Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Arbuscular mycorrhizal (AM) fungi and phosphate solubilizing bacteria (PSB) have a positive effect on plant productivity primarily through increasing phosphate availability. In order to study the interaction between AM fungi and PSB, we used . Bacillus megaterium, a PSB isolated from the sterilized surface of AM germinated spores, and two strains of the AM fungus . Glomus intraradices with different mycelial architecture. A greenhouse experiment was designed with maize as host plant with the addition of tribasic calcium phosphate. We tested the hypothesis that PSB, intimately linked with AM fungi, could interact differentially with the two AM strains. We concluded that inoculation with the PSB positively affected maize mycorrhization. Insoluble phosphate alone did not influence the AM extraradical mycelium (ERM) length and maize mycorrhization when bacteria were not inoculated. The results provide evidence that the adverse effect on infectivity for some AM strains might be caused by solubilized phosphorus release to the rhizosphere by PSB. Differences related to the mycelium architecture of each AM strain were observed: the density of PSB in rhizosphere soil was significantly higher only with the GA8 strain coinciding with the highest values of maize biomass. The density of bacteria associated with GA8 mycelium could be the result of the transfer of photosynthates through the rhizosphere; this close contact would favor the persistence of the intimate relationship between PSB and AM hyphae. In the bacteria-free treatments, soil adherence was not significantly altered. Although the highest development of ERM occurred with GA5, plants inoculated with GA8 showed the highest values for soil adherence. This may be due to the AM mycelium which modifies bacterial persistence in the rhizosphere and consequently soil adherence. Our results show that for potential applications, some characteristics of the AM strains are key in the selection of the AM fungi-PSB combinations. These include the tolerance to soluble phosphorus, the rate of root colonization, and ERM development that favors the persistence of bacteria in rhizosphere soil. © 2012 Elsevier GmbH.

Registro:

Documento: Artículo
Título:Differential interaction between two Glomus intraradices strains and a phosphate solubilizing bacterium in maize rhizosphere
Autor:Fernández Bidondo, L.; Bompadre, J.; Pergola, M.; Silvani, V.; Colombo, R.; Bracamonte, F.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to piso, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Bacillus megaterium; Extraradical-mycelium growth patterns; Glomus intraradices; Microbial interactions; arbuscular mycorrhiza; bacterium; biological production; fungus; greenhouse ecosystem; growth rate; host plant; hypothesis testing; inoculation; interspecific interaction; maize; nutrient availability; persistence; phosphate; phytomass; rhizosphere; root colonization; spore; Arbuscular; Bacillus megaterium; Bacteria (microorganisms); Fungi; Glomus; Glomus intraradices; Zea mays
Año:2012
Volumen:55
Número:4
Página de inicio:227
Página de fin:232
DOI: http://dx.doi.org/10.1016/j.pedobi.2012.04.001
Título revista:Pedobiologia
Título revista abreviado:Pedobiologia
ISSN:00314056
CODEN:PDBLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00314056_v55_n4_p227_FernandezBidondo

Referencias:

