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Abstract:

The effect of jasmonic acid (JA) on plant growth and on calcium-dependent protein kinase (CDPK) activity and expression was studied in non-photoperiodic potato plants, Solanum tuberosum L. var. Spunta, grown in vitro. Stem cuttings were grown for 45 days (long treatment, LT) in MS medium with increasing concentrations of JA. For short treatments (ST) adult plants grown in MS were transferred for 1, 4 and 20 h to JA containing media. During the LT, low concentrations of JA promoted cell expansion and shoot elongation while higher concentrations caused growth inhibition. Under these conditions, treated plants showed root shortening and tuber formation was not induced. Morphological and histochemical studies using light microscopy and TEM analysis of leaves from treated plants revealed that JA also affected subcellular organelles of mesophyll cells. Peroxisomes increased in size and number, and an autophagic process was triggered in response to high concentrations of the hormone. CDPK activity, determined in crude extracts of treated plants (LT), was inhibited (up to 80%). Plant growth and CDPK inhibition were reverted upon transfer of the plants to hormone-free medium. Soluble CDPK activity decreased in response to JA short treatment. Concomitantly, a decline in the steady state levels of StCDPK2 mRNA, a potato CDPK isoform that is expressed in leaves, was observed. These data suggest that the phytohormone down-regulated the expression and activity of the kinase.

Registro:

Documento: Artículo
Título:Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
Autor:Ulloa, R.M.; Raíces, M.; MacIntosh, G.C.; Maldonado, S.; Téllez-Iñón, M.T.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, United States
Instituto de Tecnología Agropecuaria, INTA-Castelar, Argentina
Palabras clave:Calcium; Cells; Enzymes; Hormones; Optical microscopy; RNA; Tissue; Transmission electron microscopy; Shoot elongation; Plants (botany); Solanum; Solanum tuberosum
Año:2002
Volumen:115
Número:3
Página de inicio:417
Página de fin:427
DOI: http://dx.doi.org/10.1034/j.1399-3054.2002.1150312.x
Título revista:Physiologia Plantarum
Título revista abreviado:Physiol. Plant.
ISSN:00319317
CODEN:PHPLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v115_n3_p417_Ulloa

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Citas:

---------- APA ----------
Ulloa, R.M., Raíces, M., MacIntosh, G.C., Maldonado, S. & Téllez-Iñón, M.T. (2002) . Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum. Physiologia Plantarum, 115(3), 417-427.
http://dx.doi.org/10.1034/j.1399-3054.2002.1150312.x
---------- CHICAGO ----------
Ulloa, R.M., Raíces, M., MacIntosh, G.C., Maldonado, S., Téllez-Iñón, M.T. "Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum" . Physiologia Plantarum 115, no. 3 (2002) : 417-427.
http://dx.doi.org/10.1034/j.1399-3054.2002.1150312.x
---------- MLA ----------
Ulloa, R.M., Raíces, M., MacIntosh, G.C., Maldonado, S., Téllez-Iñón, M.T. "Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum" . Physiologia Plantarum, vol. 115, no. 3, 2002, pp. 417-427.
http://dx.doi.org/10.1034/j.1399-3054.2002.1150312.x
---------- VANCOUVER ----------
Ulloa, R.M., Raíces, M., MacIntosh, G.C., Maldonado, S., Téllez-Iñón, M.T. Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum. Physiol. Plant. 2002;115(3):417-427.
http://dx.doi.org/10.1034/j.1399-3054.2002.1150312.x