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Abstract:

The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl2) in two Chlamydomonas reinhardtii strains: The wild type 4A+ and the pleiotropic mutant cur9, originally selected as a coppertolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mmol m22 s21). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobarbituric acid reactive substances (TBARS) and growth rate, demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72 h incubation. An inverse relation between catalase (CAT) and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as depression in growth rate or increase in TBARS dosage.

Registro:

Documento: Artículo
Título:Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
Autor:Sabatini, S.E.; Leonardi, P.I.; Rodríguez, M.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Piso 4, 1428 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, 1033 Buenos Aires, Argentina
Laboratorio de Ficología y Micología, Departamento de Biología, Universidad Nacional del Sur, San Juan 670, Puerto Rico
CERZOS-CONICET, Camino La Carrindanga Km 7, 8000, Bahía Blanca, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales and CIHIDECAR (CONICET), Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Autotrophic and mixotrophic growth; Catalase; Chlamydomonas reinhardtii; Isocitrate lyase; Lipid peroxidation; Oxidative stress; Chlamydomonadales; Chlamydomonas reinhardtii; Chlorophyceae
Año:2011
Volumen:50
Número:1
Página de inicio:78
Página de fin:88
DOI: http://dx.doi.org/10.2216/09-101.1
Título revista:Phycologia
Título revista abreviado:Phycologia
ISSN:00318884
CODEN:PYCOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v50_n1_p78_Sabatini

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Citas:

---------- APA ----------
Sabatini, S.E., Leonardi, P.I. & Rodríguez, M.C. (2011) . Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions. Phycologia, 50(1), 78-88.
http://dx.doi.org/10.2216/09-101.1
---------- CHICAGO ----------
Sabatini, S.E., Leonardi, P.I., Rodríguez, M.C. "Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions" . Phycologia 50, no. 1 (2011) : 78-88.
http://dx.doi.org/10.2216/09-101.1
---------- MLA ----------
Sabatini, S.E., Leonardi, P.I., Rodríguez, M.C. "Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions" . Phycologia, vol. 50, no. 1, 2011, pp. 78-88.
http://dx.doi.org/10.2216/09-101.1
---------- VANCOUVER ----------
Sabatini, S.E., Leonardi, P.I., Rodríguez, M.C. Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions. Phycologia. 2011;50(1):78-88.
http://dx.doi.org/10.2216/09-101.1