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Abstract:

MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).

Registro:

Documento: Artículo
Título:Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
Autor:Gudesblat, G.E.; Iusem, N.D.; Morris, P.C.
Filiación:Laboratorio de Fisiología, Biología Molecular y Celular, IFIByNE - CONICET, Facultad de Ciencias Exactas y Naturales - UBA, 1428 Buenos Aires, Argentina
Instituto Leloir, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina
School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, United Kingdom
Palabras clave:Abscisic acid (ABA); Arabidopsis thaliana; Hydrogen peroxide (H 2O2); MAP kinase; MPK3; Stomata; abscisic acid; Arabidopsis protein; complementary DNA; hydrogen peroxide; mitogen activated protein kinase 3; abnormality; abscisic acid; alkalinization; enzyme activity; gene expression; hydrogen peroxide; inhibition; RNA; signal; stomatal conductance; transgenic plant; Arabidopsis; article; cytology; genetic transformation; genetics; metabolism; physiology; plant epidermis; plant leaf; signal transduction; transgenic plant; Abscisic Acid; Arabidopsis; Arabidopsis Proteins; DNA, Antisense; Hydrogen Peroxide; Mitogen-Activated Protein Kinase 3; Plant Epidermis; Plant Leaves; Plants, Genetically Modified; Signal Transduction; Transformation, Genetic; Arabidopsis; Arabidopsis thaliana
Año:2007
Volumen:173
Número:4
Página de inicio:713
Página de fin:721
DOI: http://dx.doi.org/10.1111/j.1469-8137.2006.01953.x
Handle:http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat
Título revista:New Phytologist
Título revista abreviado:New Phytol.
ISSN:0028646X
CODEN:NEPHA
CAS:abscisic acid, 21293-29-8; hydrogen peroxide, 7722-84-1; mitogen activated protein kinase 3, 137632-07-6; Abscisic Acid, 21293-29-8; Arabidopsis Proteins; DNA, Antisense; Hydrogen Peroxide, 7722-84-1; Mitogen-Activated Protein Kinase 3, EC 2.7.1.37
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0028646X_v173_n4_p713_Gudesblat.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0028646X_v173_n4_p713_Gudesblat

Referencias:

