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Abstract:

We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle. © Copyright © Cambridge University Press 2012.

Registro:

Documento: Artículo
Título:Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco
Autor:Maffey, L.; Cardinal, M.V.; Ordóñez-Krasnowski, P.C.; Lanati, L.A.; Lauricella, M.A.; Schijman, A.G.; Gürtler, R.E.
Filiación:Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Instituto Nacional de Parasitología Dr. Mario Fatala Chabén-ANLIS, Buenos Aires, Argentina
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Vuelta de Obligado 2490, Buenos Aires, Argentina
Palabras clave:Chagas disease; Discrete Typing Units; PCR; Triatoma infestans; Triatoma sordida; Trypanosoma cruzi; protozoal DNA; ampoule; Argentina; article; Chagas disease; controlled study; field study; human; nonhuman; opossum; parasite identification; polymerase chain reaction; priority journal; rectum; rural area; Triatoma; Triatoma infestans; Triatoma sordida; Trypanosoma cruzi; Animals; Animals, Domestic; Argentina; Dogs; Enzyme-Linked Immunosorbent Assay; Genotype; Humans; Insect Vectors; Polymerase Chain Reaction; Triatoma; Trypanosoma cruzi
Año:2012
Volumen:139
Número:12
Página de inicio:1570
Página de fin:1579
DOI: http://dx.doi.org/10.1017/S0031182012000856
Título revista:Parasitology
Título revista abreviado:Parasitology
ISSN:00311820
CODEN:PARAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v139_n12_p1570_Maffey

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Citas:

---------- APA ----------
Maffey, L., Cardinal, M.V., Ordóñez-Krasnowski, P.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G. & Gürtler, R.E. (2012) . Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco. Parasitology, 139(12), 1570-1579.
http://dx.doi.org/10.1017/S0031182012000856
---------- CHICAGO ----------
Maffey, L., Cardinal, M.V., Ordóñez-Krasnowski, P.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., et al. "Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco" . Parasitology 139, no. 12 (2012) : 1570-1579.
http://dx.doi.org/10.1017/S0031182012000856
---------- MLA ----------
Maffey, L., Cardinal, M.V., Ordóñez-Krasnowski, P.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., et al. "Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco" . Parasitology, vol. 139, no. 12, 2012, pp. 1570-1579.
http://dx.doi.org/10.1017/S0031182012000856
---------- VANCOUVER ----------
Maffey, L., Cardinal, M.V., Ordóñez-Krasnowski, P.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., et al. Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco. Parasitology. 2012;139(12):1570-1579.
http://dx.doi.org/10.1017/S0031182012000856