We evaluated the 'in vitro' effect of a selective peripheral benodiazepine (BZD) receptor agonist, Ro 5-4864, on basal and hCG-stimulated androgen production by testicular interstitial cell suspensions. Ro 5-4864 (10-9-10-5 M) induced a significant increment of basal testosterone release into the medium. In addition, under conditions of hCG stimulation, Ro 5-4864 (10-7 M) induced a potentiated response to the gonadotropin in a dose-dependent manner. The selective peripheral BZD antagonist PK 11195 fully prevented the stimulatory effect of Ro 5-4864. On the other hand, clonazepam, a central BZD agonist, failed to affect androgen production significantly, whereas diazepam (10-5-10-4 M), which binds to both central and peripheral BZD receptors, was able to induce a significant increment of basal and hCG-stimulated testosterone production. These results suggest that under our experimental conditions Ro 5-4864 exerts an effect on testicular steroidogenesis, presumably through binding to the previously described peripheral-type BZD receptor.
Documento: | Artículo |
Título: | Testicular interstitial cells as targets for peripheral benzodiazepines |
Autor: | Ritta, M.N.; Calandra, R.S. |
Filiación: | Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina |
Palabras clave: | 4' chlorodiazepam; androgen; benzodiazepine; chorionic gonadotropin; clonazepam; diazepam; n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide; testosterone; animal cell; Leydig cell; male; nonhuman; priority journal; rat; steroidogenesis; testis; topical drug administration |
Año: | 1989 |
Volumen: | 49 |
Número: | 3 |
Página de inicio: | 262 |
Página de fin: | 266 |
DOI: | http://dx.doi.org/10.1159/000125126 |
Título revista: | Neuroendocrinology |
Título revista abreviado: | NEUROENDOCRINOLOGY |
ISSN: | 00283835 |
CODEN: | NUNDA |
CAS: | 4' chlorodiazepam, 14439-61-3; benzodiazepine, 12794-10-4; chorionic gonadotropin, 9002-61-3; clonazepam, 1622-61-3; diazepam, 439-14-5; n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide, 85532-75-8; testosterone, 58-22-0 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v49_n3_p262_Ritta |