La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the roles of protein phosphatase type 2A catalytic subunits (PP2Ac) in the leaf responses to conditions that affect tuberization. The responses were monitored by analyzing the expression of the "tuber-specific" genes Patatin and Pin2, which are induced in tubers and leaves during tuber induction. Experiments using PP2A inhibitors, together with PP2Ac expression profiles under conditions that affect tuberization indicate that high sucrose/nitrogen ratio, which promotes tuber formation, increases the transcript levels of Patatin and Pin2, by increasing the activity of PP2As without affecting PP2Ac mRNA or protein levels. Gibberellic acid (GA), a negative regulator of tuberization, down-regulates the transcription of catalytic subunits of PP2As from the subfamily I and decreases their enzyme levels. In addition, GA inhibits the expression of Patatin and Pin2 possibly by a PP2A-independent mechanism. PP2Ac down-regulation by GA may inhibit tuberization signaling downstream of the inductive effects of high sucrose/nitrogen ratio. These results are consistent with the hypothesis that PP2As of the subfamily I may positively modulate the signaling pathways that lead to the transcriptional activation of "tuber-specific" genes in leaves, and act as molecular switches regulated by both positive and negative modulators of tuberization. © 2010 Springer-Verlag.


Documento: Artículo
Título:Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
Autor:País, S.M.; García, M.N.M.; Téllez-Iñón, M.T.; Capiati, D.A.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 20 piso, C1428ADN Buenos Aires, Argentina
Palabras clave:Gibberellic acid; Patatin; Pin2; PP2A; Sucrose; Tuberization; phosphoprotein phosphatase 2; primer DNA; amino acid sequence; article; chemistry; enzyme active site; enzymology; metabolism; molecular genetics; nucleotide sequence; plant leaf; potato; reverse transcription polymerase chain reaction; sequence homology; signal transduction; Western blotting; Amino Acid Sequence; Base Sequence; Blotting, Western; Catalytic Domain; DNA Primers; Molecular Sequence Data; Plant Leaves; Protein Phosphatase 2; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Signal Transduction; Solanum tuberosum; Solanum tuberosum
Página de inicio:37
Página de fin:49
Título revista:Planta
Título revista abreviado:Planta
CAS:DNA Primers; Protein Phosphatase 2,


  • Banerjee, A.K., Chatterjee, M., Yu, Y., Suh, S.G., Miller, W.A., Hannapel, D.J., Dynamics of a mobile RNA of potato involved in a long-distance signaling pathway (2006) Plant Cell, 18, pp. 3443-3457
  • Banerjee, A.K., Lin, T., Hannapel, D.J., Untranslated regions of a mobile transcript mediate RNA metabolism (2009) Plant Physiol, 151, pp. 1831-1843
  • Carrera, E., Jackson, S.D., Prat, S., Feedback control and diurnal regulation of gibberellin 20-oxidase transcript levels in potato (1999) Plant Physiol, 119, pp. 765-774
  • Carrera, E., Bou, J., García-Martínez, J.L., Prat, S., Changes in GA 20-oxidase gene expression strongly affect stem length, tuber induction and tuber yield of potato plants (2000) Plant J, 22, pp. 247-256
  • Chincinska, I.A., Liesche, J., Krügel, U., Michalska, J., Geigenberger, P., Grimm, B., Kühn, C., Sucrose transporter StSUT4 from potato affects flowering, tuberization, and shade avoidance response (2008) Plant Physiol, 146, pp. 515-528
  • Farkas, I., Dombrádi, V., Miskei, M., Szabados, L., Koncz, C., Arabidopsis PPP family of serine/threonine phosphatases (2007) Trends Plant Sci, 12, pp. 169-176
