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Vázquez, M.; Ben-Dov, C.; Lorenzi, H.; Moore, T.; Schijman, A.; Levin, M.J. "The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons" (2000) Proceedings of the National Academy of Sciences of the United States of America. 97(5):2128-2133
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Abstract:

The short interspersed repetitive element (SIRE) of Trypanosoma cruzi was first detected when comparing the sequences of loci that encode the TcP2β genes. It is present in about 1,500-3,000 copies per genome, depending on the strain, and it is distributed in all chromosomes. An initial analysis of SIRE sequences from 21 genomic fragments allowed us to derive a consensus nucleotide sequence and structure for the element, consisting of three regions (I, II, and III) each harboring distinctive features. Analysis of 158 transcribed SIREs demonstrates that the consensus is highly conserved. The sequences of 51 cDNAs show that SIRE is included in the 3' end of several mRNAs, always transcribed from the sense strand, contributing the polyadenylation site in 63% of the cases. This study led to the characterization of VIPER (vestigial interposed retroelement), a 2,326-bp- long unusual retroelement. VIPER's 5' end is formed by the first 182 bp of SIRE, whereas its 3' end is formed by the last 220 bp of the element. Both SIRE moieties are connected by a 1,924-bp-long fragment that carries a unique ORF encoding a complete reverse transcriptase-RNase H gene whose 15 C- terminal amino acids derive from codons specified by SIRE's region II. The amino acid sequence of VIPER's reverse transcriptase-RNase H shares significant homology to that of long terminal repeat retrotransposons. The fact that SIRE and VIPER sequences are found only in the T. cruzi genome may be of relevance for studies concerning the evolution and the genome flexibility of this protozoan parasite.

Registro:

Documento: Artículo
Título:The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons
Autor:Vázquez, M.; Ben-Dov, C.; Lorenzi, H.; Moore, T.; Schijman, A.; Levin, M.J.
Filiación:Lab. Biol. Molec. Enfermedad Chagas, Inst. Invest. Ing. Genet. Y Biol. M., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Research Genetics, 2130 Memorial Parkway, Huntsville, AL 35801, United States
Foundation Jean Dausset, Ctr. d'Etude du Polymorphisme Humain, 27, rue Juliette Dodu, 75010 Paris, France
Inst. Invest. Ing. Genet. Y Biol. M., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:complementary DNA; messenger RNA; ribonuclease H; RNA directed DNA polymerase; amino acid sequence; article; controlled study; DNA sequence; genetic transcription; long terminal repeat; nonhuman; nucleotide sequence; polyadenylation; priority journal; retroposon; reverse transcription polymerase chain reaction; sequence homology; short interspersed repeat; species difference; Trypanosoma cruzi; Amino Acid Sequence; Animals; Base Sequence; Consensus Sequence; DNA, Protozoan; Gene Expression; Molecular Sequence Data; Protozoan Proteins; Retroelements; Ribosomal Proteins; Sequence Homology, Amino Acid; Short Interspersed Nucleotide Elements; Species Specificity; Terminal Repeat Sequences; Trypanosoma cruzi; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:2000
Volumen:97
Número:5
Página de inicio:2128
Página de fin:2133
DOI: http://dx.doi.org/10.1073/pnas.050578397
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:DNA, Protozoan; Protozoan Proteins; Retroelements; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v97_n5_p2128_Vazquez

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Citas:

---------- APA ----------
Vázquez, M., Ben-Dov, C., Lorenzi, H., Moore, T., Schijman, A. & Levin, M.J. (2000) . The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons. Proceedings of the National Academy of Sciences of the United States of America, 97(5), 2128-2133.
http://dx.doi.org/10.1073/pnas.050578397
---------- CHICAGO ----------
Vázquez, M., Ben-Dov, C., Lorenzi, H., Moore, T., Schijman, A., Levin, M.J. "The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons" . Proceedings of the National Academy of Sciences of the United States of America 97, no. 5 (2000) : 2128-2133.
http://dx.doi.org/10.1073/pnas.050578397
---------- MLA ----------
Vázquez, M., Ben-Dov, C., Lorenzi, H., Moore, T., Schijman, A., Levin, M.J. "The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons" . Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 5, 2000, pp. 2128-2133.
http://dx.doi.org/10.1073/pnas.050578397
---------- VANCOUVER ----------
Vázquez, M., Ben-Dov, C., Lorenzi, H., Moore, T., Schijman, A., Levin, M.J. The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons. Proc. Natl. Acad. Sci. U. S. A. 2000;97(5):2128-2133.
http://dx.doi.org/10.1073/pnas.050578397