Abstract:
The involvement of the different types of voltage-dependent calcium channels (VDCC) in both DM-BAPTA-AM-incubated and EGTA-AM-incubated mature mice levator auris neuromuscular junctions (NMJ) was studied. We evaluated the effects of ω-agatoxin IVA (ω-Aga IVA), nitrendipine and ω-conotoxin GVIA (ω-CgTX) (P/Q-, L- and N-type VDCC blockers, respectively) on perineurial calcium currents (ICa) and nerve-evoked transmitter release. The application of ω-Aga IVA (100 nM) drastically reduced perineurial ICa (> 90%) and nerve-evoked transmitter release (> 90% of reduction in quantal content, m) at both DM-BAPTA-AM-incubated and EGTA-AM-incubated NMJ. The L-type VDCC antagonist nitrendipine (10 μM) caused a significant reduction (23 ± 9%, n = 5) of perineurial ICa at DM-BAPTA-AM-incubated NMJ. In addition, after the block of P/Q-type VDCC with ω-Aga IVA (100 nM), nitrendipine reduced (> 90%, n = 2) the remaining perineurial ICa. Such reduction was not observed at EGTA-AM-incubated NMJ, before or after the total block of P/Q-type VDCC. Moreover, nitrendipine did not significantly reduce the quantal content of DM-BAPTA-AM-incubated NMJ. Finally, the application of ω-CgTX (5 μM) did not significantly affect perineurial ICa or nerve-evoked transmitter release at either DM-BAPTA-AM-incubated or EGTA-AM-incubated NMJ. These results show the existence of a nitrendipine-sensitive, L-type component of perineurial ICa in DM-BAPTA-AM-incubated NMJ of mature mice. © Springer-Verlag 1999.
Registro:
Documento: |
Artículo
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Título: | L-type calcium channels unmasked by cell-permeant Ca2+ buffer at mouse motor nerve terminals |
Autor: | Urbano, F.J.; Uchitel, O.D. |
Filiación: | Laboratorio de Fisiología y Biología Molecular (LFBM), Departamento de Ciencias Biológicas, Pebellón II, 1428-Buenos Aires, Argentina Laboratorio de Fisiología y Biología Molecular (LFBM), Departamento de Ciencias Biológicas, Ciudad Universitaria, 1428-Buenos Aires, Argentina
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Palabras clave: | Calcium channel; Cell-permeant calcium buffers; DM-BAPTA-AM; EGTA-AM; Neuromuscular junction; calcium; calcium channel; calcium channel blocking agent; animal cell; animal experiment; animal tissue; article; calcium current; calcium transport; electrophysiology; male; mouse; nerve ending; nerve potential; neuromuscular synapse; neurotransmission; neurotransmitter release; nonhuman; priority journal; Animals; Buffers; Calcium; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Chelating Agents; Egtazic Acid; Electric Conductivity; Evoked Potentials; Male; Mice; Motor Endplate; Nitrendipine; omega-Agatoxin IVA; omega-Conotoxin GVIA; Peptides; Spider Venoms |
Año: | 1999
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Volumen: | 437
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Número: | 4
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Página de inicio: | 523
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Página de fin: | 528
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DOI: |
http://dx.doi.org/10.1007/s004240050813 |
Título revista: | Pflugers Archiv European Journal of Physiology
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Título revista abreviado: | Pflug. Arch. Eur. J. Physiol.
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ISSN: | 00316768
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CODEN: | PFLAB
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CAS: | 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, 85233-19-8; Buffers; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Calcium, 7440-70-2; Chelating Agents; Egtazic Acid, 67-42-5; Nitrendipine, 39562-70-4; omega-Agatoxin IVA; omega-Conotoxin GVIA, 92078-76-7; Peptides; Spider Venoms
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00316768_v437_n4_p523_Urbano |
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Citas:
---------- APA ----------
Urbano, F.J. & Uchitel, O.D.
(1999)
. L-type calcium channels unmasked by cell-permeant Ca2+ buffer at mouse motor nerve terminals. Pflugers Archiv European Journal of Physiology, 437(4), 523-528.
http://dx.doi.org/10.1007/s004240050813---------- CHICAGO ----------
Urbano, F.J., Uchitel, O.D.
"L-type calcium channels unmasked by cell-permeant Ca2+ buffer at mouse motor nerve terminals"
. Pflugers Archiv European Journal of Physiology 437, no. 4
(1999) : 523-528.
http://dx.doi.org/10.1007/s004240050813---------- MLA ----------
Urbano, F.J., Uchitel, O.D.
"L-type calcium channels unmasked by cell-permeant Ca2+ buffer at mouse motor nerve terminals"
. Pflugers Archiv European Journal of Physiology, vol. 437, no. 4, 1999, pp. 523-528.
http://dx.doi.org/10.1007/s004240050813---------- VANCOUVER ----------
Urbano, F.J., Uchitel, O.D. L-type calcium channels unmasked by cell-permeant Ca2+ buffer at mouse motor nerve terminals. Pflug. Arch. Eur. J. Physiol. 1999;437(4):523-528.
http://dx.doi.org/10.1007/s004240050813