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Abstract:

In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals. © 2008 American Society of Plant Biologists.

Registro:

Documento: Artículo
Título:The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
Autor:Zhang, D.; Wengier, D.; Shuai, B.; Gui, C.-P.; Muschietti, J.; McCormick, S.; Tang, W.-H.
Filiación:Shanghai Institutes for Biol. Sci.-Univ. of California at Berkeley Center of Molecular Life Sciences, National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai 200032, China
Instituto de Ingeniería Genética Y Biología Molecular, CONICET Departamento de Fisiología Y Biología Molecular Y Celular FCEN, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
Plant Gene Expression Center, Department of Plant and Microbial Biology, University of California at Berkeley, Albany, CA 94710, United States
Department of Biological Sciences, Wichita State University, Wichita, KS 67260, United States
Palabras clave:hybrid protein; primer DNA; protein kinase; article; enzymology; germination; growth, development and aging; metabolism; molecular genetics; nucleotide sequence; physiology; pollen; polymerase chain reaction; signal transduction; tomato; transgenic plant; Base Sequence; DNA Primers; Germination; Lycopersicon esculentum; Molecular Sequence Data; Plants, Genetically Modified; Pollen; Polymerase Chain Reaction; Protein Kinases; Recombinant Fusion Proteins; Signal Transduction; Lycopersicon esculentum; Magnoliophyta; Solanum
Año:2008
Volumen:148
Número:3
Página de inicio:1368
Página de fin:1379
DOI: http://dx.doi.org/10.1104/pp.108.124420
Título revista:Plant Physiology
Título revista abreviado:Plant Physiol.
ISSN:00320889
CODEN:PLPHA
CAS:protein kinase, 9026-43-1; DNA Primers; Protein Kinases, EC 2.7.1.37; Recombinant Fusion Proteins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00320889_v148_n3_p1368_Zhang.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v148_n3_p1368_Zhang

Referencias:

