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Petriella, M.; Deniz, E.; Swaminathan, S.; Roberti, M.J.; Raymo, F.M.; Bossi, M.L. "Superresolution imaging with switchable fluorophores based on oxazine auxochromes" (2013) Photochemistry and Photobiology. 89(6):1391-1398
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Abstract:

The spatial resolution of fluorescence microscopes is limited by diffraction to about half of the light wavelength, hampering the observation of many important intracellular processes. Recent emerging techniques have overcome that diffraction barrier using the temporal discrimination of close objects that are otherwise unresolved or blurred within the spatial resolution of the microscope. The key of these techniques is to switch the signal of fluorescence markers on and off exploiting their distinct molecular states, and detect and localize these markers at the single-molecule level. This underlying principle highlights the critical role of the photophysical properties of the probes, and the importance of finding adequate switching mechanisms. Here, we present strategies to achieve fluorescence modulation based on novel molecular assemblies containing a [1,3]oxazine as the two states, building block responsible for the transformation. Two different triggering events, based on the photochromic and halochromic properties of the oxazine, induce a large absorption and emission bathochromic shift of a pendant fluorophore, as the ultimate fluorescence switching event. The implementation of these approaches to achieve spatial resolution beyond the diffraction limit is also discussed. © 2013 The American Society of Photobiology.

Registro:

Documento: Artículo
Título:Superresolution imaging with switchable fluorophores based on oxazine auxochromes
Autor:Petriella, M.; Deniz, E.; Swaminathan, S.; Roberti, M.J.; Raymo, F.M.; Bossi, M.L.
Filiación:Laboratorio de Nanoscopías Fotónicas, INQUIMAE - DQIAyQF (FCEyN), Universidad de Buenos Aires and Conicet, Buenos Aires, Argentina
Department of Chemistry, Laboratory for Molecular Photonics, University of Miami, Miami, FL, United States
Palabras clave:fluorescent dye; oxazine derivative; Fluorescent Dyes; Oxazines
Año:2013
Volumen:89
Número:6
Página de inicio:1391
Página de fin:1398
DOI: http://dx.doi.org/10.1111/php.12100
Título revista:Photochemistry and Photobiology
Título revista abreviado:Photochem. Photobiol.
ISSN:00318655
CODEN:PHCBA
CAS:Fluorescent Dyes; Oxazines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1391_Petriella

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Citas:

---------- APA ----------
Petriella, M., Deniz, E., Swaminathan, S., Roberti, M.J., Raymo, F.M. & Bossi, M.L. (2013) . Superresolution imaging with switchable fluorophores based on oxazine auxochromes. Photochemistry and Photobiology, 89(6), 1391-1398.
http://dx.doi.org/10.1111/php.12100
---------- CHICAGO ----------
Petriella, M., Deniz, E., Swaminathan, S., Roberti, M.J., Raymo, F.M., Bossi, M.L. "Superresolution imaging with switchable fluorophores based on oxazine auxochromes" . Photochemistry and Photobiology 89, no. 6 (2013) : 1391-1398.
http://dx.doi.org/10.1111/php.12100
---------- MLA ----------
Petriella, M., Deniz, E., Swaminathan, S., Roberti, M.J., Raymo, F.M., Bossi, M.L. "Superresolution imaging with switchable fluorophores based on oxazine auxochromes" . Photochemistry and Photobiology, vol. 89, no. 6, 2013, pp. 1391-1398.
http://dx.doi.org/10.1111/php.12100
---------- VANCOUVER ----------
Petriella, M., Deniz, E., Swaminathan, S., Roberti, M.J., Raymo, F.M., Bossi, M.L. Superresolution imaging with switchable fluorophores based on oxazine auxochromes. Photochem. Photobiol. 2013;89(6):1391-1398.
http://dx.doi.org/10.1111/php.12100