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Abstract:

Fluorescence emission from merocyanine 540 (MC540) dimers was observed in dipalmitoylphosphatidylcholine (DPPC) vesicles. This unusual behavior was observed only for vesicles in the gel-phase state. No dimer fluorescence was observed either in monopalmitoylphosphatidylcholine (C16PC) micelles or in liquid-crystalline DPPC vesicles, indicating that dimer fluorescence efficiency increases in highly packed interfaces. The excitonic theory of Kasha was used to interpret the spectral features. The overall fluorescence quantum yield (φφ) decreases with decreasing lipid:probe ratio, not only because of the presence of a weakly fluorescent dimer that absorbs a high fraction of the total absorbed light but also due to quenching of monomer emission. This suggests the existence of probe domains. The dimer fluorescence quantum yields (φφD) were estimated in DPPC large unilamellar vesicles (LUV) and DPPC multilamellar vesicles. The dependence of φφ with probe concentration is compatible with values of φφD lower than 0.05. The dimerization equilibrium of MC540 in C16PC micelles and DPPC-LUV was also studied. Apparent dimerization equilibrium constants, Kdapp and dimer absorption spectrum were calculated in C16PC micelles for the first time. The dimerization equilibrium constant in DPPC-LUV was calculated and discussed in terms of the fraction of volume occupied by the lipid phase.

Registro:

Documento: Artículo
Título:Fluorescent dimers of merocyanine 540 (MC540) in the gel phase of phosphatidylcholine liposomes
Autor:Bernik, D.; Tymczyszyn, E.; Daraio, M.E.; Negri, R.M.
Filiación:Catedra de Quim. Gen. e Inorg., Fac. de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
INQUIMAE, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
INQUIMAE, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Buenos Aires, 1428, Argentina
Año:1999
Volumen:70
Número:1
Página de inicio:40
Página de fin:48
DOI: http://dx.doi.org/10.1111/j.1751-1097.1999.tb01947.x
Título revista:Photochemistry and Photobiology
Título revista abreviado:Photochem. Photobiol.
ISSN:00318655
CODEN:PHCBA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v70_n1_p40_Bernik

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Citas:

---------- APA ----------
Bernik, D., Tymczyszyn, E., Daraio, M.E. & Negri, R.M. (1999) . Fluorescent dimers of merocyanine 540 (MC540) in the gel phase of phosphatidylcholine liposomes. Photochemistry and Photobiology, 70(1), 40-48.
http://dx.doi.org/10.1111/j.1751-1097.1999.tb01947.x
---------- CHICAGO ----------
Bernik, D., Tymczyszyn, E., Daraio, M.E., Negri, R.M. "Fluorescent dimers of merocyanine 540 (MC540) in the gel phase of phosphatidylcholine liposomes" . Photochemistry and Photobiology 70, no. 1 (1999) : 40-48.
http://dx.doi.org/10.1111/j.1751-1097.1999.tb01947.x
---------- MLA ----------
Bernik, D., Tymczyszyn, E., Daraio, M.E., Negri, R.M. "Fluorescent dimers of merocyanine 540 (MC540) in the gel phase of phosphatidylcholine liposomes" . Photochemistry and Photobiology, vol. 70, no. 1, 1999, pp. 40-48.
http://dx.doi.org/10.1111/j.1751-1097.1999.tb01947.x
---------- VANCOUVER ----------
Bernik, D., Tymczyszyn, E., Daraio, M.E., Negri, R.M. Fluorescent dimers of merocyanine 540 (MC540) in the gel phase of phosphatidylcholine liposomes. Photochem. Photobiol. 1999;70(1):40-48.
http://dx.doi.org/10.1111/j.1751-1097.1999.tb01947.x