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To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics. © 2011 American Society of Plant Biologists.


Documento: Artículo
Título:Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
Autor:Sutka, M.; Li, G.; Boudet, J.; Boursiac, Y.; Doumas, P.; Maurel, C.
Filiación:Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Universite, F-34060 Montpellier cedex 2, France
Diversité et Adaptation des Plantes Cultivées, Equipe Rhizogénese, UMR 188 Institut de Recherche pour le Developpement/INRA/Montpellier SupAgro/ Universite, F-34394 Montpellier cedex 5, France
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160 Piso 4 (C1428EGA), Buenos Aires, Argentina
Diversité et Ecophysiologie des Céréales, UMR 1095 INRA/Université Blaise Pascal, Campus des Cézeaux, 24 avenue des Landais, BP 80026, F-63171 Aubière, France
Palabras clave:aquaporin; messenger RNA; sodium chloride; water; Arabidopsis; article; drug effect; gene expression profiling; gene expression regulation; genetic variability; genetics; growth, development and aging; histology; metabolism; physiological stress; physiology; plant root; principal component analysis; Aquaporins; Arabidopsis; Gene Expression Profiling; Gene Expression Regulation, Plant; Genetic Variation; Plant Roots; Principal Component Analysis; RNA, Messenger; Sodium Chloride; Stress, Physiological; Water; Arabidopsis; Arabidopsis thaliana
Página de inicio:1264
Página de fin:1276
Título revista:Plant Physiology
Título revista abreviado:Plant Physiol.
CAS:aquaporin, 215587-75-0; sodium chloride, 7647-14-5; water, 7732-18-5; Aquaporins; RNA, Messenger; Sodium Chloride, 7647-14-5; Water, 7732-18-5


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---------- APA ----------
Sutka, M., Li, G., Boudet, J., Boursiac, Y., Doumas, P. & Maurel, C. (2011) . Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions. Plant Physiology, 155(3), 1264-1276.
---------- CHICAGO ----------
Sutka, M., Li, G., Boudet, J., Boursiac, Y., Doumas, P., Maurel, C. "Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions" . Plant Physiology 155, no. 3 (2011) : 1264-1276.
---------- MLA ----------
Sutka, M., Li, G., Boudet, J., Boursiac, Y., Doumas, P., Maurel, C. "Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions" . Plant Physiology, vol. 155, no. 3, 2011, pp. 1264-1276.
---------- VANCOUVER ----------
Sutka, M., Li, G., Boudet, J., Boursiac, Y., Doumas, P., Maurel, C. Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions. Plant Physiol. 2011;155(3):1264-1276.