  • Bassham, D.C., Raikhel, N.V., Unique features of the plant vacuolar sorting machinery (2000) Curr Opin Cell Biol, 12, pp. 491-495
  • Benkert, R., Obermeyer, G., Bentrup, F.W., The turgor pressure of growing lily pollen tubes (1997) Protoplasma, 198, pp. 1-8
  • Berken, A., Thomas, C., Wittinghofer, A., A new family of RhoGEFs activates the Rop molecular switch in plants (2005) Nature, 436, pp. 1176-1180
  • Campbell, R.E., Tour, O., Palmer, A.E., Steinbach, P.A., Baird, B.S., Zacharias, D.A., Tsien, R.Y., A monomeric red fluorescent protein (2002) Proc Natl Acad Sci USA, 99, pp. 7877-7882
  • Cárdenas, L., Lovy-Wheeler, A., Kunkel, J.G., Hepler, P.K., Pollen tube growth oscillations and intracellular calcium levels are reversibly modulated by actin polymerization (2008) Plant Physiol, 146, pp. 1611-1621
  • Cárdenas, L., McKenna, S.T., Kunkel, J.G., Hepler, P.K., NAD(P)H oscillates in pollen tubes and is correlated with tip growth (2006) Plant Physiol, 142, pp. 1460-1468
  • Foreman, J., Demidchik, V., Bothwell, J.H.F., Mylona, P., Miedema, H., Torres, M.A., Linstead, P., Jones, J.D.G., Reactive oxygen species produced by NADPH oxidase regulate plant cell growth (2003) Nature, 422, pp. 442-446
  • Guyon, V., Tang, W., Monti, M., Raiola, A., DeLorenzo, G., McCormick, S., Taylor, L., Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth (2004) Plant J, 39, pp. 643-654
  • Hicks, G., Rojo, E., Hong, S., Carter, D., Raikhel, N., Geminating pollen has tubular vacuoles, displays highly dynamic vacuole biogenesis, and requires VACUOLELESS1 for proper function (2004) Plant Physiol, 134, pp. 1227-1239
  • Hoekema, A., Hirsch, P.R., Hooykaas, P., Schilperoort, R.A., A binary plant vector strategy based on separation of vir and T-region of Agrobacterium tumefaciens Ti plasmid (1983) Nature, 303, pp. 179-180
  • Johnson-Brousseau, S., McCormick, S., A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically- expressed genes (2004) Plant J, 39, pp. 761-775
  • Johri, B.M., Vasil, I.K., Physiology of pollen (1961) Bot Rev, 27, pp. 325-381
  • Kaothien, P., Ok, S.H., Shuai, B., Wengier, D., Cotter, R., Kelley, D., Kiriakopolos, S., McCormick, S., Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth (2005) Plant J, 42, pp. 492-503
  • Keller, T., Damude, H., Werner, D., Doerner, P., Dixon, R., Lamb, C., A plant homolog of the neutrophil NADPH oxidase gp91phox subunit gene encodes a plasma membrane protein with Ca2+ binding motifs (1998) Plant Cell, 10, pp. 255-266
  • Kim, H.Y., Cotter, R., Johnson, S., Senda, M., Dodds, P., Kulikauskas, R., Tang, W., McCormick, S., New pollen-specific receptor kinases identified in tomato, maize and Arabidopsis: The tomato kinases show overlapping but distinct localization patterns on pollen tubes (2002) Plant Mol Biol, 50, pp. 1-16
  • Krichevsky, A., Kozlovsky, S.V., Tian, G.W., Chen, M.H., Zaltsman, A., Citovsky, V., How pollen tubes grow (2007) Dev Biol, 303, pp. 405-420
  • Lee, Y.J., Szumlanski, A., Yang, Z., Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth (2008) J Cell Biol, 181, pp. 1155-1168
  • Lew, R., Osmotic effects on the electrical properties of Arabidopsis root hair vacuoles in situ (2004) Plant Physiol, 134, pp. 352-360
  • Li, H., Lin, Y., Heath, R.M., Zhu, M.X., Yang, Z., Control of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to tip-localized calcium influx (1999) Plant Cell, 11, pp. 1731-1742
  • Lovy-Wheeler, A., Cárdenas, L., Kunkel, J.G., Hepler, P.K., Differential organelle movement on the actin cytoskeleton in lily pollen tubes (2007) Cell Motil Cytoskeleton, 64, pp. 217-232
  • MacRobbie, E., Osmotic effects on vacuolar ion release in guard cells (2006) Proc Natl Acad Sci USA, 103, pp. 1135-1140
  • McCormick, S., Transformation of tomato with Agrobacterium tumefaciens (1991) Plant Tissue Culture Manual, Fundamentals and Applications, B, pp. 1-9. , K Lindsey, ed, Kluwer, Amsterdam, pp
  • Mittler, R., Oxidative stress, antioxidants and stress tolerance (2002) Trends Plant Sci, 7, pp. 405-410
  • Mori, I.C., Schroeder, J.I., Reactive oxygen species activation of plant Ca2+ channels: A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetically mechanotransduction (2004) Plant Physiol, 135, pp. 702-708
  • Muschietti, J.P., Dircks, L., Vancanneyt, G., McCormick, S., LAT52 protein is essential for tomato pollen development: Pollen expressing antisense LAT52 RNA hydrates and germinates abnormally and cannot achieve fertilization (1994) Plant J, 6, pp. 321-338
  • Muschietti, J.P., Eyal, Y., McCormick, S., Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 (1998) Plant Cell, 10, pp. 319-330
