiol, 145, pp. 1714-1725
  • Duby, G., Poreba, W., Piotrowiak, D., Bobik, K., Derua, R., Waelkens, E., Boutry, M., Activation of plant plasma membrane H+-ATPase by 14-3-3 proteins is negatively controlled by two phosphorylation sites within the H+-ATPase C-terminal region (2009) J Biol Chem, 284, pp. 4213-4221
  • Ferl, R.J., 14-3-3 proteins: regulation of signal-induced events (2004) Physiol Plant, 120, pp. 173-178
  • Fuglsang, A.T., Visconti, S., Drumm, K., Jahn, T., Stensballe, A., Mattei, B., Jensen, O.N., Palmgren, M.G., Binding of 14-3-3 protein to the plasma membrane H+-ATPase AHA2 involves the three C-terminal residues Tyr(946)-Thr-Val and requires phosphorylation of Thr(947) (1999) J Biol Chem, 274, pp. 36774-36780
  • Gaxiola, R.A., Palmgren, M.G., Schumacher, K., Plant proton pumps (2007) FEBS Lett, 581, pp. 2204-2214
  • Gévaudant, F., Duby, G., von Stedingk, E., Zhao, R., Morsomme, P., Boutry, M., Expression of a constitutively activated plasma membrane H+-ATPase alters plant development and increases salt tolerance (2007) Plant Physiol, 144, pp. 1763-1776
  • Godoy, J.A., Pardo, J.M., Pintor-Toro, J.A., A tomato cDNA inducible by salt stress and abscisic acid: nucleotide sequence and expression pattern (1990) Plant Mol Biol, 26, pp. 1921-1934
  • Harms, K., Wohner, R.V., Schulz, B., Frommer, W.B., Isolation and characterization of P-type H+-ATPase genes from potato (1994) Plant Mol Biol, 26, pp. 979-988
  • Hebsgaard, S.M., Korning, P.G., Tolstrup, N., Engelbrecht, J., Rouze, P., Brunak, S., Splice site prediction in Arabidopsis thaliana DNA by combining local and global sequence information (1996) Nucleic Acids Res, 24, pp. 3439-3452
  • Hellergren, J., Widell, S., Lundborg, T., Kylin, A., Frost hardiness development in Pinus sylvestris: the involvement of a K+-stimulated Mg2+-dependent ATPase from purified plasma membranes of pine (1983) Physiol Plant, 58, pp. 7-12
  • Hiribayashi, S., Matsushita, Y., Sato, M., Ohi, R., Kasahara, M., Abe, H., Nyunoya, H., Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase (2004) Plant Biotechnol, 21, pp. 35-45
  • Hohenwallner, W., Wimmer, E., Malachite green micromethod for determination of inorganic-phosphate (1973) Clin Chim Acta, 45, pp. 169-175
  • Jahn, T., Fuglsang, A.T., Olsson, A., Bruntrup, I.M., Collinge, D.B., Volkmann, D., Sommarin, M., Larsson, C., The 14-3-3 protein interacts directly with the C-terminal region of the plant plasma membrane H+-ATPase (1997) Plant Cell, 9, pp. 1805-1814
  • Janicka-Russak, M., Klobus, G., Modification of plasma membrane and vacuolar H+-ATPases in response to NaCl and ABA (2007) J Plant Physiol, 164, pp. 295-302
  • Johansson, F., Olbe, M., Sommarin, M., Larsson, C., Brij 58, a polyoxyethylene acyl ether, creates membrane vesicles of uniform sidedness. A new tool to obtain inside-out (cytoplasmic side-out) plasma membrane vesicles (1995) Plant J, 7, pp. 165-173
  • Kalampanayil, B.D., Wimmers, L.E., Identification and characterization of a salt-stress-induced plasma membrane H+-ATPase in tomato (2001) Plant Cell Environ, 24, pp. 999-1000
  • López-Pérez, L., Martínez-Ballesta, M.C., Maurel, C., Carvajal, M., Changes in plasma membrane lipids, aquaporins and proton pump of broccoli roots, as an adaptation mechanism to salinity (2009) Phytochemistry, 70, pp. 492-500
  • Martz, F., Sutinen, M.L., Kiviniemi, S., Palta, J.P., Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation (2006) Tree Physiol, 26, pp. 783-790
  • Mattheis, J.P., Ketchie, D.O., Changes in parameters of the plasmalemma ATPase during cold acclimation of apple (Malus domestica) tree bark tissues (1990) Physiol Plant, 78, pp. 616-622
  • Morandini, P., Valera, M., Albumi, C., Bonza, M.C., Giacometti, S., Murgia, I., Soave, C., de Michelis, M.I., A novel interaction partner for the C-terminus of Arabidopsis thaliana plasma membrane H+-ATPase (AHA1 isoform): site and mechanism of action on H+-ATPase activity differ from those of 14-3-3 proteins (2002) Plant J, 31, pp. 487-497
  • Murray, M.G., Thompson, W.F., Rapid isolation of high molecular weight plant DNA (1980) Nucleic Acids Res, 8, pp. 4321-4325
  • Olivari, C., Albumi, C., Pugliarello, M.C., de Michelis, M.I., Phenylarsine oxide inhibits the fusicoccin-induced activation of plasma membrane H+-ATPase (2000) Plant Physiol, 122, pp. 463-470
  • Ottmann, C., Marco, S., Jaspert, N., Marcon, C., Schauer, N., Weyand, M., Vandermeeren, C., Oecking, C., Structure of a 14-3-3 coordinated hexamer of the plant plasma membrane H+-ATPase by combining X-ray crystallography and electron cryomicroscopy (2007) Mol Cell, 25, pp. 427-440
