Abstract:
Cellular membranes from 4 to 5-days old etiolated pea seedlings were isolated by isopyenic centrifugation. Marker enzymes were used to measure the purity of the subcellular fractions. The formation of dolichy!-P-glucose and of glucosylated lectin was tested in all the fractions. Both activities were associated with the endoplasmic reticulum fractions. When the membranes were prepared in the presence of EDTA, a shift in both activities to a lower density in the gradient was observed.These results are in agreement with the current assumption that glycosylation of proteins via lipid-linked sugars is a cotranslational process. © 1979 Oxford University Press.
Registro:
Documento: |
Artículo
|
Título: | Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings |
Autor: | Hopp, E.H.; Romero, P.; Lezica, R.P. |
Filiación: | Instituto de Investigaciones Bioquimicas, Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina Instituto de Investigaciones Bioquimicas, Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina Fachbereich Biologie, Universitäat Kaiserslautern, Postfach 3049, D-6750 Kaiserslautern, Germany
|
Palabras clave: | Glucosyl transferases; Glycosylation; Lectin biosynthesis; Pisum sativum; Protein |
Año: | 1979
|
Volumen: | 20
|
Número: | 6
|
Página de inicio: | 1063
|
Página de fin: | 1069
|
DOI: |
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901 |
Título revista: | Plant and Cell Physiology
|
Título revista abreviado: | Plant Cell Physiol.
|
ISSN: | 00320781
|
CODEN: | PCPHA
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v20_n6_p1063_Hopp |
Referencias:
- Bowles, D.J., Kauss, H., Carbohydrate-bindingproteinsfrom cellular membranes of plant tissues (1975) Plant Sci. Letters, 4, pp. 411-418
- Bowles, D.J., Kauss, H., Characterization, enzymaticand lectin properties of isolated membranes from (1976) Phaseolus Aureus. Biochim. Biophys. Acta, 443, pp. 360-374
- Bowles, D.J., Schnarrenberger, C., Kauss, H., Lectinsas membrane components of mito chondria from (1976) Ricinus Communis. Biochem. J, 160, pp. 375-382
- Daleo, G.R., Pont Lezica, R., Synthesis of dolichol phosphate by a cell-free extract from pea (1977) Febslett, 74, pp. 247-250
- Depierre, J.W., Dallner, G., Structuralaspectsof the membrane of the endoplasmic re ticulum (1975) Biochim. Biophys. Acta, 425, pp. 411-472
- Donaldson, R.P., Tolbert, N.E., Schnarrenberger, C., (1972) A Comparison of Microbody Membranes with Microsomes and Mitochondria from Plant and Animal Tissue. Arch. Biochem. Biophys, 152, pp. 199-215
- Hemming, F.W., Dolichol phosphate, a coenzyme in the glycosylation of animal membrane- bound glycoproteins (1977) Biochem. Soc. Trans, 5, pp. 1223-1231
- Hopp, H.E., Romero, P.A., Daleo, G.R., Pont Lezica, R., Polyprenylphosphate derivatives as intermediates in the biosynthesis of cellulose precursors. Subcellular localization (1979) Biochemistry and Physiology of Plant Lipids, pp. 313-318. , Edited by L. A. Appelqvist and C. Liljenberg, Elsevier/North Holland Biomedical Press, Amsterdam
- Kauss, H., Plant Lectins (Phytohemagglutinins) (1976) Progress in Botany 38, pp. 58-70. , Edited by H. Ellenberg, K. Esser, H. Merxmuller, E. Schnepf and H. Ziegler, Springer-Verlag, Berlin
- Kauss, H., The possible physiological role of lectins (1977) Cell Wall Biochemistry, pp. 347-359. , Edited by B. Solheim and J. Raa, Universitetsforlaget Oslo, Columbia University Press, New York
- Kauss, H., Bowles, D.J., Someproperties of carbohydrate-binding proteins (Lectins) solubilized from cell walls of (1976) Phaseolus Aureus. Planta, 130, pp. 169-174
