Artículo

Rothlin, C.V.; Katz, E.; Verbitsky, M.; Vetter, D.E.; Heinemann, S.F.; Elgoyhen, A.B. "Block of the α9 nicotinic receptor by ototoxic aminoglycosides" (2000) Neuropharmacology. 39(13):2525-2532
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Abstract:

In the present study, we report that the α9 nicotinic acetylcholine receptor (nAChR) expressed in Xenopus laevis oocytes is reversibly blocked by aminoglycoside antibiotics. The aminoglycosides tested blocked the α9 nAChR in a concentration-dependent manner with the following rank order of potency: neomycin>gentamicin>streptomycin>amikacin>kanamycin. The antagonistic effect of gentamicin was not overcome by increasing the concentration of acetylcholine (ACh), indicative of a non-competitive type of block. Blockage of ACh-evoked currents by gentamicin was found to be voltage-dependent, being more potent at hyperpolarized than at depolarized holding potentials. Furthermore, gentamicin blockage was dependent upon the extracellular Ca2+ concentration, shown by the fact that increments in extracellular Ca2+ significantly reduced the potency of this aminoglycoside to block the α9 nAChR. Possible mechanisms of blockage by the aminoglycosides are discussed. The present results suggest that the initial reversible actions of aminoglycosides at the organ of Corti, such as the elimination of the olivocochlear efferent function, are due in part to the interaction with the native α9-containing cholinergic receptor of the outer hair cells. Copyright (C) 2000 Elsevier Science Ltd.

Registro:

Documento: Artículo
Título:Block of the α9 nicotinic receptor by ototoxic aminoglycosides
Autor:Rothlin, C.V.; Katz, E.; Verbitsky, M.; Vetter, D.E.; Heinemann, S.F.; Elgoyhen, A.B.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92037, United States
Palabras clave:Aminoglycosides; Hair cells; Nicotinic receptors; Ototoxicity; acetylcholine; amikacin; aminoglycoside; aminoglycoside antibiotic agent; gentamicin; kanamycin; neomycin; nicotinic receptor; streptomycin; 1,2 bis(2 aminophenoxy)ethane N,N,N',N' tetraacetic acid acetoxymethyl ester; 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; antiinfective agent; calcium; chelating agent; Chrna9 protein, rat; drug derivative; egtazic acid; gentamicin; nicotinic receptor; nicotinic receptor blocking agent; animal cell; article; binding site; controlled study; Corti organ; drug potency; drug receptor binding; hair cell; nonhuman; oocyte; ototoxicity; priority journal; receptor blocking; Xenopus laevis; animal; chemically induced disorder; drug effect; electrophysiology; electrostimulation; hearing disorder; metabolism; olivary nucleus; rat; Xenopus; Animals; Anti-Bacterial Agents; Calcium; Chelating Agents; Egtazic Acid; Electric Stimulation; Electrophysiology; Gentamicins; Hearing Disorders; Nicotinic Antagonists; Olivary Nucleus; Oocytes; Rats; Receptors, Nicotinic; Xenopus
Año:2000
Volumen:39
Número:13
Página de inicio:2525
Página de fin:2532
DOI: http://dx.doi.org/10.1016/S0028-3908(00)00056-3
Título revista:Neuropharmacology
Título revista abreviado:Neuropharmacology
ISSN:00283908
CODEN:NEPHB
CAS:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester, 139890-68-9; Anti-Bacterial Agents; Calcium, 7440-70-2; Chelating Agents; Chrna9 protein, rat; Egtazic Acid, 67-42-5; Gentamicins; Nicotinic Antagonists; Receptors, Nicotinic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283908_v39_n13_p2525_Rothlin

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Citas:

---------- APA ----------
Rothlin, C.V., Katz, E., Verbitsky, M., Vetter, D.E., Heinemann, S.F. & Elgoyhen, A.B. (2000) . Block of the α9 nicotinic receptor by ototoxic aminoglycosides. Neuropharmacology, 39(13), 2525-2532.
http://dx.doi.org/10.1016/S0028-3908(00)00056-3
---------- CHICAGO ----------
Rothlin, C.V., Katz, E., Verbitsky, M., Vetter, D.E., Heinemann, S.F., Elgoyhen, A.B. "Block of the α9 nicotinic receptor by ototoxic aminoglycosides" . Neuropharmacology 39, no. 13 (2000) : 2525-2532.
http://dx.doi.org/10.1016/S0028-3908(00)00056-3
---------- MLA ----------
Rothlin, C.V., Katz, E., Verbitsky, M., Vetter, D.E., Heinemann, S.F., Elgoyhen, A.B. "Block of the α9 nicotinic receptor by ototoxic aminoglycosides" . Neuropharmacology, vol. 39, no. 13, 2000, pp. 2525-2532.
http://dx.doi.org/10.1016/S0028-3908(00)00056-3
---------- VANCOUVER ----------
Rothlin, C.V., Katz, E., Verbitsky, M., Vetter, D.E., Heinemann, S.F., Elgoyhen, A.B. Block of the α9 nicotinic receptor by ototoxic aminoglycosides. Neuropharmacology. 2000;39(13):2525-2532.
http://dx.doi.org/10.1016/S0028-3908(00)00056-3