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Abstract:

Establishing the putative links between sylvatic and domestic transmission cycles of Trypanosoma cruzi, the etiological agent of Chagas disease, is of public health relevance. We conducted three surveys to assess T. cruzi infection in wild mammals from a rural and a preserved area in Misiones Province, Northeastern Argentina, which had recently been declared free of vector- and blood-borne transmission of human T. cruzi infection. A total of 200 wild mammals were examined by xenodiagnosis (XD) and/or polymerase chain reaction (PCR) amplification of the hyper-variable region of kinetoplast DNA minicircles of T. cruzi (kDNA-PCR). The overall prevalence of T. cruzi infection was 8%. Nine (16%) of 57 Didelphis albiventris opossums and two (7%) of 29 Desmodus rotundus vampire bats were positive by both XD and kDNA-PCR. Additionally, one D. rotundus positive for T. cruzi by kDNA-PCR tested positive by satellite-DNA-PCR (SAT-DNA-PCR). The T. cruzi-infected bats were captured indoors and in the yard of a vacant dwelling. All D. albiventris were infected with TcI and both XD-positive D. rotundus by TcII. Fifty-five opossum cubs within the marsupium were negative by XD. The mean infectiousness to the vector was 62% in D. albiventris and 50% in D. rotundus. Mice experimentally infected with a parasite isolate from a vampire bat displayed lesions typically caused by T. cruzi. Our study documents the presence of the genotype TcII in a sylvatic host for the first time in Argentina, and the occurrence of two transmission cycles of T. cruzi in a district free of domestic vector-borne transmission. © Cambridge University Press 2016.

Registro:

Documento: Artículo
Título:First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina
Autor:Argibay, H.D.; Orozco, M.M.; Cardinal, M.V.; Rinas, M.A.; Arnaiz, M.; Mena Segura, C.; Gürtler, R.E.
Filiación:Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, UBA-IEGEBA CONICET, Buenos Aires, Argentina
Parque Ecológico El Puma, Ministerio de Ecología y Recursos Naturales Renovables, Provincia de Misiones, Argentina
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, ANLIS Malbrán, Buenos Aires, Argentina
Instituto de Zoonosis Luis Pasteur, Gobierno de la Ciudad de Buenos Aires, Argentina
Palabras clave:Desmodus rotundus; Didelphis albiventris; discrete typing unit; Trypanosoma cruzi; kinetoplast DNA; minicircle DNA; protozoal DNA; animal experiment; animal model; animal tissue; Argentina; Article; Chagas disease; cub; Desmodus rotundus; Didelphis albiventris; disease transmission; gene amplification; molecular diagnosis; mouse; nonhuman; nucleotide sequence; parasite isolation; polymerase chain reaction; priority journal; Trypanosoma cruzi; wild animal; xenodiagnosis; animal; bat; Chagas disease; disease carrier; genetics; isolation and purification; mammal; opossum; parasitology; prevalence; transmission; veterinary; Animals; Animals, Wild; Argentina; Chagas Disease; Chiroptera; Disease Reservoirs; Disease Vectors; DNA, Protozoan; Mammals; Opossums; Polymerase Chain Reaction; Prevalence; Trypanosoma cruzi; Xenodiagnosis
Año:2016
Volumen:143
Número:11
Página de inicio:1358
Página de fin:1368
DOI: http://dx.doi.org/10.1017/S0031182016000925
Título revista:Parasitology
Título revista abreviado:Parasitology
ISSN:00311820
CODEN:PARAA
CAS:DNA, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v143_n11_p1358_Argibay

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Citas:

---------- APA ----------
Argibay, H.D., Orozco, M.M., Cardinal, M.V., Rinas, M.A., Arnaiz, M., Mena Segura, C. & Gürtler, R.E. (2016) . First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina. Parasitology, 143(11), 1358-1368.
http://dx.doi.org/10.1017/S0031182016000925
---------- CHICAGO ----------
Argibay, H.D., Orozco, M.M., Cardinal, M.V., Rinas, M.A., Arnaiz, M., Mena Segura, C., et al. "First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina" . Parasitology 143, no. 11 (2016) : 1358-1368.
http://dx.doi.org/10.1017/S0031182016000925
---------- MLA ----------
Argibay, H.D., Orozco, M.M., Cardinal, M.V., Rinas, M.A., Arnaiz, M., Mena Segura, C., et al. "First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina" . Parasitology, vol. 143, no. 11, 2016, pp. 1358-1368.
http://dx.doi.org/10.1017/S0031182016000925
---------- VANCOUVER ----------
Argibay, H.D., Orozco, M.M., Cardinal, M.V., Rinas, M.A., Arnaiz, M., Mena Segura, C., et al. First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina. Parasitology. 2016;143(11):1358-1368.
http://dx.doi.org/10.1017/S0031182016000925