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Abstract:

The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis. Copyright © 2010 S. Karger AG.

Registro:

Documento: Artículo
Título:Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
Autor:Schaaf, C.; Shan, B.; Onofri, C.; Stalla, G.K.; Arzt, E.; Schilling, T.; Perone, M.J.; Renner, U.
Filiación:Max Planck Institute of Psychiatry, Neuroendocrinology Group, Munich, Germany
Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología y Biología Molecular y Celular, Universidad de Buenos Aires and IFIBYNE-CONICET, Buenos Aires, Argentina
Department of Internal Medicine, Endocrinology, Metabolism and Clinical Chemistry, University of Heidelberg, Heidelberg, Germany
Palabras clave:Apoptosis; Cell cycle; Curcumin; Folliculostellate pituitary cells; Interleukin-6; Nuclear factor-κB; Proliferation; Tlr4; Vascular endothelial growth factor; 7 aminodactinomycin; agar; caspase 3; curcumin; cyclin D1; DNA; endotoxin; fluorescein isothiocyanate; immunoglobulin enhancer binding protein; interleukin 6; lipocortin 5; messenger RNA; protein bcl 2; toll like receptor 4; vasculotropin A; angiogenesis; animal cell; antineoplastic activity; apoptosis; article; cell culture; cell cycle arrest; cell cycle G2 phase; cell cycle M phase; cell death; cell growth; cell proliferation; cell type; colony formation; controlled study; cytokine production; cytokine release; DNA fragmentation; drug inhibition; endocrine cell; fluorescence activated cell sorting; growth inhibition; hypophysis cell; immune response; immunohistochemistry; monolayer culture; nonhuman; nucleotide sequence; pituitary folliculostellate cell; priority journal; protein cleavage; protein expression; receptor down regulation; RNA synthesis; Animals; Anti-Inflammatory Agents, Non-Steroidal; Apoptosis; Cell Cycle; Cell Division; Cell Line, Tumor; Curcumin; Interleukin-6; Male; Mice; Pituitary Gland, Anterior; Pituitary Neoplasms; Rats; Rats, Sprague-Dawley; RNA, Messenger; Vascular Endothelial Growth Factor A
Año:2010
Volumen:91
Número:2
Página de inicio:200
Página de fin:210
DOI: http://dx.doi.org/10.1159/000287236
Título revista:Neuroendocrinology
Título revista abreviado:Neuroendocrinology
ISSN:00283835
CODEN:NUNDA
CAS:7 aminodactinomycin, 7240-37-1; DNA, 9007-49-2; agar, 9002-18-0; caspase 3, 169592-56-7; curcumin, 458-37-7; fluorescein isothiocyanate, 25168-13-2, 27072-45-3, 3326-32-7; lipocortin 5, 111237-10-6; protein bcl 2, 219306-68-0; toll like receptor 4, 203811-83-0; vasculotropin A, 489395-96-2; Anti-Inflammatory Agents, Non-Steroidal; Curcumin, 458-37-7; Interleukin-6; RNA, Messenger; Vascular Endothelial Growth Factor A; vascular endothelial growth factor A, mouse; vascular endothelial growth factor A, rat
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v91_n2_p200_Schaaf

Referencias:

