Abstract:
Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population. © Copyright 2012 Published by Cambridge University Press.
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Documento: |
Artículo
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Título: | Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina |
Autor: | Cura, C.I.; Lucero, R.H.; Bisio, M.; Oshiro, E.; Formichelli, L.B.; Burgos, J.M.; Lejona, S.; Brusés, B.L.; Hernández, D.O.; Severini, G.V.; Velazquez, E.; Duffy, T.; Anchart, E.; Lattes, R.; Altcheh, J.; Freilij, H.; Diez, M.; Nagel, C.; Vigliano, C.; Favaloro, L.; Favaloro, R.R.; Merino, D.E.; Sosa-Estani, S.; Schijman, A.G. |
Filiación: | Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina Instituto de Medicina Regional, Universidad Nacional Del Nordeste, Av. Las Heras 727, 3500, Resistencia, Chaco, Argentina Centro Nacional de Diagnóstico e Investigación de Endemo-epidemias (CeNDIE), ANLIS Malbrán, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina Departamento de Microbiología, Inmunología y Parasitología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Ciudad de Buenos Aires, Argentina Área de Biología Molecular, Centro de Especialidades Médicas Ambulatorias (CEMAR), San Luis 2020, 2000, Rosario, Santa Fe, Argentina Consultorio de Chagas, Facultad de Medicina, Universidad Nacional Del Nordeste, Moreno 1240, 3400, Corrientes, Argentina Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina Instituto de Nefrología, Cabello 3889, 1425, Ciudad de Buenos Aires, Argentina Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Gallo 1330, 1425, Buenos Aires, Argentina Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
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Palabras clave: | Chagas disease; Discrete Typing Unit; PCR; Trypanosoma cruzi; adolescent; adult; aged; Argentina; article; bloodstream infection; Chagas disease; child; chronicity; endemic disease; geographic distribution; graft recipient; human; human tissue; immunocompromised patient; major clinical study; pathogenesis; polymerase chain reaction; priority journal; skin biopsy; taxonomy; tissue distribution; Trypanosoma cruzi; Adolescent; Adult; Aged; Aged, 80 and over; Argentina; Chagas Cardiomyopathy; Chagas Disease; Child; Child, Preschool; DNA, Protozoan; Endemic Diseases; Female; Genetic Variation; Genotype; Heart; Humans; Infant; Male; Middle Aged; Phylogeny; Polymerase Chain Reaction; Trypanosoma cruzi; Young Adult; Trypanosoma cruzi |
Año: | 2012
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Volumen: | 139
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Número: | 4
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Página de inicio: | 516
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Página de fin: | 521
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DOI: |
http://dx.doi.org/10.1017/S0031182011002186 |
Título revista: | Parasitology
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Título revista abreviado: | Parasitology
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ISSN: | 00311820
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CODEN: | PARAA
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CAS: | DNA, Protozoan
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v139_n4_p516_Cura |
Referencias:
- Bisio, M., Cura, C., Duffy, T., Altcheh, J., Giganti, S.O., Begher, S., Scapellato, P., Schijman, A.G., Trypanosoma cruzi discrete typing units in chagas disease patients with hiv co-infection (2009) Revista Biomédica, 20, pp. 166-178
- Brenière, S.F., Bosseno, M.F., Telleria, J., Bastrenta, B., Yacsik, N., Noireau, F., Alcazar, J.L., Tibayrenc, M., Different behavior of two trypanosoma cruzi major clones: Transmission and circulation in young bolivian patients (1998) Experimental Parasitology, 89, pp. 285-295
- Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M., Seidenstein, M.E., Piccinali, R., Schijman, A.G., Direct molecular characterization of bloodstream parasite populations causing congenital chagas disease (2007) International Journal for Parasitology, 37, pp. 1319-1327
- Burgos, J.M., Begher, S.B., Freitas, J.M., Bisio, M., Duffy, T., Altcheh, J., Teijeiro, R., Schijman, A.G., Molecular diagnosis and typing of trypanosoma cruzi populations and lineages in cerebral chagas disease in a patient with aids (2005) The American Journal of Tropical Medicine and Hygiene, 73, pp. 1016-1018
- Burgos, J.M., Diez, M., Vigliano, C., Bisio, M., Risso, M., Duffy, T., Cura, C., Schijman, A.G., Molecular identification of trypanosoma cruzi discrete typing units in end-stage chronic chagas heart disease and reactivation after heart transplantation (2010) Clinical Infectious Diseases, 51, pp. 485-495
- Campbell, D.A., Westenberger, S.J., Sturm, N.R., The determinants of chagas disease: Connecting parasite and host genetics (2004) Current Molecular Medicine, 4, pp. 549-562
