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Abstract:

New photochemical studies of the reactivity of biopterin (BPT) and neopterin (NPT) in acidic (pH = 5.5) and alkaline (pH = 10.5) aqueous solutions at 350 nm and room temperature were performed. The photochemical properties of BPT are of particular interest because the photolysis of this compound takes place in the white skin patches of patients affected by vitiligo. The photochemical reactions were followed by UV/VIS spectrophotometry, HPLC, electrochemical measurement of dissolved O2 and enzymatic methods for hydrogen peroxide (H2O2) and superoxide anion (O 2•-) determinations. When BPT or NPT are exposed to UVA radiation, a red intermediate, very likely 6-formyl-5,8-dihydropterin, is generated in an O2-independent process. That product is rapidly oxidized on admission of O2 to yield 6-formylpterin and H 2O2. When the photolysis takes place in aerobic conditions, no additional pathways exist. On the other hand, in the absence of O2, the intermediate generated is not stable and leads to the formation of many products. O2•- is also generated during photo-oxidation of BPT and NPT. The quantum yields of reactant consumption depends on the O2 concentration: the higher the O 2 concentration, the lower the quantum yields. This behavior is discussed in connection with the excited state of the pterins. © 2008 The Authors.

Registro:

Documento: Artículo
Título:New results on the photochemistry of biopterin and neopterin in aqueous solution
Autor:Vignoni, M.; Cabrerizo, F.M.; Lorente, C.; Thomas, A.H.
Filiación:Instituto de Investigaciones Fisicoquímicas Teóricas Y Aplicadas (INIFTA), CCT-La Plata-CONICET, Universidad Nacional de La Plata, La Plata, Argentina
Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR), Departamento de Química Orgánica, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:biopterin; cytochrome c; neopterin; oxygen; superoxide; article; chemical structure; chemistry; metabolism; photochemistry; solution and solubility; spectrophotometry; Biopterin; Cytochromes c; Molecular Structure; Neopterin; Oxygen; Photochemistry; Solutions; Spectrophotometry; Superoxides
Año:2009
Volumen:85
Número:1
Página de inicio:365
Página de fin:373
DOI: http://dx.doi.org/10.1111/j.1751-1097.2008.00450.x
Título revista:Photochemistry and Photobiology
Título revista abreviado:Photochem. Photobiol.
ISSN:00318655
CODEN:PHCBA
CAS:biopterin, 22150-76-1; cytochrome c, 9007-43-6, 9064-84-0; neopterin, 670-65-5; oxygen, 7782-44-7; superoxide, 11062-77-4; Biopterin, 22150-76-1; Cytochromes c, 9007-43-6; Neopterin, 670-65-5; Oxygen, 7782-44-7; Solutions; Superoxides, 11062-77-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v85_n1_p365_Vignoni

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Citas:

---------- APA ----------
Vignoni, M., Cabrerizo, F.M., Lorente, C. & Thomas, A.H. (2009) . New results on the photochemistry of biopterin and neopterin in aqueous solution. Photochemistry and Photobiology, 85(1), 365-373.
http://dx.doi.org/10.1111/j.1751-1097.2008.00450.x
---------- CHICAGO ----------
Vignoni, M., Cabrerizo, F.M., Lorente, C., Thomas, A.H. "New results on the photochemistry of biopterin and neopterin in aqueous solution" . Photochemistry and Photobiology 85, no. 1 (2009) : 365-373.
http://dx.doi.org/10.1111/j.1751-1097.2008.00450.x
---------- MLA ----------
Vignoni, M., Cabrerizo, F.M., Lorente, C., Thomas, A.H. "New results on the photochemistry of biopterin and neopterin in aqueous solution" . Photochemistry and Photobiology, vol. 85, no. 1, 2009, pp. 365-373.
http://dx.doi.org/10.1111/j.1751-1097.2008.00450.x
---------- VANCOUVER ----------
Vignoni, M., Cabrerizo, F.M., Lorente, C., Thomas, A.H. New results on the photochemistry of biopterin and neopterin in aqueous solution. Photochem. Photobiol. 2009;85(1):365-373.
http://dx.doi.org/10.1111/j.1751-1097.2008.00450.x