Artículo
Araya, J.; Cano, M.I.; Gomes, H.B.M.; Novak, E.M.; Requena, J.M.; Alonso, C.; Levin, M.J.; Guevara, P.; Ramirez, J.L.; Franco Da Silveira, J. "Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi" (1997) Parasitology. 115(6):563-570
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Abstract:
We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi. strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome.
Registro:
| Documento: | Artículo |
| Título: | Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
| Autor: | Araya, J.; Cano, M.I.; Gomes, H.B.M.; Novak, E.M.; Requena, J.M.; Alonso, C.; Levin, M.J.; Guevara, P.; Ramirez, J.L.; Franco Da Silveira, J. |
| Filiación: | Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862, CEP 04023-062, SP, Brazil Unidad de Parasitologia, Universidad de Antofagasta, Av. Coloso s/n, Casilla 170, Antofagasta, Chile Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Canto Blanco 28049, Madrid, Spain Ingebi, Obligado 2490, 1428 Buenos Aires, Argentina Centro de Biologia Celular, UCV, Apdo 47525, Caracas, Venezuela |
| Palabras clave: | Diagnostic tool; Genomic distribution; Polymorphic marker; Sequence analysis; Trypanosoma cruzi repetitive DNA; article; dna sequence; gene location; nonhuman; nucleotide repeat; parasite identification; priority journal; sequence analysis; sequence homology; species difference; trypanosoma cruzi; Animals; Base Sequence; Blotting, Southern; Chagas Disease; DNA, Protozoan; Electrophoresis, Gel, Pulsed-Field; Genome, Protozoan; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Genetic; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA; Species Specificity; Trypanosoma cruzi; Protozoa; Trypanosoma; Trypanosoma cruzi |
| Año: | 1997 |
| Volumen: | 115 |
| Número: | 6 |
| Página de inicio: | 563 |
| Página de fin: | 570 |
| DOI: | http://dx.doi.org/10.1017/S0031182097001674 |
| Título revista: | Parasitology |
| Título revista abreviado: | Parasitology |
| ISSN: | 00311820 |
| CODEN: | PARAA |
| CAS: | DNA, Protozoan |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v115_n6_p563_Araya |
Referencias:
- Aksoy, S., Site-specific retrotransposons of the Trypanosomatid protozoa (1991) Parasitology Today, 7, pp. 281-285
- Arruda, M.V., Reinach, F.C., Colli, W., Zingales, B., Sequence of the 24S α ribosomal RNA gene and characterization of the corresponding pseudogene from Trypanosoma cruzi (1990) Molecular and Biochemical Parasitology, 40, pp. 35-42
- Cano, M.I., Gruber, A., Vasquez, M., Cortes, A., Levin, M.J., Gonzalez, A., Degrave, W., Franco Da Silveira, J., Trypanosoma cruzi genome project: Molecular karyotype of clone CL Brener (1995) Molecular and Biochemical Parasitology, 71, pp. 273-278
- Castro, C., Craig, S.P., Castaneda, M., Genomic organization and ploidy number in T. cruzi (1981) Molecular and Biochemical Parasitology, 4, pp. 273-282
- Cotrim, P.C., Cummings, L.M., Franco Da Silveira, J., Characterization of Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence (1989) Memórias do Instituto Oswaldo Cruz, 84, pp. 143-149
- D'Alessandro, A., Biology of Trypanosoma cruzi (Hepertosoma) rangeli Tejera 1920 (1976) Biology of Kinetoplastida, 1, pp. 328-393. , ed. Lumdsen, W. M. R. & Evans, D. A., Academic Press, New York
- Devereux, J., Haerberli, P., Smithies, O., A comprehensive set of sequence analysis programs for the VAX (1984) Nucleic Acids Research, 12, pp. 387-395
- Frasch, A.C.C., Carrasco, A.E., Goijman, S.G., Sanchez, D.O., Repetitive sequences scattered throughout the genome of Trypanosoma cruzi (1983) Molecular and Biochemical Parasitology, 8, pp. 227-239
- Gonzalez, A., Prediger, E., Huecas, M.E., Nogueira, N., Lizardi, P.Ḿ., Minichromosomal repetitive DNA in Trypanosoma cruzi: Its use in a high-sensitivity parasite detection assay (1984) Proceedings of the National Academy of Sciences, USA, 81, pp. 3356-3360
- Gonzalez, N., Galindo, I., Guevara, P., Novak, E., Scorza, J.V., Anez, N., Franco Da Silveira, J., Ramirez, J.L., Identification of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer (1994) Journal of Clinical Microbiology, 32, pp. 153-158
- Hasan, G., Turner, M.J., Cordingley, J.S., Complete nucleotide sequence of an unusual mobile element from Trypanosoma brucei (1984) Cell, 37, pp. 333-341
- Kimmel, B.E., Onesmo, K., Ole-Mioyoi, O.K., Young, J.R., Ingi, a 5·2 kb dispersed element from Trypanosoma brucei that carries half a smaller mobile element at either end and has homology with mammalian LINES (1987) Molecular Cell Biology, 7, pp. 1465-1475
- Lanar, D.E., Levy, S.C., Manning, J.E., Complexity and content of the DNA and RNA in Trypanosoma cruzi (1981) Molecular and Biochemical Parasitology, 3, pp. 327-341
Citas:
---------- APA ----------
Araya, J., Cano, M.I., Gomes, H.B.M., Novak, E.M., Requena, J.M., Alonso, C., Levin, M.J.,..., Franco Da Silveira, J. (1997) . Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi. Parasitology, 115(6), 563-570.http://dx.doi.org/10.1017/S0031182097001674
---------- CHICAGO ----------
Araya, J., Cano, M.I., Gomes, H.B.M., Novak, E.M., Requena, J.M., Alonso, C., et al. "Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi" . Parasitology 115, no. 6 (1997) : 563-570.http://dx.doi.org/10.1017/S0031182097001674
---------- MLA ----------
Araya, J., Cano, M.I., Gomes, H.B.M., Novak, E.M., Requena, J.M., Alonso, C., et al. "Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi" . Parasitology, vol. 115, no. 6, 1997, pp. 563-570.http://dx.doi.org/10.1017/S0031182097001674
---------- VANCOUVER ----------
Araya, J., Cano, M.I., Gomes, H.B.M., Novak, E.M., Requena, J.M., Alonso, C., et al. Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi. Parasitology. 1997;115(6):563-570.http://dx.doi.org/10.1017/S0031182097001674
