Abstract:
A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors. © 1997 Elsevier Science B.V.
Registro:
Documento: |
Artículo
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Título: | Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics |
Autor: | Torga, J.R.; Etcheverry, J.I.; Marconi, M.C. |
Filiación: | Departamento de Fisica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 1, 1428 Buenos Aires, Argentina Departamento de Matemática, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 1, 1428 Buenos Aires, Argentina
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Palabras clave: | Brownian dynamics; Fluorescence anisotropy; Rotational depolarization; Brownian movement; Fluorescence; Molecular dynamics; Rotational depolarization; Laser pulses |
Año: | 1997
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Volumen: | 143
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Número: | 4-6
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Página de inicio: | 230
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Página de fin: | 234
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DOI: |
http://dx.doi.org/10.1016/S0030-4018(97)00314-3 |
Título revista: | Optics Communications
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Título revista abreviado: | Opt Commun
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ISSN: | 00304018
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CODEN: | OPCOB
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00304018_v143_n4-6_p230_Torga |
Referencias:
- Lakowicz, J.R., (1986) Principles of Fluorescence Spectroscopy, , Plenum, New York
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- Perrin, F., (1936) Acta Phys. Pol., 5, p. 335
- Jablonski, A., (1960) Bull. Acad. Pol. Sci., 8, p. 259
- Weber, G., (1989) J. Phys. Chem., 93, p. 6069
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- Kawski, A., (1993) Crit. Rev. Anal. Chem., 23, p. 459
- Spaans, E.A.J., Van Exter, M.P., Lagendijk, A., (1990) Optics Comm., 76, p. 376
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Citas:
---------- APA ----------
Torga, J.R., Etcheverry, J.I. & Marconi, M.C.
(1997)
. Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics. Optics Communications, 143(4-6), 230-234.
http://dx.doi.org/10.1016/S0030-4018(97)00314-3---------- CHICAGO ----------
Torga, J.R., Etcheverry, J.I., Marconi, M.C.
"Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics"
. Optics Communications 143, no. 4-6
(1997) : 230-234.
http://dx.doi.org/10.1016/S0030-4018(97)00314-3---------- MLA ----------
Torga, J.R., Etcheverry, J.I., Marconi, M.C.
"Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics"
. Optics Communications, vol. 143, no. 4-6, 1997, pp. 230-234.
http://dx.doi.org/10.1016/S0030-4018(97)00314-3---------- VANCOUVER ----------
Torga, J.R., Etcheverry, J.I., Marconi, M.C. Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics. Opt Commun. 1997;143(4-6):230-234.
http://dx.doi.org/10.1016/S0030-4018(97)00314-3