  • Rinehart, J.F., Farquhar, M.G., Electron microscopic studies of the anterior pituitary gland (1953) J Histochem Cytochem, 1, pp. 93-113
  • Inoue, K., Couch, E.F., Takano, K., Ogawa, S., The structure and function of folliculo-stellate cells in the anterior pituitary gland (1999) Arch Histol Cytol, 62, pp. 205-218
  • Allaerts, W., Vankelecom, H., History and perspectives of pituitary folliculo-stellate cell research (2005) Eur J Endocrinol, 153, pp. 1-12
  • Fauquier, T., Guerineau, N.C., McKinney, R.A., Bauer, K., Mollard, P., Folliculostellate cell network: A route for long-distance communication in the anterior pituitary (2001) Proc Natl Acad Sci USA, 98, pp. 8891-8896
  • Fauquier, T., Lacampagne, A., Travo, P., Bauer, K., Mollard, P., Hidden face of the anterior pituitary (2002) Trends Endocrinol Metab, 13, pp. 304-309
  • Morand, I., Fonlupt, P., Guerrier, A., Trouillas, J., Calle, A., Remy, C., Rousset, B., Munari-Silem, Y., Cell-to-cell communication in the anterior pituitary: Evidence for gap junction-mediated exchanges between endocrine cells and folliculostellate cells (1996) Endocrinology, 137, pp. 3356-3367
  • Lewis, B.M., Pexa, A., Francis, K., Verma, V., Mc- Nicol, A.M., Scanlon, M., Deussen, A., Ham, J., Adenosine stimulates connexin 43 expression and gap junctional communication in pituitary folliculostellate cells (2006) FASEB J, 20, pp. 2585-2587
  • Meilleur, M.A., Akpovi, C.D., Pelletier, R.M., Vitale, M.L., Tumor necrosis factor-α -induced anterior pituitary folliculostellate TtT/GF cell uncoupling is mediated by connexin 43 dephosphorylation (2007) Endocrinology, 148, pp. 5913-5924
  • Aoki, A., De Gaisan, E.O., Pasolli, H.A., Torres, A.I., Disposal of cell debris from surplus lactotrophs of pituitary gland (1996) Exp Clin Endocrinol Diabetes, 104, pp. 256-262
  • Luziga, C., Yamamoto, Y., Horii, Y., Mbassa, G., Mamba, K., Phagocytotic removal of apoptotic endocrine cells by folliculostellate cells and its functional implications in clusterin accumulation in pituitary colloids in helmeted guinea fowl (Numida meleagris) (2006) Acta Histochem, 108, pp. 69-80
  • Allaerts, W., Jeucken, P.H.M., Debets, R., Hoefakker, S., Claassen, E., Drexhage, H.A., Heterogeneity of pituitary folliculo-stellate cells: Implications for IL-6 production and accessory function in vitro (1997) J Neuroendocrinol, 9, pp. 43-53
  • Fauquier, T., Rizzoti, K., Dattani, M., Lovell- Bage, R., Robinson, I.C., SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland (2008) Proc Natl Acad Sci USA, 105, pp. 2907-2912
  • Gospodarowicz, D., Abraham, J.A., Schilling, J., Isolation and characterization of a vascular cell mitogen produced by pituitary-derived folliculostellate cells (1989) Proc Natl Acad Sci USA, 6, pp. 7311-7315
  • Vankelecom, H., Carmeliet, P., Van Damme, J., Billiau, A., Denef, C., Production of interleukin-6 by folliculo-stellate cells of the anterior pituitary gland in a histiotypic cell aggregate culture system (1989) Neuroendocrinology, 49, pp. 102-106
  • Renner, U., De Santana, E.C., Gerez, J., Fröhlich, B., Haedo, M., Pereda, M.P., Onofri, C., Arzt, E., Intrapituitary expression and regulation of the gp130 cytokine interleukin-6 and its implication in pituitary physiology and pathophysiology (2009) Ann N y Acad Sci, 1153, pp. 89-97
  • Ferrara, N., Vascular endothelial growth factor: Basic science and clinical progress (2004) Endocr Rev, 25, pp. 581-611
  • Lohrer, P., Gloddek, J., Nagashima, A.C., Korali, Z., Hopfner, U., Pereda, M.P., Arzt, E., Renner, U., Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway (2000) Endocrinology, 141, pp. 4457-4465
