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Abstract:

A nutritional characteristic of trypanosomatid protozoa is that in vitro they need a haem-compound as a growth factor, which is supplied as haemoglobin, haematin or haemin. Because haemin and related porphyrins are an important source of oxidative stress in biological systems, the effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi was evaluated. We have observed that, in epimastigotes grown under different haemin concentrations in the culture medium (0-30 mg/l), 5 mg/l was the haemin concentration yielding optimum growth. Above 15 mg/l there was a clear decrease in growth rate, producing the epimastigote to amastigote transformation. Such morphological change was observed together with a marked injury of the enzymatic machinery of the parasite, leading to diminished protein synthesis as well as lower activity of the antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and trypanothione reductase), reduced total thiol content and a marked increase in the HaemOx-1 activity and expression. The current work demonstrates that there is a correlation between higher haemin concentrations in the culture medium and oxidative damage in the cells. Under these conditions induction of HaemOx-1 would indicate the important role of this enzyme as an antioxidant defence response in Trypanosoma cruzi. © 2007 Cambridge University Press.

Registro:

Documento: Artículo
Título:Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
Autor:Ciccarelli, A.; Araujo, L.; Batlle, A.; Lombardo, E.
Filiación:Centro de Investigaciones Sobre Porfirinas Y Porfirias, CONICET-UBA, Argentina
Departamento de Química Biológica, FCEN-UBA, Argentina
Viamonte 1881, 10 A, CP-1056 Buenos Aires, Argentina
Palabras clave:Antioxidant enzymes; Cell transformation; Haeme oxygenase; Haemin; Trypanosoma cruzi; antioxidant; ascorbate peroxidase; heme oxygenase 1; hemin; protozoal protein; superoxide dismutase; thiol; trypanothione reductase; amastigote; article; concentration (parameters); controlled study; culture medium; epimastigote; growth rate; morphology; nonhuman; parasite cultivation; parasite development; priority journal; protein content; protein function; protein synthesis; Trypanosoma cruzi; Animals; Culture Media; Gene Expression; Heme Oxygenase-1; Hemin; NADH, NADPH Oxidoreductases; Oxidative Stress; Peroxidases; Proteins; Sulfhydryl Compounds; Superoxide Dismutase; Time Factors; Trypanosoma cruzi; Protozoa; Trypanosoma cruzi; Trypanosomatidae
Año:2007
Volumen:134
Número:7
Página de inicio:959
Página de fin:965
DOI: http://dx.doi.org/10.1017/S0031182007002399
Título revista:Parasitology
Título revista abreviado:Parasitology
ISSN:00311820
CODEN:PARAA
CAS:ascorbate peroxidase, 72906-87-7; hemin, 16009-13-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; trypanothione reductase, 102210-35-5; ascorbate peroxidase, EC 1.11.1.11; Culture Media; Heme Oxygenase-1, EC 1.14.99.3; Hemin, 16009-13-5; NADH, NADPH Oxidoreductases, EC 1.6.-; Peroxidases, EC 1.11.1.-; Proteins; Sulfhydryl Compounds; Superoxide Dismutase, EC 1.15.1.1; trypanothione reductase, EC 1.8.1.12
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v134_n7_p959_Ciccarelli

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Citas:

---------- APA ----------
Ciccarelli, A., Araujo, L., Batlle, A. & Lombardo, E. (2007) . Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi. Parasitology, 134(7), 959-965.
http://dx.doi.org/10.1017/S0031182007002399
---------- CHICAGO ----------
Ciccarelli, A., Araujo, L., Batlle, A., Lombardo, E. "Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi" . Parasitology 134, no. 7 (2007) : 959-965.
http://dx.doi.org/10.1017/S0031182007002399
---------- MLA ----------
Ciccarelli, A., Araujo, L., Batlle, A., Lombardo, E. "Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi" . Parasitology, vol. 134, no. 7, 2007, pp. 959-965.
http://dx.doi.org/10.1017/S0031182007002399
---------- VANCOUVER ----------
Ciccarelli, A., Araujo, L., Batlle, A., Lombardo, E. Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi. Parasitology. 2007;134(7):959-965.
http://dx.doi.org/10.1017/S0031182007002399