  • Nishikawa, S., Zinkl, G.M., Swanson, R.J., Maruyama, D., Preuss, D., Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth (2005) BMC Plant Biol, 5, p. 22
  • Potocký, M., Jones, M.A., Bezvoda, R., Smirnoff, N., Zárský, V., Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth (2007) New Phytol, 174, pp. 742-751
  • Rossetti, S., Bonatti, P.M., In situ histochemical monitoring of ozone- and TMV-induced reactive oxygen species in tobacco leaves (2001) Plant Physiol Biochem, 39, pp. 433-442
  • Steer, M., Steer, J., Pollen tube tip growth (1989) New Phytol, 111, pp. 323-358
  • Takeda, S., Gapper, C., Kaya, H., Bell, E., Kuchitsu, K., Dolan, L., Local positive feedback regulation determines cell shape in root hair cells (2008) Science, 319, pp. 1241-1244
  • Tang, W., Coughlan, S., Crane, E., Beatty, M., Duvick, J., The application of laser microdissection to in planta gene expression profiling of the maize anthracnose stalk rot fungus Colletotrichum graminicola (2006) Mol Plant Microbe Interact, 19, pp. 1240-1250
  • Tang, W., Ezcurra, I., Muschietti, J., McCormick, S., A cysteine-rich extracellular protein, LAT52, interacts with the extracellular domain of the pollen receptor kinase LePRK2 (2002) Plant Cell, 14, pp. 2277-2287
  • Tang, W., Kelley, D., Ezcurra, I., Cotter, R., McCormick, S., LeSTIG1, an extracellular binding partner for the pollen receptor kinases LePRK1 and LePRK2, promotes pollen tube growth in vitro (2004) Plant J, 39, pp. 343-353
  • Taylor, L.P., Hepler, P., Pollen germination and tube growth (1997) Annu Rev Plant Physiol Plant Mol Biol, 48, pp. 461-491
  • Taylor, W.R., Jones, D.T., Segal, A.W., A structural model for the nucleotide binding domains of the flavocytochrome b-245 β-chain (1993) Protein Sci, 2, pp. 1675-1685
  • Toews, M.W., Warmbold, J., Konzack, S., Rischitor, P., Veith, D., Vienken, K., Vinuesa, C., Fischer, R., Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (GATEWAY) (2004) Curr Genet, 45, pp. 383-389
  • Twell, D., Klein, T.M., Fromm, M.E., McCormick, S., Transient expression of chimeric genes delivered into pollen by microprojectile bombardment (1989) Plant Physiol, 91, pp. 1270-1274
  • Twell, D., Wing, R., Yamaguchi, J., McCormick, S., Isolation and expression of an anther-specific gene from tomato (1989) Mol Gen Genet, 217, pp. 240-245
  • Twell, D., Yamaguchi, J., Wing, R.A., Ushiba, J., McCormick, S., Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements (1991) Genes Dev, 5, pp. 496-507
  • Wengier, D., Valsecchi, I., Cabanas, M.L., Tang, W., McCormick, S., Muschietti, J., The receptor kinases LePRK1 and LePRK2 associate in pollen and when expressed in yeast, but dissociate in the presence of style extract (2003) Proc Natl Acad Sci USA, 100, pp. 6860-6865
  • Williams, L., Fletcher, J.C., Stem cell regulation in the Arabidopsis shoot apical meristem (2005) Curr Opin Plant Biol, 8, pp. 582-586
  • Wong, H.L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Kawasaki, T., Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension (2007) Plant Cell, 19, pp. 4022-4034
  • Zhang, Y., McCormick, S., A distinct mechanism regulating a pollen-specific guanine nucleotide exchange factor for the small GTPase Rop in Arabidopsis thaliana (2007) Proc Natl Acad Sci USA, 104, pp. 18830-18835
  • Zonia, L., Munnik, T., Vesicle trafficking dynamics and visualization of zones of exocytosis and endocytosis in tobacco pollen tubes (2008) J Exp Bot, 59, pp. 861-873

Citas:

---------- APA ----------
Zhang, D., Wengier, D., Shuai, B., Gui, C.-P., Muschietti, J., McCormick, S. & Tang, W.-H. (2008) . The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth. Plant Physiology, 148(3), 1368-1379.
http://dx.doi.org/10.1104/pp.108.124420
---------- CHICAGO ----------
Zhang, D., Wengier, D., Shuai, B., Gui, C.-P., Muschietti, J., McCormick, S., et al. "The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth" . Plant Physiology 148, no. 3 (2008) : 1368-1379.
http://dx.doi.org/10.1104/pp.108.124420
---------- MLA ----------
Zhang, D., Wengier, D., Shuai, B., Gui, C.-P., Muschietti, J., McCormick, S., et al. "The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth" . Plant Physiology, vol. 148, no. 3, 2008, pp. 1368-1379.
http://dx.doi.org/10.1104/pp.108.124420
---------- VANCOUVER ----------
Zhang, D., Wengier, D., Shuai, B., Gui, C.-P., Muschietti, J., McCormick, S., et al. The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth. Plant Physiol. 2008;148(3):1368-1379.
http://dx.doi.org/10.1104/pp.108.124420