  • Andrade, G., Mihara, K.L., Linderman, R.G., Bethlenfalvay, G.J., Bacteria from rhizosphere and hyphosphere soils of different arbuscular mycorrhizal fungi (1997) Plant and Soil, 192, pp. 71-79
  • Artursson, V., Finlay, R.D., Jansson, J.K., Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth (2006) Environmental Microbiology, 8, pp. 1-10
  • Barea, J.M., Mycorrhiza-bacteria interactions on plant growth promotion (1997) Plant Growth Promoting Rhizobacteria, pp. 150-158. , OECD Press, Paris, France, A. Ogoshi, K. Kobayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (Eds.)
  • Bécard, G., Fortin, A., Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots (1988) New Phytologist, 108, pp. 211-218
  • Boomsma, C.R., Vyn, T.J., Maize drought tolerance: potential improvements through arbuscular mycorrhizal symbiosis? (2008) Field Crops Research, 108, pp. 14-31
  • Brundrett, M., Melville, L., Peterson, L., Extraction and staining of hyphae from soil (1994) Practical Methods in Mycorrhiza Research, pp. 71-80. , Mycologue Publications, Waterloo, M. Brundrett, L. Melville, L. Peterson (Eds.)
  • Chiou, T.J., Liu, H., Harrison, M.J., The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface (2001) The Plant Journal, 25 (3), pp. 281-293
  • Clewer, A.G., Scarisbrick, D.H., Factorial experiments (2001) Practical Statistics and Experimental Design for Plant and Crop Science, pp. 159-181. , J. Wiley and Sons, Chichester, England
  • De Leij, F.A.A.M., Whipps, J.M., Lynch, J.M., The use of colony development for the characterization of bacterial communities in soil and on roots (1993) Microbial Ecology, 27, pp. 81-97
  • Declerck, S., D'Or, D., Bivort, C., de Souza, F.A., Development of extraradical mycelium of Scutellospora reticulata under root-organ culture: spore production and function of auxiliary cells (2004) Mycological Research, 108, pp. 84-92
  • Frey-Klett, P., Churin, J.L., Pierrat, J.C., Garbaye, J., Dose effect in the dual inoculation of an ectomycorrhizal fungus and a mycorrhiza helper bacterium in two forest nurseries (1999) Soil Biology & Biochemistry, 31, pp. 1555-1562
  • Frey-Klett, P., Garbaye, J., Tarkka, M., The mycorrhiza helper bacteria revisited (2007) New Phytologist, 176, pp. 22-36
  • Friese, C.F., Allen, M.F., The spread of VA mycorrhizal fungal hyphae in the soil: inoculum types and external hyphal architecture (1991) Mycologia, 83 (4), pp. 409-418
  • Gerhardson, B., Clarholm, M., Microbial communities on plant roots (1986) Microbial Communities in Soil, pp. 19-34. , Elsevier, London, V. Jensen, A. Kjöller, L.H. Sörensen (Eds.)
  • Graham, J.H., Linderman, R.G., Menge, J.A., Development of external hyphae by different isolates of mycorrhizal Glomus spp. in relation to root colonization and growth of troyer citrange (1982) New Phytologist, 91, pp. 183-189
  • Gryndler, M., Interactions of arbuscular mycorrhizal fungi with other soil organisms (2000) Arbuscular Mycorrhizas: Physiology and Function, pp. 239-262. , Kluwer Academic Publishers, Netherlands, Y. Kapulnik, Douds, D.D. Dordrecht (Eds.)
  • Hewitt, E.J., Sand water culture methods used in the study of plant nutrition. Commonwealth Agriculture Bureau (1952), Technical Communication No. 22; Hinsinger, P., Bengough, A.G., Vetterlein, D., Young, I.M., Rhizosphere: biophysics, biogeochemistry and ecological relevance (2009) Plant and Soil, 321, pp. 117-152
  • Jastrow, J.D., Miller, R.M., Lussenhop, J., Contributions of interacting biological mechanisms to soil aggregate stabilization in restored prairie (1998) Soil Biology & Biochemistry, 30, pp. 905-916
  • Kaci, Y., Heyraund, A., Barakat, M., Heulin, T., Isolation and identification of an EPS-producing Rhizobium strain from arid soil (Algeria): characterisation of its EPS and the effect of inoculation on wheat rhizosphere soil structure (2005) Research in Microbiology, 156, pp. 522-531
  • Kim, K.Y., Jordan, D., McDonald, G.A., Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity (1998) Biology and Fertility of Soils, 26, pp. 79-87
  • Kohler, J., Caravaca, F., Carrasco, L., Roldán, A., Contribution of Pseudomonas mendocina and Glomus intraradices to aggregate stabilization and promotion of biological fertility in rhizosphere soil of lettuce plants under field conditions (2006) Soil Use and Management, 22 (3), pp. 298-304
  • Liu, A., Hamel, C., Hamilton, R.I., Ma, B.L., Smith, D.L., Acquisition of Cu, Zn, Mn and Fe by mycorrhizal maize (Zea mays L.) grown in soil at different P and micronutrient levels (2000) Mycorrhiza, 9, pp. 331-336
  • Lynch, J.M., Whipps, J.M., Substrate flow in the rhizosphere (1991) Plant and Soil, 129, pp. 1-10
  • Maldonado-Mendoza, I.E., Dewbre, G.R., Harrison, M.J., A phosphate transporter gene from the extra-radical mycelium of an arbuscular mycorrhizal fungus Glomus intraradices is regulated in response to phosphate in the environment (2001) Molecular Plant-Microbe Interactions, 14 (10), pp. 1140-1148
  • Marschner, P., Timonen, S., Interactions between plant species and mycorrhizal colonization on the bacterial community composition in the rhizosphere (2005) Applied Soil Ecology, 28, pp. 23-36
  • Marulanda, A., Azcón, R., Ruíz-Lozano, J.M., Aroca, R., Differential effects of a Bacillus megaterium strain on Lactuca sativa plant growth depending on the origin of the arbuscular mycorrhizal fungus coinoculated: physiologic and biochemical traits (2008) Journal of Plant Growth Regulation, 27, pp. 10-18
  • Nautiyal, C.S., An efficient microbiological growth medium for screening phosphate solubilizing microorganisms (1999) FEMS Microbiology Letters, 170, pp. 265-270
  • Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Transactions of the British Mycological Society, 55, pp. 158-160
  • Silvani, V., Fracchia, S., Fernández, L., Pérgola, M., Godeas, A., A simple method to obtain endophytic microorganism from field collected roots (2008) Soil Biology & Biochemistry, 40, pp. 1259-1263
  • Silvani, V.A., Aislamiento y Caracterización in vitro de hongos micorrícicos Arbusculares de diferentes sitios en Argentina (2011), Ph.D. Thesis. FCEyN. UBA; Smith, S.E., Read, D.J., (1997) Mycorrhizal Symbiosis, p. 605. , Academic Press, London
  • Toro, M., Azcón, R., Barea, J.M., Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate-solubilizing rhizobacteria to improve rock phosphate bioavailability ( 32P) and nutrient cycling (1997) Applied and Environment Microbiology, 63, pp. 4408-4412
  • Vivas, A., Marulanda, A., Ruiz-Lozano, J.M., Barea, J.M., Azcón, R., Influence of a Bacillus sp. on physiological activities of two arbuscular mycorrhizal fungi and on plant responses to PEG-induced drought stress (2003) Mycorrhiza, 13, pp. 249-256
  • Watt, M., McCully, M.E., Canny, M.J., Formation and stabilization of maize rhizosheaths: effect of soil water content (1994) Plant Physiology, 106, pp. 179-186
  • Wu, S.C., Caob, Z.H., Lib, Z.G., Cheunga, K.C., Wonga, M.H., Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial (2005) Geoderma, 125, pp. 155-166

