Artículo

Cura, C.I.; Lucero, R.H.; Bisio, M.; Oshiro, E.; Formichelli, L.B.; Burgos, J.M.; Lejona, S.; Brusés, B.L.; Hernández, D.O.; Severini, G.V.; Velazquez, E.; Duffy, T.; Anchart, E.; Lattes, R.; Altcheh, J.; Freilij, H.; Diez, M.; Nagel, C. (...) Schijman, A.G. "Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina" (2012) Parasitology. 139(4):516-521
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Abstract:

Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population. © Copyright 2012 Published by Cambridge University Press.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina
Autor:Cura, C.I.; Lucero, R.H.; Bisio, M.; Oshiro, E.; Formichelli, L.B.; Burgos, J.M.; Lejona, S.; Brusés, B.L.; Hernández, D.O.; Severini, G.V.; Velazquez, E.; Duffy, T.; Anchart, E.; Lattes, R.; Altcheh, J.; Freilij, H.; Diez, M.; Nagel, C.; Vigliano, C.; Favaloro, L.; Favaloro, R.R.; Merino, D.E.; Sosa-Estani, S.; Schijman, A.G.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
Instituto de Medicina Regional, Universidad Nacional Del Nordeste, Av. Las Heras 727, 3500, Resistencia, Chaco, Argentina
Centro Nacional de Diagnóstico e Investigación de Endemo-epidemias (CeNDIE), ANLIS Malbrán, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina
Departamento de Microbiología, Inmunología y Parasitología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Ciudad de Buenos Aires, Argentina
Área de Biología Molecular, Centro de Especialidades Médicas Ambulatorias (CEMAR), San Luis 2020, 2000, Rosario, Santa Fe, Argentina
Consultorio de Chagas, Facultad de Medicina, Universidad Nacional Del Nordeste, Moreno 1240, 3400, Corrientes, Argentina
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina
Instituto de Nefrología, Cabello 3889, 1425, Ciudad de Buenos Aires, Argentina
Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Gallo 1330, 1425, Buenos Aires, Argentina
Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
Palabras clave:Chagas disease; Discrete Typing Unit; PCR; Trypanosoma cruzi; adolescent; adult; aged; Argentina; article; bloodstream infection; Chagas disease; child; chronicity; endemic disease; geographic distribution; graft recipient; human; human tissue; immunocompromised patient; major clinical study; pathogenesis; polymerase chain reaction; priority journal; skin biopsy; taxonomy; tissue distribution; Trypanosoma cruzi; Adolescent; Adult; Aged; Aged, 80 and over; Argentina; Chagas Cardiomyopathy; Chagas Disease; Child; Child, Preschool; DNA, Protozoan; Endemic Diseases; Female; Genetic Variation; Genotype; Heart; Humans; Infant; Male; Middle Aged; Phylogeny; Polymerase Chain Reaction; Trypanosoma cruzi; Young Adult; Trypanosoma cruzi
Año:2012
Volumen:139
Número:4
Página de inicio:516
Página de fin:521
DOI: http://dx.doi.org/10.1017/S0031182011002186
Título revista:Parasitology
Título revista abreviado:Parasitology
ISSN:00311820
CODEN:PARAA
CAS:DNA, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v139_n4_p516_Cura

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Citas:

---------- APA ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., Lejona, S.,..., Schijman, A.G. (2012) . Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina. Parasitology, 139(4), 516-521.
http://dx.doi.org/10.1017/S0031182011002186
---------- CHICAGO ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., et al. "Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina" . Parasitology 139, no. 4 (2012) : 516-521.
http://dx.doi.org/10.1017/S0031182011002186
---------- MLA ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., et al. "Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina" . Parasitology, vol. 139, no. 4, 2012, pp. 516-521.
http://dx.doi.org/10.1017/S0031182011002186
---------- VANCOUVER ----------
Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., et al. Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina. Parasitology. 2012;139(4):516-521.
http://dx.doi.org/10.1017/S0031182011002186