  • Gloddek, J., Lohrer, P., Stalla, J., Arzt, E., Stalla, G.K., Renner, U., The intrapituitary stimulatory effect of lipopolysaccharide on ACTH secretion is mediated by paracrine-acting IL-6 (2001) Exp Clin Endocrinol Diabetes, 109, pp. 410-415
  • Aggarwal, B.B., Sundaram, C., Malani, N., Ichikawa, H., Curcumin: The Indian solid gold (2007) Adv Exp Med Biol, 595, pp. 1-75
  • Hatcher, H., Planalp, R., Cho, J., Torti, F.M., Torti, S.V., Curcumin: From ancient medicine to current clinical trials (2008) Cell Mol Life Sci, 65, pp. 1631-1652
  • Shishodia, S., Chaturvedi, M.M., Aggarwal, B.B., Role of curcumin in cancer therapy (2007) Current Probl Cancer, 31, pp. 243-305
  • Singh, S., From exotic spice to modern drug? (2007) Cell, 130, pp. 765-768
  • Sa, G., Das, T., Anti cancer effects of curcumin: Cycle of life and death (2008) Cell Div, 3, pp. 1-14
  • Shankar, S., Ganapathy, S., Chen, Q., Srivastava, R.K., Curcumin sensitizes TRAIL-resistant xenografts: Molecular mechanisms of apoptosis, metastasis and angiogenesis (2008) Mol Cancer, 7, p. 16
  • Corson, T.W., Crews, C.M., Molecular understanding and modern application of traditional medicines: Triumphs and trials (2007) Cell, 130, pp. 769-774
  • Dhillon, N., Aggarwal, B.B., Newman, R.A., Wolff, R.A., Kunnumakkara, A.B., Abbruzzese, J.L., Ng, C.S., Kurzrock, R., Phase II trial of curcumin in patients with advanced pancreatic cancer (2008) Clin Cancer Res, 14, pp. 4491-4499
  • Johnson, J.J., Mukhtar, H., Curcumin for chemoprevention of colon cancer (2007) Cancer Lett, 255, pp. 170-181
  • Kunnumakkara, A.B., Anand, P., Aggarwal, B.B., Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signalling proteins (2008) Cancer Lett, 269, pp. 199-225
  • Carroll, C.E., Ellersieck, M.R., Hyder, S.M., Curcumin inhibits MPA-induced secretion of VEGF from T47-D human breast cancer cells (2008) Menopause, 15, pp. 570-574
  • Chadalapaka, G., Jutooru, I., Chintharlapalli, S., Papineni, S., Smith III, R., Li, X., Safe, S., Curcumin decreases specificity protein expression in bladder cancer cells (2008) Cancer Res, 68, pp. 5345-5354
  • Sandur, S.K., Ichikawa, H., Pandey, M.K., Kunnumakkara, A.B., Sung, B., Sethi, G., Aggarwal, B.B., Role of pro-oxidants and antioxidants in the anti-inflammatory and apoptotic effects of curcumin (diferuloylmethane) (2007) Free Radic Biol Med, 43, pp. 568-580
  • Miller, M., Chen, S., Woodliff, J., Kansra, S., Curcumin (diferuloylmethane) inhibits cell proliferation, induces apoptosis, and decreases hormone levels and secretion in pituitary tumor cells (2008) Endocrinology, 149, pp. 4158-4167
  • Inoue, K., Matsumoto, H., Koyama, C., Shibata, K., Nakazato, Y., Ito, A., Establishment of a folliculostellate- like cell line from a murine thyrotropic pituitary tumor (1992) Endocrinology, 131, pp. 3110-3116
  • Renner, U., Newton, C.J., Pagotto, U., Sauer, J., Arzt, E., Stalla, G.K., Involvement of interleukin-1 and interleukin-1 receptor antagonist in rat pituitary cell growth regulation (1995) Endocrinology, 136, pp. 3186-3193
  • Onofri, C., Theodoropoulou, M., Losa, M., Uhl, E., Lange, M., Arzt, E., Stalla, G.K., Renner, U., Localization of vascular endothelial growth factor (VEGF) receptors in normal and adenomatous pituitaries: Detection of a non-endothelial function of VEGF in pituitary tumors (2006) J Endocrinol, 191, pp. 249-261
  • Nolan, L.A., Kavanagh, E., Lightman, S.L., Levy, A., Anterior pituitary cell population control: Basal turnover and the effects of adrenalectomy and dexamethasone treatment (1998) J Neuroendocrinol, 10, pp. 207-215