  • Ahlfors, R., Macioszek, V., Rudd, J., Brosche, M., Schlichting, R., Scheel, D., Kangasjarvi, J., Stress hormone-independent activation and nuclear translocation of mitogen-activated protein kinases in Arabidopsis thaliana during ozone exposure (2004) Plant Journal, 40, pp. 512-522
  • Apel, K., Hirt, H., Reactive oxygen species: Metabolism, oxidative stress, and signal transduction (2004) Annual Review of Plant Biology, 55, pp. 373-399
  • Asai, T., Tena, G., Plotnikova, J., Willmann, M.R., Chiu, W.L., Gomez-Gomez, L., Boller, T., Sheen, J., MAP kinase signalling cascade in Arabidopsis innate immunity (2002) Nature, 415, pp. 977-983
  • Blatt, M.R., Armstrong, F., K+ channels of stomatal guard cells: Abscisic acid evoked control of the outward rectifier mediated by cytoplasmic pH (1993) Planta, 191, pp. 330-341
  • Burnett, E.C., Desikan, R., Moser, R.C., Neill, S.J., ABA activation of an MBP kinase in Pisum sativum epidermal peels correlates with stomatal responses to ABA (2000) Journal of Experimental Botany, 51, pp. 197-205
  • Chen, Y.L., Huang, R.F., Xiao, Y.M., Lu, P., Chen, J., Wang, X.C., Extracellular calmodulin-induced stomatal closure is mediated by heterotrimeric G protein and H2O2 (2004) Plant Physiology, 136, pp. 4096-4103
  • Clough, S.J., Bent, A.F., Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana (1998) Plant Journal, 16, pp. 735-743
  • Desikan, R., Cheung, M.K., Bright, J., Henson, D., Hancock, J.T., Neill, S.J., ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells (2004) Journal of Experimental Botany, 55, pp. 205-212
  • Desikan, R., Cheung, M.K., Clarke, A., Golding, S., Sagi, M., Fluhr, R., Rock, C., Neill, S., Hydrogen peroxide is a common signal for darkness- and ABA-induced stomatal closure in Pisum sativum (2004) Functional Plant Biology, 31, pp. 913-920
  • Droillard, M.J., Thibivilliers, S., Cazale, A.C., Barbier-Brygoo, H., Lauriere, C., Protein kinases induced by osmotic stresses and elicitor molecules in tobacco cell suspensions: Two crossroad MAP kinases and one osmoregulation-specific protein kinase (2000) FEBS Letters, 474, pp. 217-222
  • Fan, L.M., Zhao, Z.X., Assmann, S.M., Guard cells: A dynamic signaling model (2004) Current Opinion in Plant Biology, 7, pp. 537-546
  • Foyer, C.H., Noctor, G., Redox homeostasis and antioxidant signaling: A metabolic interface between stress perception and physiological responses (2005) Plant Cell, 17, pp. 1866-1875
  • Guan, L.M., Zhao, J., Scandalios, J.G., Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O 2 is the likely intermediary signaling molecule for the response (2000) Plant Journal, 22, pp. 87-95
  • Hetherington, A.M., Woodward, F.I., The role of stomata in sensing and driving environmental change (2003) Nature, 424, pp. 901-908
  • Hunt, L., Mills, L.N., Pical, C., Leckie, C.P., Aitken, F.L., Kopka, J., Mueller-Roeber, B., Gray, J.E., Phospholipase C is required for the control of stomatal aperture by ABA (2003) Plant Journal, 34, pp. 47-55
  • Jiang, J., An, G.Y., Wang, P.C., Wang, P.T., Han, J.F., Jin, Y.B., Song, C.P., MAP kinase specifically mediates the ABA-induced H2O2 generation in guard cells of Vicia faba L (2003) Chinese Science Bulletin, 48, pp. 1919-1926
  • Jonak, C., Okresz, L., Bogre, L., Hirt, H., Complexity, cross talk and integration of plant MAP kinase signalling (2002) Current Opinion in Plant Biology, 5, pp. 415-424
  • Joo, J.H., Wang, S.Y., Chen, J.G., Jones, A.M., Fedoroff, N.V., Different signaling and cell death roles of heterotrimeric G protein alpha and beta subunits in the Arabidopsis oxidative stress response to ozone (2005) Plant Cell, 17, pp. 957-970
  • Kovtun, Y., Chiu, W.L., Tena, G., Sheen, J., Functional analysis of oxidative stress-activated mitogen- activated protein kinase cascade in plants (2000) Proceedings of the National Academy of Sciences, USA, 97, pp. 2940-2945
  • Kwak, J.M., Kim, S.A., Hong, S.W., Nam, H.G., Evaluation of 515 expressed sequence tags obtained from guard cells of Brassica campestris (1997) Planta, 202, pp. 9-17
  • Kwak, J.M., Mori, I.C., Pei, Z.M., Leonhardt, N., Torres, M.A., Dangl, J.L., Bloom, R.E., Schroeder, J.I., NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis (2003) EMBO Journal, 22, pp. 2623-2633
  • Lee, S., Choi, H., Suh, S., Doo, I.S., Oh, K.Y., Choi, E.J., Taylor, A.T.S., Lee, Y., Oligogalacturonic acid and chitosan reduce stomatal aperture by inducing the evolution of reactive oxygen species from guard cells of tomato and Commelina communis (1999) Plant Physiology, 121, pp. 147-152
  • Lu, C., Han, M.H., Guevara-Garcia, A., Fedoroff, N.V., Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid (2002) Proceedings of the National Academy of Sciences, USA, 99, pp. 15812-15817
  • McAinsh, M.R., Clayton, H., Mansfield, T.A., Hetherington, A.M., Changes in stomatal behavior and guard cell cytosolic free calcium in response to oxidative stress (1996) Plant Physiology, 111, pp. 1031-1042
