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Abstract:

The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology.

Registro:

Documento: Artículo
Título:Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
Autor:Novo, J.M.; Iriel, A.; Claudia Marchi, M.; Gabriela Lagorio, M.
Filiación:Facultad de Ciencias Exactas y Naturales, INQUIMAE, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Facultad de Ciencias Veterinarias, Centro de Estudios Transdisciplinarios Del Agua, Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Centro de Microscopías Avanzadas, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:Origanum; Origanum vulgare; chemistry; fluorescence microscopy; Origanum; procedures; scanning electron microscopy; spectrofluorometry; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Origanum; Spectrometry, Fluorescence
Año:2013
Volumen:89
Número:6
Página de inicio:1383
Página de fin:1390
DOI: http://dx.doi.org/10.1111/php.12131
Título revista:Photochemistry and Photobiology
Título revista abreviado:Photochem. Photobiol.
ISSN:00318655
CODEN:PHCBA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v89_n6_p1383_Novo

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Citas:

---------- APA ----------
Novo, J.M., Iriel, A., Claudia Marchi, M. & Gabriela Lagorio, M. (2013) . Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment. Photochemistry and Photobiology, 89(6), 1383-1390.
http://dx.doi.org/10.1111/php.12131
---------- CHICAGO ----------
Novo, J.M., Iriel, A., Claudia Marchi, M., Gabriela Lagorio, M. "Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment" . Photochemistry and Photobiology 89, no. 6 (2013) : 1383-1390.
http://dx.doi.org/10.1111/php.12131
---------- MLA ----------
Novo, J.M., Iriel, A., Claudia Marchi, M., Gabriela Lagorio, M. "Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment" . Photochemistry and Photobiology, vol. 89, no. 6, 2013, pp. 1383-1390.
http://dx.doi.org/10.1111/php.12131
---------- VANCOUVER ----------
Novo, J.M., Iriel, A., Claudia Marchi, M., Gabriela Lagorio, M. Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment. Photochem. Photobiol. 2013;89(6):1383-1390.
http://dx.doi.org/10.1111/php.12131