Artículo

Vacas, M.I.; Keller-Sarmiento, M.I.; Etchegoyen, G.S.; Pereyra, E.N.; Gimeno, M.F.; Cardinali, D.P. "Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis" (1987) Neuroendocrinology. 46(5):412-416
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Abstract:

The effect of lipoxygenase inhibition, leukotriene agonists and antagonists, and 5-hydroxy-6, 8, l 1, 14-eicosate-traenoic acid (5-HF.TE) was examined in the rat pineal gland in organ culture. To study melatonin secretion pineal explants were incubated for 6 h in tissue culture medium 199 with the different drugs. Melatonin concentration in the pineal gland and the medium was measured by RIA. Exposure of explants to norepinephrine (NE) brought about a 2-to 5-fold increase in both parameters, an effect that was reduced but not abolished, by the lipoxygenase inhibitor nordihydro-guaiaretic acid (NDGA: I0-5M). Lilly 171883 (10-5M) or FPL 55712 (10 5 M: Both antagonists of leukotrienes) reduced NE-induced melatonin production. Neither NDGA nor Lilly 171883 affected melatonin production in the absence of NE. Leukotrienes C4 and D4 increased melatonin release to the media at all concentrations tested (1-1, 000 n M) with a maximum effect at I nM(leukotriene C4) and 10 nM(leukotriene D4). Significantly higher tissue melatonin concentrations as compared to controls were observed after exposure of pineal explants to I and 100 nM of leukotriene C4, or 100 nM of leukotriene D4. Another 5-lipoxygenase metabolite, 5-HETE, increased pineal melatonin content at concentrations of 1, 10 and 100 nMwhereas only 1, 000 nM stimulated melatonin release. These results suggest that the 5-lipoxygenase pathway plays a significant role in NE-stimulated melatonin production by the rat pineal gland. © 1987 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis
Autor:Vacas, M.I.; Keller-Sarmiento, M.I.; Etchegoyen, G.S.; Pereyra, E.N.; Gimeno, M.F.; Cardinali, D.P.
Filiación:Centro de Estudios Farmacólogicos y de Principios Naturales (CEFAPRIN), Buenos Aires, Argentina
Palabras clave:Leukotriene antagonists; Leukotrienes; Lipoxygenase inhibition; Melatonin; Pineal organ cultures; 5 hydroxyicosatetraenoic acid; leukotriene; leukotriene c4; lipoxygenase; melatonin; noradrenalin; nordihydroguaiaretic acid; animal cell; cell culture; central nervous system; drug antagonism; drug comparison; endocrine system; in vitro study; nonhuman; pineal body; rat; Acetophenones; Animals; Arachidonate 5-Lipoxygenase; Arachidonate Lipoxygenases; Chromones; Hydroxyeicosatetraenoic Acids; Male; Melatonin; Nordihydroguaiaretic Acid; Norepinephrine; Organ Culture Techniques; Pineal Gland; Rats; Rats, Inbred Strains; SRS-A; Tetrazoles
Año:1987
Volumen:46
Número:5
Página de inicio:412
Página de fin:416
DOI: http://dx.doi.org/10.1159/000124854
Título revista:Neuroendocrinology
Título revista abreviado:Neuroendocrinology
ISSN:00283835
CAS:5 hydroxyicosatetraenoic acid, 72255-35-7; leukotriene C4, 72025-60-6; lipoxygenase, 9027-17-2, 9029-60-1; melatonin, 73-31-4; noradrenalin, 1407-84-7, 51-41-2; nordihydroguaiaretic acid, 500-38-9; 5-hydroxy-6,8,11,14-eicosatetraenoic acid, 72255-35-7; Acetophenones; Arachidonate 5-Lipoxygenase, 1.13.11.34; Arachidonate Lipoxygenases, 1.13.11.-; Chromones; FPL 55712, 40786-08-1; Hydroxyeicosatetraenoic Acids; LY 171883, 88107-10-2; Melatonin, 73-31-4; Nordihydroguaiaretic Acid, 500-38-9; Norepinephrine, 51-41-2; SRS-A; Tetrazoles
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v46_n5_p412_Vacas

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Citas:

---------- APA ----------
Vacas, M.I., Keller-Sarmiento, M.I., Etchegoyen, G.S., Pereyra, E.N., Gimeno, M.F. & Cardinali, D.P. (1987) . Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis. Neuroendocrinology, 46(5), 412-416.
http://dx.doi.org/10.1159/000124854
---------- CHICAGO ----------
Vacas, M.I., Keller-Sarmiento, M.I., Etchegoyen, G.S., Pereyra, E.N., Gimeno, M.F., Cardinali, D.P. "Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis" . Neuroendocrinology 46, no. 5 (1987) : 412-416.
http://dx.doi.org/10.1159/000124854
---------- MLA ----------
Vacas, M.I., Keller-Sarmiento, M.I., Etchegoyen, G.S., Pereyra, E.N., Gimeno, M.F., Cardinali, D.P. "Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis" . Neuroendocrinology, vol. 46, no. 5, 1987, pp. 412-416.
http://dx.doi.org/10.1159/000124854
---------- VANCOUVER ----------
Vacas, M.I., Keller-Sarmiento, M.I., Etchegoyen, G.S., Pereyra, E.N., Gimeno, M.F., Cardinali, D.P. Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis. Neuroendocrinology. 1987;46(5):412-416.
http://dx.doi.org/10.1159/000124854