Abstract:
The possible existence of pineal progesterone (P) receptors was assessed in 105,000 g supernatants of bovine pineal glands incubated with3H-P in the presence of 10 μM Cortisol. By charcoal adsorption analysis specific binding (defined as the difference between3H-P binding in the absence and presence of 1,000-fold excess of unlabeled P) attained its maximum after 60 min at 37 °C and after 5 h at 0°C. Binding was abolished by heating the cytosol or by pre-incubating it with trypsin. A single population of binding sites with a Kd = 1.64 nM and binding site concentration = 34 fmol/mg of cytosol protein was detectable by Scatchard analysis. In competition experiments the B50 progesterone values (nAf) were: P (2), 20β-dihydroproges-terone (12), 20α-dihydroprogesterone (25), estradiol (158), testosterone (675) and corticosterone (> 1,000). After sucrose gradient centrifugation of pineal cytosol incubated with3H-P a discrete peak of radioactivity in the 7-8 S region of the gradient was observed, and disappeared following incubation with excess unlabeled P. These results indicate that P receptors are present in the bovine pineal gland. © 1979 S. Karger AG, Basel.
Registro:
Documento: |
Artículo
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Título: | Characterization of a cytosol progesterone receptor in bovine pineal gland |
Autor: | Vacas, M.I.; Lowenstein, P.R.; Cardinali, D.P. |
Filiación: | Centro de Estudios Farmacológicos, de Principios Naturales (CEFAPRIN), Buenos Aires, Argentina
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Palabras clave: | Pineal gland Progesterone; Steroid receptors; 20alpha dihydroprogesterone; 20beta dihydroprogesterone; corticosterone; estradiol; progesterone; progesterone receptor; radioisotope; testosterone; animal experiment; cattle; central nervous system; cytosol; drug binding; drug comparison; in vitro study; pineal body; progesterone h 3; Animal; Binding, Competitive; Cattle; Cytosol; Kinetics; Pineal Gland; Progesterone; Receptors, Progesterone |
Año: | 1979
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Volumen: | 29
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Número: | 2
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Página de inicio: | 84
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Página de fin: | 89
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DOI: |
http://dx.doi.org/10.1159/000122909 |
Título revista: | Neuroendocrinology
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Título revista abreviado: | Neuroendocrinology
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ISSN: | 00283835
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CAS: | 20alpha dihydroprogesterone, 145-14-2; 20beta dihydroprogesterone, 145-15-3; corticosterone, 50-22-6; estradiol, 50-28-2; progesterone, 57-83-0; testosterone, 58-22-0; Progesterone, 57-83-0; Receptors, Progesterone
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v29_n2_p84_Vacas |
Referencias:
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Citas:
---------- APA ----------
Vacas, M.I., Lowenstein, P.R. & Cardinali, D.P.
(1979)
. Characterization of a cytosol progesterone receptor in bovine pineal gland. Neuroendocrinology, 29(2), 84-89.
http://dx.doi.org/10.1159/000122909---------- CHICAGO ----------
Vacas, M.I., Lowenstein, P.R., Cardinali, D.P.
"Characterization of a cytosol progesterone receptor in bovine pineal gland"
. Neuroendocrinology 29, no. 2
(1979) : 84-89.
http://dx.doi.org/10.1159/000122909---------- MLA ----------
Vacas, M.I., Lowenstein, P.R., Cardinali, D.P.
"Characterization of a cytosol progesterone receptor in bovine pineal gland"
. Neuroendocrinology, vol. 29, no. 2, 1979, pp. 84-89.
http://dx.doi.org/10.1159/000122909---------- VANCOUVER ----------
Vacas, M.I., Lowenstein, P.R., Cardinali, D.P. Characterization of a cytosol progesterone receptor in bovine pineal gland. Neuroendocrinology. 1979;29(2):84-89.
http://dx.doi.org/10.1159/000122909