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Abstract:

Abstract —Human and bovine serum albumin quench enzyme‐generated acetone phosphorescence (Ksv=ca. 104M1). Concomitantly, these proteins are altered as shown by diminished tryptophan absorption at 280 nm, appearance of products of the formylkynurenine type (max=ca. 320 nm) and disappearance of tryptophan fluorescence. These alterations—which are similar to those induced photochemically—were also observed with serum albumins exposed to enzyme‐generated triplet acetaldehyde. On the other hand, triplet acetone generated by the thermolysis of tetramethyldioxetane failed to induce alterations. Presumably energy transfer occurs from the enzyme‐generated triplet species to tryptophan group(s) in the serum albumin associated with the acting enzyme. The detailed mechanism is, however, not yet understood. Copyright © 1984, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:PHOTOCHEMICAL‐LIKE DESTRUCTION OF TRYPTOPHAN IN SERUM ALBUMINS INDUCED BY ENZYME‐GENERATED TRIPLET SPECIES
Autor:Rivas, E.I.; Paladini, A., Jr; Cilento, G.
Filiación:Departamento de Quimica Biológica, Facultad de Farmácia y Bioquimica, Universidad de Buenos Aires, Junin, Buenos Aires, 113, Argentina
Instituto de Ingenieria Genética y Biologia Celular, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, Buenos Aires, 1428, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de Säo Paulo, C.P. 20.780, Säo Paulo, Säo Paulo, Brazil
Palabras clave:acetone; bovine serum albumin; horseradish peroxidase; serum albumin; tryptophan; animal; article; cattle; human; luminescence; metabolism; methodology; spectrofluorometry; Acetone; Animal; Cattle; Horseradish Peroxidase; Human; Luminescence; Serum Albumin; Serum Albumin, Bovine; Spectrometry, Fluorescence; Support, Non-U.S. Gov't; Tryptophan
Año:1984
Volumen:40
Número:5
Página de inicio:565
Página de fin:568
DOI: http://dx.doi.org/10.1111/j.1751-1097.1984.tb05342.x
Título revista:Photochemistry and Photobiology
Título revista abreviado:Photochem. Photobiol.
ISSN:00318655
CAS:acetone, 67-64-1; serum albumin, 9048-46-8; tryptophan, 6912-86-3, 73-22-3; Acetone, 67-64-1; Horseradish Peroxidase, EC 1.11.1.-; Serum Albumin; Serum Albumin, Bovine; Tryptophan, 73-22-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v40_n5_p565_Rivas

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Citas:

---------- APA ----------
Rivas, E.I., Paladini, A., Jr & Cilento, G. (1984) . PHOTOCHEMICAL‐LIKE DESTRUCTION OF TRYPTOPHAN IN SERUM ALBUMINS INDUCED BY ENZYME‐GENERATED TRIPLET SPECIES. Photochemistry and Photobiology, 40(5), 565-568.
http://dx.doi.org/10.1111/j.1751-1097.1984.tb05342.x
---------- CHICAGO ----------
Rivas, E.I., Paladini, A., Jr, Cilento, G. "PHOTOCHEMICAL‐LIKE DESTRUCTION OF TRYPTOPHAN IN SERUM ALBUMINS INDUCED BY ENZYME‐GENERATED TRIPLET SPECIES" . Photochemistry and Photobiology 40, no. 5 (1984) : 565-568.
http://dx.doi.org/10.1111/j.1751-1097.1984.tb05342.x
---------- MLA ----------
Rivas, E.I., Paladini, A., Jr, Cilento, G. "PHOTOCHEMICAL‐LIKE DESTRUCTION OF TRYPTOPHAN IN SERUM ALBUMINS INDUCED BY ENZYME‐GENERATED TRIPLET SPECIES" . Photochemistry and Photobiology, vol. 40, no. 5, 1984, pp. 565-568.
http://dx.doi.org/10.1111/j.1751-1097.1984.tb05342.x
---------- VANCOUVER ----------
Rivas, E.I., Paladini, A., Jr, Cilento, G. PHOTOCHEMICAL‐LIKE DESTRUCTION OF TRYPTOPHAN IN SERUM ALBUMINS INDUCED BY ENZYME‐GENERATED TRIPLET SPECIES. Photochem. Photobiol. 1984;40(5):565-568.
http://dx.doi.org/10.1111/j.1751-1097.1984.tb05342.x