- Lehle, L., Bowles, D.J., Tanner, W., Subcellularsite of mannosyl transfer to dolichyl phos phate (1978) Phaseolus Aureus. Plant Sci. Letters, 11, pp. 27-34
- Leonard, R.T., Van Der Woude, W.J., Isolation of plasma membranes from corn roots by sucrose density graident centrifugation: An anomalous effect of Ficoll (1976) Plant Physiol, 57, pp. 105-114
- Lord, J.M., Kagawa, T., Moore, T.S., Beevers, H., Endoplasmicreticulumasthe site of lecithin formation in castor bean endosperm (1973) J. Cell Biol, 57, pp. 659-667
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Proteinmeasurementwith the Folin phenol reagent (1951) J. Biol. Chem, 193, pp. 265-275
- Nagahashi, J., Beevers, L., Subcellular localization of glycosyl transferases involved in glycoprotein biosynthesis in the cotyledons of (1978) Pisum Sativum. Plant Physiol, 61, pp. 451-459
- Pont Lezic, R., Glycosylationofglycoproteinsingreen plants and alga (1979) Biochem. Soc. Trans, pp. 334-433
- Pont Lezica, R., Brett, C.T., Romero Martinez, P., Dankert, M.A., (1975) Biochem. Biophys. Res. Commun, 66, pp. 980-987
- Pont Lezica, R., Romero, P.A., Dankert, M.A., Membrane-bound UDP-glucose: Lipid glucosyltransferases from peas (1976) Plant Physiol, 58, pp. 675-680
- Pont Lezica, R., Romero, P.A., Hopp, H.E., Glucosylation of membrane proteins by lipid- linked glucose (1978) Planta, 140, pp. 177-183
- Ray, P.M., Shininger, T.L., Ray, M.M., Isolation of beta-glucan synthetase particles from plant cells and identification with Golgi membrane (1969) Proc. Nall. Acad. Sci. USA, 64, pp. 605-612
- Romero, P.A., Pont Lezica, R., Transfer of glucose-1-phosphate from UDP-glucose to lipid acceptors in plants (1976) Acta Physiol. Lalinoam, 26, pp. 364-370
- Romero, P.A., Hopp, E., Lezica, R.P., Ont Lezica:Lipid carriers in the synthesis of high mannose glycoproteins in algae Biochim. Biophys. Acta
- Sharon, N., Lis, H., Lectins:Cell-agglutinatingand sugar-specific proteins (1972) Science Ml
- Shore, G., Maclachlan, G.A., Thesite of cellulose synthesis. Hormone treatment alters the intracellular location of alkali-insoluble /?-1, 4-glucan (cellulose) synthetase activity (1957) J. Cell Biol, 64, pp. 557-571
- Staneloni, R.J., Leloir, L.F., Thebiosyntheticpathway of the asparagine-linked oligo saccharides of glycoproteins (1979) TIBS 4, pp. 65-66
- Taussky, H.H., Shorr, E., A microcolorimetric method for the determination of inorganic phosphorus (1953) J. Biol. Chem, 202, pp. 675-685
- Waechter, C.J., Lennarz, W.J., The role of polyprenol-linked sugars in glycoprotein synthesis (1976) Ann. Rev. Biochem, 45, pp. 95-112
Citas:
---------- APA ----------
Hopp, E.H., Romero, P. & Lezica, R.P.
(1979)
. Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings. Plant and Cell Physiology, 20(6), 1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901---------- CHICAGO ----------
Hopp, E.H., Romero, P., Lezica, R.P.
"Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings"
. Plant and Cell Physiology 20, no. 6
(1979) : 1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901---------- MLA ----------
Hopp, E.H., Romero, P., Lezica, R.P.
"Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings"
. Plant and Cell Physiology, vol. 20, no. 6, 1979, pp. 1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901---------- VANCOUVER ----------
Hopp, E.H., Romero, P., Lezica, R.P. Subcellular localization, of glucosyl transferases involved in lectin glucosylation in pisum sativum seedlings. Plant Cell Physiol. 1979;20(6):1063-1069.
http://dx.doi.org/10.1093/oxfordjournals.pcp.a075901