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Abstract:

Cellular membranes from 4 to 5-days old etiolated pea seedlings were isolated by isopyenic centrifugation. Marker enzymes were used to measure the purity of the subcellular fractions. The formation of dolichy!-P-glucose and of glucosylated lectin was tested in all the fractions. Both activities were associated with the endoplasmic reticulum fractions. When the membranes were prepared in the presence of EDTA, a shift in both activities to a lower density in the gradient was observed.These results are in agreement with the current assumption that glycosylation of proteins via lipid-linked sugars is a cotranslational process. © 1979 Oxford University Press.

Registro:

Documento: Artículo
Título:Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings
Autor:Hopp, E.H.; Romero, P.; Lezica, R.P.
Filiación:Instituto de Investigaciones Bioquimicas, Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina
Instituto de Investigaciones Bioquimicas, Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina
Fachbereich Biologie, Universitäat Kaiserslautern, Postfach 3049, D-6750 Kaiserslautern, Germany
Palabras clave:Glucosyl transferases; Glycosylation; Lectin biosynthesis; Pisum sativum; Protein
Año:1979
Volumen:20
Número:6
Página de inicio:1063
Página de fin:1069
DOI: http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901
Título revista:Plant and Cell Physiology
Título revista abreviado:Plant Cell Physiol.
ISSN:00320781
CODEN:PCPHA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v20_n6_p1063_Hopp

Referencias:

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Citas:

---------- APA ----------
Hopp, E.H., Romero, P. & Lezica, R.P. (1979) . Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings. Plant and Cell Physiology, 20(6), 1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901
---------- CHICAGO ----------
Hopp, E.H., Romero, P., Lezica, R.P. "Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings" . Plant and Cell Physiology 20, no. 6 (1979) : 1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901
---------- MLA ----------
Hopp, E.H., Romero, P., Lezica, R.P. "Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings" . Plant and Cell Physiology, vol. 20, no. 6, 1979, pp. 1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901
---------- VANCOUVER ----------
Hopp, E.H., Romero, P., Lezica, R.P. Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings. Plant Cell Physiol. 1979;20(6):1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901