  • País, S.M., González, M.A., Téllez-Iñón, M.T., Capiati, D.A., Characterization of potato (Solanum tuberosum) and tomato (Solanum lycopersicum) protein phosphatases type 2A catalytic subunits and their involvement in stress responses (2009) Planta, 230, pp. 13-25
  • País, S.M., Muñíz García, M.N., Téllez-Iñón, M.T., Capiati, D.A., Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves (2010) Planta, 232, pp. 37-49
  • Palmgren, M.G., Plant plasma membrane H+-ATPases: powerhouses for nutrient uptake (2001) Annu Rev Plant Physiol Plant Mol Biol, 52, pp. 817-845
  • Papini, R., de Michelis, M.I., Changes in the level and activation state of the plasma membrane H+-ATPase during aging of red beet slices (1997) Plant Physiol, 114, pp. 857-862
  • Rensink, W.A., Lobst, S., Hart, A., Stegalkina, S., Liu, J., Buell, C.R., Gene expression profiling of potato responses to cold, heat, and salt stress (2005) Funct Integr Genomics, 5, pp. 201-207
  • Rober-Kleber, N., Albrechtová, J.T., Fleig, S., Huck, N., Michalke, W., Wagner, E., Speth, V., Fischer-Iglesias, C., Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development (2003) Plant Physiol, 13, pp. 1302-1312
  • Sahu, B.B., Shaw, B.P., Salt-inducible isoform of plasma membrane H+-ATPase gene in rice remains constitutively expressed in natural halophyte, Suaeda maritima (2009) J Plant Physiol, 166, pp. 1077-1089
  • Sanchez-Serrano, J., Scmidt, R., Schell, J., Willmitzer, L., Nucleotide sequence of proteinase inhibitor II encoding cDNA of potato (Solanum tuberosum) and its mode of expression (1986) Mol Gen Genet, 203, pp. 15-20
  • Schaller, G.E., Dewitt, N.D., Analysis of H+-ATPase and other proteins of the Arabidopsis plasma membrane (1995) Methods Cell Biol, 50, pp. 129-148
  • Shi, Y., An, L., Zhang, M., Huang, C., Zhang, H., Xu, S., Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana) (2008) Protoplasma, 232, pp. 173-781
  • Sibole, J.V., Cabot, C., Michalke, W., Poschenrieder, C., Barceló, J., Relationship between expression of the PM H+-ATPase, growth and ion partitioning in the leaves of salt-treated Medicago species (2005) Planta, 221, pp. 557-566
  • Sondergaard, T.E., Schulz, A., Palmgren, M.G., Energization of transport processes in plants. Roles of the plasma membrane H+-ATPase (2004) Plant Physiol, 136, pp. 2475-2482
  • Sveinsdóttir, H., Yan, F., Zhu, Y., Peiter-Volk, T., Schubert, S., Seed ageing-induced inhibition of germination and post-germination root growth is related to lower activity of plasma membrane H+-ATPase in maize roots (2009) J Plant Physiol, 166, pp. 128-135
  • Viotti, C., Luoni, L., Morandini, P., de Michelis, M.I., Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) (2005) FEBS J, 272, pp. 5864-5871
  • Vitart, V., Baxter, I., Doerner, P., Harper, J.F., Evidence for a role in growth and salt resistance of a plasma membrane H+-ATPase in the root endodermis (2001) Plant J, 27, pp. 191-201
  • Wu, J., Seliskar, D., Salinity adaptation of plasma membrane H+-ATPase in the salt marsh plant Spartina patens: ATP hydrolysis and enzyme kinetics (1998) J Exp Bot, 49, pp. 1005-1013
  • Yamagishi, K., Mitsumori, C., Takahashi, K., Fujino, K., Koda, Y., Kikuta, Y., Jasmonic acid-inducible gene expression of a Kunitz-type proteinase inhibitor in potato tuber disks (1993) Plant Mol Biol, 21, pp. 539-541
  • Zhao, R., Dielen, V., Kinet, J.M., Boutry, M., Cosuppression of a plasma membrane H+-ATPase isoform impairs sucrose translocation, stomatal opening, plant growth, and male fertility (2000) Plant Cell, 12, pp. 535-546

Citas:

---------- APA ----------
García, M.N.M., País, S.M., Téllez-Iñón, M.T. & Capiati, D.A. (2011) . Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress. Planta, 233(4), 661-674.
http://dx.doi.org/10.1007/s00425-010-1329-0
---------- CHICAGO ----------
García, M.N.M., País, S.M., Téllez-Iñón, M.T., Capiati, D.A. "Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress" . Planta 233, no. 4 (2011) : 661-674.
http://dx.doi.org/10.1007/s00425-010-1329-0
---------- MLA ----------
García, M.N.M., País, S.M., Téllez-Iñón, M.T., Capiati, D.A. "Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress" . Planta, vol. 233, no. 4, 2011, pp. 661-674.
http://dx.doi.org/10.1007/s00425-010-1329-0
---------- VANCOUVER ----------
García, M.N.M., País, S.M., Téllez-Iñón, M.T., Capiati, D.A. Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress. Planta. 2011;233(4):661-674.
http://dx.doi.org/10.1007/s00425-010-1329-0