  • Fischer, L., Lipavska, H., Hausman, J.F., Opatrny, Z., Morphological and molecular characterization of a spontaneously tuberizing potato mutant: An insight into the regulatory mechanisms of tuber induction (2008) BMC Plant Biol, 8, p. 117
  • Fusari, C., Demonte, A.M., Figueroa, C.M., Aleanzi, M., Iglesias, A.A., A colorimetric method for the assay of ADP-glucose pyrophosphorylase (2006) Anal Biochem, 352, pp. 145-147
  • Hannapel, D.J., Differential expression of potato tuber protein genes (1990) Plant Physiol, 94, pp. 919-925
  • Hannapel, D.J., Miller, J.C., Park, W.D., Regulation of potato tuber protein accumulation by gibberellic acid (1985) Plant Physiol, 78, pp. 700-703
  • Hohenwallner, W., Wimmer, E., Malachite green micromethod for determination of inorganic-phosphate (1973) Clin Chim Acta, 45, pp. 169-175
  • Jackson, S.D., Multiple signaling pathways control tuber induction in potato (1999) Plant Physiol, 119, pp. 1-8
  • Jackson, S.D., Plant responses to photoperiod (2009) New Phytol, 181, pp. 517-531
  • Jackson, S.D., James, P., Prat, S., Thomas, B., Phytochrome B affects the levels of a graft-transmissible signal involved in tuberization (1998) Plant Physiol, 117, pp. 29-32
  • Janssens, V., Goris, J., Protein phosphatase 2A: A highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling (2001) Biochem J, 353, pp. 417-439
  • Kloosterman, B., Navarro, C., Bijsterbosch, G., Lange, T., Prat, S., Visser, R.G., Bachem, C.W., StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development (2007) Plant J, 52, pp. 362-373
  • Larsen, P.B., Cancel, J.D., Enhanced ethylene responsiveness in the Arabidopsis eer1 mutant results from a loss-of-function mutation in the protein phosphatase 2A A regulatory subunit, RCN1 (2003) Plant J, 34, pp. 709-718
  • Lorenzen, J.H., Ewing, E.E., Starch accumulation in leaves of potato (Solanum tuberosum L.) during the 1st 18 days of photoperiod treatment (1992) Ann Bot, 69, pp. 481-485
  • Luan, S., Protein phosphatases in plants (2003) Annu Rev Plant Biol, 54, pp. 63-92
  • Machackova, I., Konstantinova, T.N., Sergeeva, L.I., Lozhnikova, V.N., Golyanovskaya, S.A., Dudko, N.D., Eder, J., Aksenova, N.P., Photoperiodic control of growth, development and phytohormone balance in Solanum tuberosum (1998) Physiol Plant, 102, pp. 272-278
  • Martin, A., Adam, H., Díaz-Mendoza, M., Zurczak, M., González-Schain, N.D., Suárez-López, P., Graft-transmissible induction of potato tuberization by the microRNA miR172 (2009) Development, 136, pp. 2873-2881
  • Martínez-García, J.F., García-Martínez, J.L., Bou, J., Prat, S., The interaction of gibberellins and photoperiod in the control of potato tuberization (2002) J Plant Growth Regul, 20, pp. 377-386
  • País, S.M., González, M.A., Téllez-Iñón, M.T., Capiati, D.A., Characterization of potato (Solanum tuberosum) and tomato (Solanum lycopersicum) protein phosphatases type 2A catalytic subunits and their involvement in stress responses (2009) Planta, 230, pp. 13-25
  • Prickett, T.D., Brautigan, D.L., The alpha4 regulatory subunit exerts opposing allosteric effects on protein phosphatases PP6 and PP2A (2006) J Biol Chem, 281, pp. 30503-30511
  • Raíces, M., Chico, J.M., Téllez-Iñón, M.T., Ulloa, R.M., Molecular characterization of StCDPK1, a calcium-dependent protein kinase from Solanum tuberosum that is induced at the onset of tuber development (2001) Plant Mol Biol, 46, pp. 591-601
