Artículo

Juárez, A.B.; Vélez, C.G.; Iñiguez, A.R.; Martínez, D.E.; Rodríguez, M.C.; Vigna, M.S.; De Molina, M.D.C.R. "A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina" (2011) Phycologia. 50(4):413-421
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Abstract:

Acidic geothermal environments are rare and very few organisms can thrive in them. Chlorella or Chlorella-like species have been described in a variety of environments but their presence in extremely acidic waters have rarely been reported. A Chlorella-like alga (strain BAFC CA10) was isolated from Laguna Verde, a volcanic mesothermal acidic pond from northern Patagonia (Argentina). Applying a multimethod approach including microscopic, ultrastructural, chemical and phylogenetic analyses, the acidotolerant strain BAFC CA10 was identified as Parachlorella kessleri (5 Chlorella kessleri) on the basis of comparisons with the authentic strain SAG 211-11g. Our results confirmed the initial characterization of the Laguna Verde strain as C. kessleri, but since this species was transferred to the new genusParachlorella, strain BAFC CA10 should be designated as P. kessleri. Histochemical staining and sugar composition of the cell-wall amorphous and fibrillar matrix, together with transmission electron microscopy of the cells, showed the distinctive characters of the species: (1) glucosamine was the exclusive component of the fibrillar cell wall; (2) rhamnose, galactose, glucose and xylose together with minor quantities of arabinose, mannose and fucose were present in the cellwall hemicellulose matrix; (3) there was a single parietal chloroplast surrounding the entire cell with a small aperture ("mantel-shaped"); (4) there was one pyrenoid in the thickening of the chloroplast surrounded by two starch granules and bisected by two thylakoids; and (5) the cell wall was an electron-transparent homogeneous structure 60-80 nm thick. Phylogenetic analyses of concatenated small-subunit and internal transcribed sequence ribosomal DNA sequences of coccoid green algae within the Chlorella and the Parachlorella clades of the Chlorellaceae confirmed that strain BAFC CA10 is unequivocally related to the P. kessleri strains. Our results suggest that P. kessleri should be added to the list of species capable of adapting to live in extremely acidic environments.

Registro:

Documento: Artículo
Título:A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina
Autor:Juárez, A.B.; Vélez, C.G.; Iñiguez, A.R.; Martínez, D.E.; Rodríguez, M.C.; Vigna, M.S.; De Molina, M.D.C.R.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, pabellón 2, 4u piso. (1428), Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, pabellón 2, 4u piso. (1428), Buenos Aires, Argentina
Department of Biology, Pomona College, Claremont, CA 91711, United States
Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR- CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, pabellón 2, 4u piso. (1428), Buenos Aires, Argentina
Museo Argentino de Ciencias Naturales Bernardino Rivadavia, Av. Á ngel Gallardo 470. (1405), Buenos Aires, Argentina
CONICET, Argentina
Palabras clave:Chlorella kessleri; Chlorellaceae; Geothermal acidic environment; Molecular phylogeny; Parachlorella kessleri; Ultrastructure; algae; Chlorella (Chlorellales); Chlorella (Prasiolales); Chlorella (unclassified Chlorophyceae); Chlorella (unclassified Trebouxiophyceae); Chlorellaceae; Chlorophyta; Coccoidea; Parachlorella; Parachlorella kessleri; Trebouxiophyceae
Año:2011
Volumen:50
Número:4
Página de inicio:413
Página de fin:421
DOI: http://dx.doi.org/10.2216/10-79.1
Título revista:Phycologia
Título revista abreviado:Phycologia
ISSN:00318884
CODEN:PYCOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v50_n4_p413_Juarez

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Citas:

---------- APA ----------
Juárez, A.B., Vélez, C.G., Iñiguez, A.R., Martínez, D.E., Rodríguez, M.C., Vigna, M.S. & De Molina, M.D.C.R. (2011) . A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina. Phycologia, 50(4), 413-421.
http://dx.doi.org/10.2216/10-79.1
---------- CHICAGO ----------
Juárez, A.B., Vélez, C.G., Iñiguez, A.R., Martínez, D.E., Rodríguez, M.C., Vigna, M.S., et al. "A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina" . Phycologia 50, no. 4 (2011) : 413-421.
http://dx.doi.org/10.2216/10-79.1
---------- MLA ----------
Juárez, A.B., Vélez, C.G., Iñiguez, A.R., Martínez, D.E., Rodríguez, M.C., Vigna, M.S., et al. "A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina" . Phycologia, vol. 50, no. 4, 2011, pp. 413-421.
http://dx.doi.org/10.2216/10-79.1
---------- VANCOUVER ----------
Juárez, A.B., Vélez, C.G., Iñiguez, A.R., Martínez, D.E., Rodríguez, M.C., Vigna, M.S., et al. A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina. Phycologia. 2011;50(4):413-421.
http://dx.doi.org/10.2216/10-79.1