  • Levy, A., Physiological implications of pituitary trophic activity (2002) J Endocrinol, 174, pp. 147-155
  • Besedovsky, H.O., Del Rey, A., Immune-neuroendocrine interactions: Facts and hypotheses (1996) Endocr Rev, 17, pp. 64-102
  • Herkenham, M., Folliculo-stellate (FS) cells of the anterior pituitary mediate interactions between the endocrine and immune systems (2005) Endocrinology, 146, pp. 33-34
  • Gautam, S.C., Gao, X., Dulchavsky, S., Immunomodulation by curcumin (2007) Adv Exp Med Biol, 595, pp. 321-341
  • Lubbad, A., Oriowo, M.A., Kahn, I., Curcumin attenuates inflammation through inhibition of TLR-4 receptor in experimental colitis (2009) Mol Cell Biochem, 322, pp. 127-135
  • Harper, S.J., Bates, D.O., VEGF-A splicing: The key to anti-angiogenic therapeutics? (2008) Nat Rev Cancer, 8, pp. 880-887
  • Jabbour, H.N., Boddy, S.C., Lincoln, G.A., Pattern of localisation of expression of vascular endothelial growth factor and its receptor flt-1 in the ovine pituitary gland: Expression is independent of hypothalamic control (1997) Mol Cell Endocrinol, 134, pp. 91-100
  • Arzt, E., Buric, R., Stelzer, G., Stalla, J., Sauer, J., Renner, U., Stalla, G.K., Interleukin involvement in anterior pituitary cell growth regulation: Effects of IL-2 and IL-6 (1993) Endocrinology, 132, pp. 459-467
  • Hori, S., Hayashi, N., Fukuoda, J., Kurimoto, M., Hamada, H., Miyajima, K., Nagai, S., Endo, S., Folliculostellate cell tumor in the pituitary gland (2009) Neuropathology, 29, pp. 78-80
  • Roncaroli, F., Scheithauer, B.W., Cenacchi, G., Horvath, E., Kovacs, K., Lloyd, R.V., Abell-Aleff, P., Yates, A.J., 'Spindle cell oncocytoma' of the adenohypophysis. A tumor of folliculostellate cells? (2002) Am J Surg Pathol, 26, pp. 1048-1055
  • Iwaki, T., Kondo, A., Takeshita, I., Nakagaki, H., Kitamura, K., Tateishi, J., Proliferating potential of folliculo-stellate cells in human pituitary adenomas. Immunohistochemical and electron microscopic analysis (1986) Acta Neuropathol, 71, pp. 233-242
  • Höfler, H., Walter, G.F., Denk, H., Immunohistochemistry of folliculo-stellate cells in normal human adenohypopheses and in pituitary adenomas (1984) Acta Neuropathol, 65, pp. 35-40
  • Farnoud, M.R., Kujas, M., Derome, P., Racadot, J., Peillon, F., Li, J.Y., Interactions between normal and tumoral tissues at the boundary of human pituitary adenomas (1994) Virchows Arch, 424, pp. 75-82
  • Yamashita, M., Qian, Z.R., Sano, T., Horvath, E., Kovacs, K., Immunohistochemical study on so-called follicular cells and folliculostellate cells in the human adenohypophysis (2005) Pathol Int, 55, pp. 244-247

Citas:

---------- APA ----------
Schaaf, C., Shan, B., Onofri, C., Stalla, G.K., Arzt, E., Schilling, T., Perone, M.J.,..., Renner, U. (2010) . Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells. Neuroendocrinology, 91(2), 200-210.
http://dx.doi.org/10.1159/000287236
---------- CHICAGO ----------
Schaaf, C., Shan, B., Onofri, C., Stalla, G.K., Arzt, E., Schilling, T., et al. "Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells" . Neuroendocrinology 91, no. 2 (2010) : 200-210.
http://dx.doi.org/10.1159/000287236
---------- MLA ----------
Schaaf, C., Shan, B., Onofri, C., Stalla, G.K., Arzt, E., Schilling, T., et al. "Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells" . Neuroendocrinology, vol. 91, no. 2, 2010, pp. 200-210.
http://dx.doi.org/10.1159/000287236
---------- VANCOUVER ----------
Schaaf, C., Shan, B., Onofri, C., Stalla, G.K., Arzt, E., Schilling, T., et al. Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells. Neuroendocrinology. 2010;91(2):200-210.
http://dx.doi.org/10.1159/000287236