  • Mishra, G., Zhang, W., Deng, F., Zhao, J., Wang, X., A bifurcating pathway directs abscisic acid effects on stomatal closure and opening in Arabidopsis (2006) Science, 312, pp. 264-266
  • Mittler, R., Vanderauwera, S., Gollery, M., Van Breusegem, F., Reactive oxygen gene network of plants (2004) Trends in Plant Science, 9, pp. 490-498
  • Mizoguchi, T., Irie, K., Hirayama, T., Hayashida, N., Yamaguchi-Shinozaki, K., Matsumoto, K., Shinozaki, K., A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana (1996) Proceedings of the National Academy of Sciences, USA, 93, pp. 765-769
  • Mori, I.C., Muto, S., Abscisic acid activates a 48-kilodalton protein kinase in guard cell protoplasts (1997) Plant Physiology, 113, pp. 833-839
  • Morris, P.C., MAP kinase signal transduction pathways in plants (2001) New Phytologist, 151, pp. 67-89
  • Murata, Y., Pei, Z.M., Mori, I.C., Schroeder, J., Abscisic acid activation of plasma membrane Ca2+ channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants (2001) Plant Cell, 13, pp. 2513-2523
  • Mustilli, A.C., Merlot, S., Vavasseur, A., Fenzi, F., Giraudat, J., Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production (2002) Plant Cell, 14, pp. 3089-3099
  • Nakagami, H., Pitzschke, A., Hirt, H., Emerging MAP kinase pathways in plant stress signalling (2005) Trends in Plant Science, 10, pp. 339-346
  • Newman, T., de Bruijn, F.J., Green, P., Keegstra, K., Kende, H., McIntosh, L., Ohlrogge, J., Thomashow, M.A., Genes galore: A summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones (1994) Plant Physiology, 106, pp. 1241-1255
  • Pei, Z.M., Murata, Y., Benning, G., Thomine, S., Klusener, B., Allen, G.J., Grill, E., Schroeder, J.I., Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells (2000) Nature, 406, pp. 731-734
  • Petersen, M., Brodersen, P., Naested, H., Andreasson, E., Lindhart, U., Johansen, B., Nielsen, H.B., Mundy, J., Arabidopsis MAP kinase 4 negatively regulates systemic acquired resistance (2000) Cell, 103, pp. 1111-1120
  • Pitzschke, A., Hirt, H., Mitogen-activated protein kinases and reactive oxygen species signaling in plants (2006) Plant Physiology, 141, pp. 351-356
  • Plesch, G., Ehrhardt, T., Mueller-Roeber, B., Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cell-specific gene expression (2001) Plant Journal, 28, pp. 455-464
  • Ren, D.T., Yang, H.P., Zhang, S.Q., Cell death mediated by MAPK is associated with hydrogen peroxide production in Arabidopsis (2002) Journal of Biological Chemistry, 277, pp. 559-565
  • Roelfsema, M.R., Hedrich, R., In the light of stomatal opening: New insights into 'the Watergate' (2005) New Phytologist, 167, pp. 665-691
  • Schenk, P.M., Kazan, K., Manners, J.M., Anderson, J.P., Simpson, R.S., Wilson, I.W., Somerville, S.C., Maclean, D.J., Systemic gene expression in Arabidopsis during an incompatible interaction with Alternaria brassicicola (2003) Plant Physiology, 132, pp. 999-1010
  • She, X.P., Song, X.G., He, J.M., Role and relationship of nitric oxide and hydrogen peroxide in light/dark-regulated stomntal movement in Vicia faba (2004) Acta Botanica Sinica, 46, pp. 1292-1300
  • Suhita, D., Raghavendra, A.S., Kwak, J.M., Vavasseur, A., Cytoplasmic alkalization precedes reactive oxygen species production during methyl jasmonate- and abscisic acid-induced stomatal closure (2004) Plant Physiology, 134, pp. 1536-1545
  • Wang, X.Q., Ullah, H., Jones, A.M., Assmann, S.M., G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells (2001) Science, 292, pp. 2070-2072
  • Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G., Doke, N., Nicotiana benthamiana gp91 (phox) homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans (2003) Plant Cell, 5, pp. 706-718
  • Zhang, X., Zhang, L., Dong, F.C., Gao, J.F., Galbraith, D.W., Song, C.P., Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba (2001) Plant Physiology, 126, pp. 1438-1448

Citas:

---------- APA ----------
Gudesblat, G.E., Iusem, N.D. & Morris, P.C. (2007) . Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide. New Phytologist, 173(4), 713-721.
http://dx.doi.org/10.1111/j.1469-8137.2006.01953.x
---------- CHICAGO ----------
Gudesblat, G.E., Iusem, N.D., Morris, P.C. "Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide" . New Phytologist 173, no. 4 (2007) : 713-721.
http://dx.doi.org/10.1111/j.1469-8137.2006.01953.x
---------- MLA ----------
Gudesblat, G.E., Iusem, N.D., Morris, P.C. "Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide" . New Phytologist, vol. 173, no. 4, 2007, pp. 713-721.
http://dx.doi.org/10.1111/j.1469-8137.2006.01953.x
---------- VANCOUVER ----------
Gudesblat, G.E., Iusem, N.D., Morris, P.C. Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide. New Phytol. 2007;173(4):713-721.
http://dx.doi.org/10.1111/j.1469-8137.2006.01953.x