Artículo
Abstract:
We have studied the role of mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) on salt appetite developed by deoxycorticosterone acetate (DOCA) treated rats. To this end, we measured the effects of DOCA given on alternate days on (1) salt intake; (2) MR and GR in hippocampus (HIPPO), amygdala (AMYG), and hypothalamus (HT); (3) the activity of ornithine decarboxylase (ODC), a OR-mediated response, and (4) the salt intake after treatment with the antiglucocorticoid RU 486 or the antimineralocorticoid ZK 91587. First, we demonstrated that 10 but not 1 mg DOCA induced natriogenesis. Forty-eight hours after adrenalectomy and 24 h after the last DOCA injection, 10 but not 1 mg hormone reduced binding to OR in HIPPO, AMYG, and HT. Both doses of DOCA also reduced the binding to MR in HIPPO, without changes in AMYG; in HT the 1-mg dose was without effect, but the natriogenic dose (10 mg) highly increased binding of [3H]-corticosterone to MR. Scatchard analysis demonstrated increased B(max) and K(d) values in the HT of DOCA-treated rats. Occupation of GR by DOCA did not stimulate the ODC activity, in contrast to the few-fold increment effected by the glucocorticoid dexamethasone. Also, administration of RU 486 did not inhibit the salt intake promoted by DOCA, in contrast to ZK 91587 which partly delayed the natriogenic effect of DOCA. It is suggested that brain MR are involved in the natriogenic effect of DOCA, whereas the role of GR is inconclusive. Although GR were occupied by DOCA, this compound did not show agonistic properties regarding induction of ODC nor antagonism of dexamethasone-induced ODC, indicating that DOCA (or its metabolites) rendered inactive GR.
Registro:
| Documento: | Artículo |
| Título: | Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| Autor: | Vallee, S.M.; Grillo, C.A.; Gonzalez, S.; Cosen-Binker, L.; De Kloet, E.R.; McEwen, B.S.; De Nicola, A.F. |
| Filiación: | Lab. of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina, Experimental CONICET, Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | Adrenal steroid receptors; Adrenal steroids; Ornithine decarboxylase activity; Salt intake; 15beta,16beta methylenemexrenone; corticosterone; deoxycorticosterone acetate; dexamethasone; glucocorticoid antagonist; glucocorticoid receptor; mifepristone; mineralocorticoid antagonist; mineralocorticoid receptor; ornithine decarboxylase; adrenalectomy; amygdaloid nucleus; animal experiment; animal tissue; article; controlled study; enzyme activity; hippocampus; hypothalamus; male; nonhuman; priority journal; rat; receptor binding; salt intake; scatchard plot; Amygdala; Animal; Appetite; Brain; Desoxycorticosterone; Hippocampus; Hypothalamus; Male; Mifepristone; Ornithine Decarboxylase; Rats; Rats, Sprague-Dawley; Receptors, Glucocorticoid; Receptors, Mineralocorticoid; Sodium Chloride, Dietary; Spironolactone |
| Año: | 1995 |
| Volumen: | 61 |
| Número: | 2 |
| Página de inicio: | 117 |
| Página de fin: | 124 |
| Título revista: | Neuroendocrinology |
| Título revista abreviado: | NEUROENDOCRINOLOGY |
| ISSN: | 00283835 |
| CODEN: | NUNDA |
| CAS: | 15beta,16beta methylenemexrenone, 84542-26-7; corticosterone, 50-22-6; deoxycorticosterone acetate, 56-47-3; dexamethasone, 50-02-2; mifepristone, 84371-65-3; ornithine decarboxylase, 9024-60-6; Desoxycorticosterone, 64-85-7; Mifepristone, 84371-65-3; Ornithine Decarboxylase, EC 4.1.1.17; Receptors, Glucocorticoid; Receptors, Mineralocorticoid; Sodium Chloride, Dietary; Spironolactone, 52-01-7; ZK 91587, 84542-26-7 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee |
Citas:
---------- APA ----------
Vallee, S.M., Grillo, C.A., Gonzalez, S., Cosen-Binker, L., De Kloet, E.R., McEwen, B.S. & De Nicola, A.F. (1995) . Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake. Neuroendocrinology, 61(2), 117-124.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee [ ]
---------- CHICAGO ----------
Vallee, S.M., Grillo, C.A., Gonzalez, S., Cosen-Binker, L., De Kloet, E.R., McEwen, B.S., et al. "Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake" . Neuroendocrinology 61, no. 2 (1995) : 117-124.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee [ ]
---------- MLA ----------
Vallee, S.M., Grillo, C.A., Gonzalez, S., Cosen-Binker, L., De Kloet, E.R., McEwen, B.S., et al. "Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake" . Neuroendocrinology, vol. 61, no. 2, 1995, pp. 117-124.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee [ ]
---------- VANCOUVER ----------
Vallee, S.M., Grillo, C.A., Gonzalez, S., Cosen-Binker, L., De Kloet, E.R., McEwen, B.S., et al. Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake. NEUROENDOCRINOLOGY. 1995;61(2):117-124.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee [ ]
