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Abstract:

In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. © 2013 Scandinavian Plant Physiology Society.

Registro:

Documento: Artículo
Título:Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
Autor:Roqueiro, G.; Causin, F.; Olle-Resa, C.; Maroder, H.; Maldonado, S.
Filiación:Estación Experimental Agropecuaria San Juan, INTA, Pocito, San Juan J5427ZAA, Argentina
Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, 6700, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina
Palabras clave:Salix; antioxidant; ascorbate peroxidase; catalase; guaiacol peroxidase; malonaldehyde; peroxidase; reactive oxygen metabolite; superoxide dismutase; analysis of variance; article; chloroplast; cotyledon; germination; growth, development and aging; light; lipid peroxidation; metabolism; oxidation reduction reaction; physiological stress; plant leaf; plant root; plant seed; radiation exposure; seedling; time; transmission electron microscopy; ultrastructure; willow; Analysis of Variance; Antioxidants; Ascorbate Peroxidases; Catalase; Chloroplasts; Cotyledon; Germination; Light; Lipid Peroxidation; Malondialdehyde; Microscopy, Electron, Transmission; Oxidation-Reduction; Peroxidase; Plant Leaves; Plant Roots; Reactive Oxygen Species; Salix; Seedling; Seeds; Stress, Physiological; Superoxide Dismutase; Time Factors
Año:2013
Volumen:149
Número:2
Página de inicio:286
Página de fin:295
DOI: http://dx.doi.org/10.1111/ppl.12048
Título revista:Physiologia Plantarum
Título revista abreviado:Physiol. Plant.
ISSN:00319317
CODEN:PHPLA
CAS:ascorbate peroxidase, 72906-87-7; catalase, 9001-05-2; malonaldehyde, 542-78-9; peroxidase, 9003-99-0; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v149_n2_p286_Roqueiro

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Citas:

---------- APA ----------
Roqueiro, G., Causin, F., Olle-Resa, C., Maroder, H. & Maldonado, S. (2013) . Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination. Physiologia Plantarum, 149(2), 286-295.
http://dx.doi.org/10.1111/ppl.12048
---------- CHICAGO ----------
Roqueiro, G., Causin, F., Olle-Resa, C., Maroder, H., Maldonado, S. "Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination" . Physiologia Plantarum 149, no. 2 (2013) : 286-295.
http://dx.doi.org/10.1111/ppl.12048
---------- MLA ----------
Roqueiro, G., Causin, F., Olle-Resa, C., Maroder, H., Maldonado, S. "Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination" . Physiologia Plantarum, vol. 149, no. 2, 2013, pp. 286-295.
http://dx.doi.org/10.1111/ppl.12048
---------- VANCOUVER ----------
Roqueiro, G., Causin, F., Olle-Resa, C., Maroder, H., Maldonado, S. Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination. Physiol. Plant. 2013;149(2):286-295.
http://dx.doi.org/10.1111/ppl.12048