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Abstract:

In organello starch biosynthesis was studied using intact chloroplasts isolated from spinach leaves (Spinacia oleracea). Immunoblot analysis using a specific antiserum against the mitochondrial adenylate (ADP/ATP) translocator of Neurospora crassa shows the presence of an adenylate translocator protein in the chloroplast envelope membranes, similar to that existing in mitochondria and amyloplasts from cultured cells of sycamore (Acer pseudoplatanus). The double silicone oil layer-filtering centrifugation technique was employed to study the kinetic properties of adenylate transport in the purified chloroplasts; ATP, ADP, AMP, and most importantly ADP-Glc were shown to be recognized by the adenylate translocator. Similar to the situation with sycamore amyloplasts, only ATP and ADP-Glc uptake was inhibited by carboxyatractyloside, an inhibitor of the mitochondrial adenylate translocator. Evidence is presented to show that the ADP-Glc transported into the chloroplast stroma is utilized for starch synthesis catalyzed by starch synthase (ADP-Glc: 1,4-α-d-glucan 4-α-d-glucosyltransferase). The high activity of sucrose synthase producing ADP-Glc observed in the extrachloroplastic fractions suggests that starch biosynthesis in chloroplasts may be coupled with the direct import of ADP-Glc from the cytosol.

Registro:

Documento: Artículo
Título:ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
Autor:Pozueta-Romero, J.; Ardila, F.; Akazawa, T.
Filiación:Res. Inst. for Biochem. Regulation, School of Agriculture, Nagoya University, Chikusa, Nagoya 464-01, Japan
Inst. Invest. Boquimicas F. C., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Acer; Acer pseudoplatanus; Neurospora crassa; Spinacia oleracea
Año:1991
Volumen:97
Número:4
Página de inicio:1565
Página de fin:1572
DOI: http://dx.doi.org/10.1104/pp.97.4.1565
Título revista:Plant Physiology
Título revista abreviado:Plant Physiol.
ISSN:00320889
CODEN:PLPHA
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00320889_v97_n4_p1565_PozuetaRomero.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v97_n4_p1565_PozuetaRomero

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Citas:

---------- APA ----------
Pozueta-Romero, J., Ardila, F. & Akazawa, T. (1991) . ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis. Plant Physiology, 97(4), 1565-1572.
http://dx.doi.org/10.1104/pp.97.4.1565
---------- CHICAGO ----------
Pozueta-Romero, J., Ardila, F., Akazawa, T. "ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis" . Plant Physiology 97, no. 4 (1991) : 1565-1572.
http://dx.doi.org/10.1104/pp.97.4.1565
---------- MLA ----------
Pozueta-Romero, J., Ardila, F., Akazawa, T. "ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis" . Plant Physiology, vol. 97, no. 4, 1991, pp. 1565-1572.
http://dx.doi.org/10.1104/pp.97.4.1565
---------- VANCOUVER ----------
Pozueta-Romero, J., Ardila, F., Akazawa, T. ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis. Plant Physiol. 1991;97(4):1565-1572.
http://dx.doi.org/10.1104/pp.97.4.1565