Citas:

---------- APA ----------
Fernández Bidondo, L., Bompadre, J., Pergola, M., Silvani, V., Colombo, R., Bracamonte, F. & Godeas, A. (2012) . Differential interaction between two Glomus intraradices strains and a phosphate solubilizing bacterium in maize rhizosphere. Pedobiologia, 55(4), 227-232.
http://dx.doi.org/10.1016/j.pedobi.2012.04.001
---------- CHICAGO ----------
Fernández Bidondo, L., Bompadre, J., Pergola, M., Silvani, V., Colombo, R., Bracamonte, F., et al. "Differential interaction between two Glomus intraradices strains and a phosphate solubilizing bacterium in maize rhizosphere" . Pedobiologia 55, no. 4 (2012) : 227-232.
http://dx.doi.org/10.1016/j.pedobi.2012.04.001
---------- MLA ----------
Fernández Bidondo, L., Bompadre, J., Pergola, M., Silvani, V., Colombo, R., Bracamonte, F., et al. "Differential interaction between two Glomus intraradices strains and a phosphate solubilizing bacterium in maize rhizosphere" . Pedobiologia, vol. 55, no. 4, 2012, pp. 227-232.
http://dx.doi.org/10.1016/j.pedobi.2012.04.001
---------- VANCOUVER ----------
Fernández Bidondo, L., Bompadre, J., Pergola, M., Silvani, V., Colombo, R., Bracamonte, F., et al. Differential interaction between two Glomus intraradices strains and a phosphate solubilizing bacterium in maize rhizosphere. Pedobiologia. 2012;55(4):227-232.
http://dx.doi.org/10.1016/j.pedobi.2012.04.001