  • Raíces, M., Macintosh, G.C., Ulloa, R.M., Gargantini, P.R., Vozza, N.F., Téllez-Inón, M.T., Sucrose increases calcium-dependent protein kinase and phosphatase activities in potato plants (2003) Cell Mol Biol, 49, pp. 959-964
  • Raíces, M., Ulloa, R.M., Macintosh, G.C., Crespi, M., Téllez-Iñón, M.T., StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration (2003) J Exp Bot, 54, pp. 2589-2591
  • Rocha-Sosa, M., Sonnewald, U., Frommer, W., Stratmann, M., Schell, J., Willmitzer, L., Both developmental and metabolic signals activate the promoter of a class I Patatin gene (1989) EMBO J, 8, pp. 23-29
  • Rodríguez-Falcón, M., Bou, J., Prat, S., Seasonal control of tuberization in potato: Conserved elements with the flowering response (2006) Annu Rev Plant Biol, 57, pp. 151-180
  • Sarkar, D., The signal transduction pathways controlling in planta tuberization in potato: An emerging synthesis (2008) Plant Cell Rep, 27, pp. 1-8
  • Schönthal, A.H., Role of PP2A in intracellular signal transduction pathways (1998) Front Biosci, 3, pp. D1262-D1273
  • Shi, Y., Serine/threonine phosphatases: Mechanism through structure (2009) Cell, 139, pp. 468-484
  • Suárez-López, P., Long-range signalling in plant reproductive development (2005) Int J Dev Biol, 49, pp. 761-771
  • Vreugdenhil, D., Xu, X., Jung, C.S., van Lammeren, A.A.M., Ewing, E.E., Initial anatomical changes associated with tuber formation on single-node potato (Solanum tuberosum L.) cuttings: A re-evaluation (1999) Ann Bot, 84, pp. 675-680
  • Wei, H., Ashby, D.G., Moreno, C.S., Ogris, E., Yeong, F.M., Corbett, A.H., Pallas, D.C., Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p (2001) J Biol Chem, 276, pp. 1570-1577
  • Wenzler, H.C., Mignery, G.A., Fisher, L.M., Park, W.D., Analysis of a chimeric class-I Patatin-GUS gene in transgenic potato plants: High-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants (1989) Plant Mol Biol, 12, pp. 41-50
  • Xu, X., van Lammeren, A.A.M., Vermeer, E., Vreugdenhil, D., The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro (1998) Plant Physiol, 117, pp. 575-584
  • Yamagishi, K., Mitsumori, C., Takahashi, K., Fujino, K., Koda, Y., Kikuta, Y., Jasmonic acid-inducible gene expression of a Kunitz-type proteinase inhibitor in potato tuber disks (1993) Plant Mol Biol, 21, pp. 539-541
  • Yu, X.X., Du, X., Moreno, C.S., Green, R.E., Ogris, E., Feng, Q., Chou, L., Pallas, D.C., Methylation of the protein phosphatase 2A catalytic subunit is essential for association of B-alpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen (2001) Mol Biol Cell, 12, pp. 185-199
  • Zolnierowicz, S., Type 2A protein phosphatase, the complex regulator of numerous signaling pathways (2000) Biochem Pharmacol, 60, pp. 1225-1235


---------- APA ----------
País, S.M., García, M.N.M., Téllez-Iñón, M.T. & Capiati, D.A. (2010) . Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves. Planta, 232(1), 37-49.
---------- CHICAGO ----------
País, S.M., García, M.N.M., Téllez-Iñón, M.T., Capiati, D.A. "Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves" . Planta 232, no. 1 (2010) : 37-49.
---------- MLA ----------
País, S.M., García, M.N.M., Téllez-Iñón, M.T., Capiati, D.A. "Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves" . Planta, vol. 232, no. 1, 2010, pp. 37-49.
---------- VANCOUVER ----------
País, S.M., García, M.N.M., Téllez-Iñón, M.T., Capiati, D.A. Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves. Planta. 2010;232(1):37-49.