The effect of cycloheximide (2 μg/ml), puromycin (2 μg/ml) and actinomycin D (1 μg/ml), as well as the relation between the influence of cycloheximide and prostaglandin (PG) production on the spontaneous motility of 20 days-ovariectomized rat uterus, were studied. Cycloheximide evoked a more stable motility during the experimental period (180 min) when compared with controls. Neither puromycin nor actinomycin D could madify the spontaneous activity. In the presence of cycloheximide, the rat uterus released more PGE-like material than the untreated one, and no differences were found in the generation of PGF-like material. Indomethacin, acetylsalicylic acid and eicosa-5,8,11, 14- tetraynoic acid could partially block the cycloheximide-induced constancy of motility of uterine strips. Cyproheptadine and atropine had no effect at all. It is suggested that cycloheximide prevents the spontaneoys decay of the contractile activity at least in part by influencing PGE-like material production. © 1982 The Italian Pharmacological Society.
| Documento: | Artículo |
| Título: | Effects of cyclobeximide, puromycin and actinomycin D on the spontaneous motility of isolated rat uterus |
| Autor: | Bonacossa, A.; Franchi, A.M.; Gimeno, M.F.; Seara, S.M.; Gimeno, A.L. |
| Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), the Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET)., 1428 Buenos Aires, Argentina |
| Palabras clave: | 5,8,11,14 icosatetraynoic acid; acetylsalicylic acid; atropine; cycloheptadiene; cycloheximide; cyproheptadine; dactinomycin; indometacin; prostaglandin; puromycin; unclassified drug; animal experiment; article; female genital system; in vitro study; rat; uterus; uterus contractility; Animals; Aspirin; Castration; Cycloheximide; Dactinomycin; Drug Interactions; Female; Prostaglandins; Puromycin; Rats; Rats, Inbred Strains; Time Factors; Uterine Contraction |
| Año: | 1982 |
| Volumen: | 14 |
| Número: | 5 |
| Página de inicio: | 455 |
| Página de fin: | 467 |
| DOI: | http://dx.doi.org/10.1016/S0031-6989(82)80073-8 |
| Título revista: | Pharmacological Research Communications |
| Título revista abreviado: | Pharmacol. Res. Commun. |
| ISSN: | 00316989 |
| CODEN: | PLRCA |
| CAS: | 5,8,11,14 icosatetraynoic acid, 1191-85-1; acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; atropine, 51-55-8, 55-48-1; cycloheptadiene, 29828-02-2; cycloheximide, 642-81-9, 66-81-9; cyproheptadine, 129-03-3, 969-33-5; dactinomycin, 1402-38-6, 1402-58-0, 50-76-0; indometacin, 53-86-1, 74252-25-8, 7681-54-1; puromycin, 53-79-2, 58-58-2; Aspirin, 50-78-2; Cycloheximide, 66-81-9; Dactinomycin, 50-76-0; Prostaglandins; Puromycin, 53-79-2 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00316989_v14_n5_p455_Bonacossa |