- Carranza, J.C., Valadares, H.M., D'Avila, D.A., Baptista, R.P., Moreno, M., Galvão, L.M., Chiari, E., Zingales, B., Trypanosoma cruzi maxicircle heterogeneity in chagas disease patients from brazil (2009) International Journal for Parasitology, 39, pp. 963-973
- Di Noia, J.M., Buscaglia, C.A., De Marchi, C.R., Almeida, I.C., Frasch, A.C., A trypanosoma cruzi small surface molecule provides the first immunological evidence that chagas' disease is due to a single parasite lineage (2002) The Journal of Experimental Medicine, 195, pp. 401-413
- Diosque, P., Barnabe, C., Padilla, A., Marco, J., Cardozo, R., Cimino, R., Nasserd, J., Basombrio, M., Multilocus enzyme electrophoresis analysis of trypanosoma cruzi isolates from a geographically restricted endemic area for chagas' disease in argentina (2003) International Journal for Parasitology, 33, pp. 997-1003
- Duffy, T., Bisio, M., Altcheh, J., Burgos, J.M., Diez, M., Levin, M.J., Favaloro, R.R., Schijman, A.G., Accurate real-time pcr strategy for monitoring bloodstream parasitic loads in chagas disease patients (2009) PLoS Neglected Tropical Diseases, 3, pp. e419
- Freitas, J.M., Lages-Silva, E., Crema, E., Pena, S.D., Macedo, A.M., Real time pcr strategy for the identification of major lineages of trypanosoma cruzi directly in chronically infected human tissues (2005) International Journal for Parasitology, 35, pp. 411-417
- Gaunt, M.W., Yeo, M., Frame, I.A., Stothard, J.R., Carrasco, H.J., Taylor, M.C., Mena, S.S., Miles, M.A., Mechanism of genetic exchange in american trypanosomes. Nature (2003) London, 421, pp. 936-939
- Higo, H., Miura, S., Horio, M., Mimori, T., Hamano, S., Agatsuma, T., Yanagi, T., Himeno, K., Genotypic variation among lineages of trypanosoma cruzi and its geographic aspects (2004) Parasitology International, 53, pp. 337-344
- Macedo, A.M., Pena, S.D.J., Genetic variability of trypanosoma cruzi: Implications for the pathogenesis of chagas disease (1998) Parasitology Today, 14, pp. 119-123
- Macedo, A.M., Machado, C.R., Oliveira, R.P., Pena, S.D., Trypanosoma cruzi: Genetic structure of populations and relevance of genetic variability to the pathogenesis of chagas disease (2004) Memórias do Instituto Oswaldo Cruz, 99, pp. 1-12
- Miles, M.A., Llewellyn, M.S., Lewis, M.D., Yeo, M., Baleela, R., Fitzpatrick, S., Gaunt, M.W., Mauricio, I.L., The molecular epidemiology and phylogeography of trypanosoma cruzi and parallel research on leishmania: Looking back and to the future (2009) Parasitology, 136, pp. 1509-1528
- Ocaña-Mayorga, S., Llewellyn, M.S., Costales, J.A., Miles, M.A., Grijalva, M.J., Sex, subdivision, and domestic dispersal of trypanosoma cruzi lineage i in southern ecuador (2010) PLoS Neglected Tropical Diseases, 4, pp. e915
- Vago, A.R., Andrade, L.O., Leite, A.A., D'Avila Reis, D., Macedo, A.M.S.J., Tostes Jr., S., Moreira, M.C., Pena, S.D., Genetic characterization of trypanosoma cruzi directly from tissues of patients with chronic chagas disease: Differential distribution of genetic types into diverse organs (2000) The American Journal of Pathology, 156, pp. 1805-1809
- Virreira, M., Alonso-Vega, C., Solano, M., Jijena, J., Brutus, L., Bustamante, Z., Truyens, C., Svoboda, M., Congenital chagas disease in bolivia is not associated with dna polymorphism of trypanosoma cruzi (2006) The American Journal of Tropical Medicine and Hygiene, 75, pp. 871-879
- (2007) Report of the Scientific Working Group on Chagas Disease, , World Health Organization, TDR/SWG/09.World Health Organization, Geneva, Switzerland
- Zingales, B., Andrade, S.G., Briones, M.R., Campbell, D.A., Chiari, E., Fernandes, O., Guhl, F., Schijman, A.G., A new consensus for trypanosoma cruzi intraspecific nomenclature: Second revision meeting recommends tci to tcvi (2009) Memórias do Instituto Oswaldo Cruz, 104, pp. 1051-1054
Citas:
---------- APA ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., Lejona, S.,..., Schijman, A.G.
(2012)
. Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina. Parasitology, 139(4), 516-521.
http://dx.doi.org/10.1017/S0031182011002186---------- CHICAGO ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., et al.
"Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina"
. Parasitology 139, no. 4
(2012) : 516-521.
http://dx.doi.org/10.1017/S0031182011002186---------- MLA ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., et al.
"Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina"
. Parasitology, vol. 139, no. 4, 2012, pp. 516-521.
http://dx.doi.org/10.1017/S0031182011002186---------- VANCOUVER ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., et al. Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina. Parasitology. 2012;139(4):516-521.
http://dx.doi.org/10.1017/S0031182011002186