Artículo

Marcet, P.L.; Duffy, T.; Cardinal, M.V.; Burgos, J.M.; Lauricella, M.A.; Levin, M.J.; Kitron, U.; Gürtler, R.E.; Schijman, A.G. "PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina" (2006) Parasitology. 132(1):57-65
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Abstract:

This study applied improved DNA extraction and polymerase chain reaction strategies for screening and identification of Trypanosoma cruzi lineages directly from faeces of triatomines collected in a well-defined rural area in northwestern Argentina. Amplification of the variable regions of the kinetoplastid minicircle genome (kDNA-PCR) was performed in faecal lysates from 33 microscope (MO)-positive and 93 MO-negative Triatoma infestans, 2 MO-positive and 38 MO-negative Triatoma guasayana and 2 MO-positive and 73 MO-negative Triatoma garciabesi. kDNA-PCR detected T. cruzi in 91% MO-positive and 7.5% MO-negative T. infestans, which were confirmed by amplification of the minicircle conserved region. In contrast, kDNA-PCR was negative in all faecal samples from the other triatomine species. A panel of PCR-based genomic markers (intergenic region of spliced-leader DNA, 24Sα and 18S rRNA genes and A10 sequence) was implemented to identify the parasite lineages directly in DNA lysates from faeces and culture isolates from 28 infected specimens. Two were found to be infected with TCI, 24 with TCIIe, 1 with TCIId and 1 revealed a mixed TCI+ TCII infection in the faecal sample whose corresponding culture only showed TCII, providing evidence of the advantages of direct typing of biological samples. This study provides an upgrade in the current diagnosis and lineage identification of T. cruzi in field-collected triatomines and shows T. cruzi II strains as predominant in the region. © 2005 Cambridge University Press.

Registro:

Documento: Artículo
Título:PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina
Autor:Marcet, P.L.; Duffy, T.; Cardinal, M.V.; Burgos, J.M.; Lauricella, M.A.; Levin, M.J.; Kitron, U.; Gürtler, R.E.; Schijman, A.G.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética Y Biología Molecular (INGEBI-CONICET), Vuelta Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética Y Evolución, Ciudad Universitaria, Pab 2, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
Instituto Nacional de Parasitología Dr Mario Fatala Chabén, Buenos Aires, Argentina
College of Veterinary Medicine, University of Illinois, Urbana-Champaign, IL, United States
Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:Chagas disease; Lineage; PCR; Triatoma garciabesi; Triatoma guasayana; Triatoma infestans; Trypanosoma cruzi; Argentina; article; cell culture; cell isolation; cell lineage; Chagas disease; feces analysis; genome; human; marker gene; nonhuman; parasite; parasite identification; polymerase chain reaction; priority journal; protozoal infection; sample; Triatoma; Trypanosoma cruzi; Kinetoplastida; Triatoma; Triatoma garciabesi; Triatoma guasayana; Triatoma infestans; Trypanosoma cruzi
Año:2006
Volumen:132
Número:1
Página de inicio:57
Página de fin:65
DOI: http://dx.doi.org/10.1017/S0031182005008772
Título revista:Parasitology
Título revista abreviado:Parasitology
ISSN:00311820
CODEN:PARAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v132_n1_p57_Marcet

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Citas:

---------- APA ----------
Marcet, P.L., Duffy, T., Cardinal, M.V., Burgos, J.M., Lauricella, M.A., Levin, M.J., Kitron, U.,..., Schijman, A.G. (2006) . PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina. Parasitology, 132(1), 57-65.
http://dx.doi.org/10.1017/S0031182005008772
---------- CHICAGO ----------
Marcet, P.L., Duffy, T., Cardinal, M.V., Burgos, J.M., Lauricella, M.A., Levin, M.J., et al. "PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina" . Parasitology 132, no. 1 (2006) : 57-65.
http://dx.doi.org/10.1017/S0031182005008772
---------- MLA ----------
Marcet, P.L., Duffy, T., Cardinal, M.V., Burgos, J.M., Lauricella, M.A., Levin, M.J., et al. "PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina" . Parasitology, vol. 132, no. 1, 2006, pp. 57-65.
http://dx.doi.org/10.1017/S0031182005008772
---------- VANCOUVER ----------
Marcet, P.L., Duffy, T., Cardinal, M.V., Burgos, J.M., Lauricella, M.A., Levin, M.J., et al. PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina. Parasitology. 2006;132(1):57-65.
http://dx.doi.org/10.1017/S0031182005008772