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Abstract:

The effect of chromium on the biology of Euglena gracilis was studied. The ultrastructural modifications caused by this metal and its location within the cell were analyzed by TEM and EDXA, respectively. The effects of chromium on protein, pigment, and lipid contents were also studied in order to evaluate the metabolic responses to metal exposure. Two strains of Euglena gracilis, UTEX 753 (from the Culture Collection of Texas University) and MAT (isolated from the Matanza River), were used in this research. Both were grown in photoauxotrophic and photoheterotrophic conditions and exposed to different metal concentrations. In all treated cells, increases in total protein and lipid contents, changes in chlorophyll amount, and alterations in fine structure were observed, especially with the higher concentration tested. In photosynthetic treated cells, assays showed chloroplast thylakoid disorganization, the presence of cytoplasm lipid globules, and several vacuoles with electron-dense inclusions and remnants of membranes inside. Nuclei presented lobulations, and eventually total fragmentation in some cells treated with the highest chromium concentration was seen, suggesting that chromium cytotoxicity leads to cellular death. The EDXA spectrum showed well-defined Cr and S peaks in the vacuoles containing electron-dense inclusions and remnants of membranes from autotrophic MAT samples. These results indicate that the different defense mechanisms against chromium depend on strain type and culture conditions. The S peak detected in MAT would suggest that sulfur-rich proteins groups play an important role in the detoxification system inducing metal-complex accumulation into vacuoles.

Registro:

Documento: Artículo
Título:Ultrastructure and X-ray microanalysis of Euglena gracilis (Euglenophyta) under chromium stress
Autor:Rocchetta, I.; Leonardi, P.I.; Amado Filho, G.M.; De Molina, M.D.C.R.; Conforti, V.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Cs. Exactas Y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Labotario de Ficología Y Micología, Departamento de Biología, Bioquímica Y Farmacia, CERZOS-CONICET, 8000 Bahía Blanca, Argentina
Instituto de Pesquisas Jardim Botanico/MMA, Jardim Botanico, Rua Pacheco Leao 915, Río de Janeiro, RJ 22460-030, Brazil
Departamento de Química Biológica, Facultad de Cs. Exactas Y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Palabras clave:Chromium; Euglena gracilis; Euglenophyta; Ultrastructure; X-ray microanalysis; Euglena gracilis; Euglenida
Año:2007
Volumen:46
Número:3
Página de inicio:300
Página de fin:306
DOI: http://dx.doi.org/10.2216/06-49.1
Título revista:Phycologia
Título revista abreviado:Phycologia
ISSN:00318884
CODEN:PYCOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v46_n3_p300_Rocchetta

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Citas:

---------- APA ----------
Rocchetta, I., Leonardi, P.I., Amado Filho, G.M., De Molina, M.D.C.R. & Conforti, V. (2007) . Ultrastructure and X-ray microanalysis of Euglena gracilis (Euglenophyta) under chromium stress. Phycologia, 46(3), 300-306.
http://dx.doi.org/10.2216/06-49.1
---------- CHICAGO ----------
Rocchetta, I., Leonardi, P.I., Amado Filho, G.M., De Molina, M.D.C.R., Conforti, V. "Ultrastructure and X-ray microanalysis of Euglena gracilis (Euglenophyta) under chromium stress" . Phycologia 46, no. 3 (2007) : 300-306.
http://dx.doi.org/10.2216/06-49.1
---------- MLA ----------
Rocchetta, I., Leonardi, P.I., Amado Filho, G.M., De Molina, M.D.C.R., Conforti, V. "Ultrastructure and X-ray microanalysis of Euglena gracilis (Euglenophyta) under chromium stress" . Phycologia, vol. 46, no. 3, 2007, pp. 300-306.
http://dx.doi.org/10.2216/06-49.1
---------- VANCOUVER ----------
Rocchetta, I., Leonardi, P.I., Amado Filho, G.M., De Molina, M.D.C.R., Conforti, V. Ultrastructure and X-ray microanalysis of Euglena gracilis (Euglenophyta) under chromium stress. Phycologia. 2007;46(3):300-306.
http://dx.doi.org/10.2216/06-49.1